[1]丁 威,邢 军,魏全伟,等.二花脸猪卵巢卵泡形成和早期发育过程中雌二醇和孕酮含量变化及其受体定位[J].江苏农业学报,2016,(02):383-389.[doi:10.3969/j.issn.1000-4440.2016.02.023]
 DING Wei,XING Jun,WEI Quan-wei,et al.Changes in concentrations of estrodiol and progesterone during ovarian follicular development and localization of ovarian estradiol receptor(ER)and progesterone receptor(PR)in the fetal and neonatal Erhualian swines[J].,2016,(02):383-389.[doi:10.3969/j.issn.1000-4440.2016.02.023]
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二花脸猪卵巢卵泡形成和早期发育过程中雌二醇和孕酮含量变化及其受体定位()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年02期
页码:
383-389
栏目:
畜牧兽医·水产养殖
出版日期:
2016-03-20

文章信息/Info

Title:
Changes in concentrations of estrodiol and progesterone during ovarian follicular development and localization of ovarian estradiol receptor(ER)and progesterone receptor(PR)in the fetal and neonatal Erhualian swines
作者:
丁 威12 邢 军12 魏全伟2 石放雄2
1.江苏农林职业技术学院, 江苏 句容 212400; 2.南京农业大学动物科技学院, 江苏 南京 210095
Author(s):
DING Wei12 XING Jun12 WEI Quan-wei2 SHI Fang-xiong2
1.Jiangsu Polytechnic College of Agriculture and Forestry,Jurong 212400,China; 2.College of Animal Science,Nanjing Agricultural University,Nanjing 210095,China
关键词:
卵巢 雌二醇 孕酮
Keywords:
pig ovary estradiol progesterone
分类号:
S828
DOI:
10.3969/j.issn.1000-4440.2016.02.023
文献标志码:
A
摘要:
为了研究雌二醇(E2)和孕酮(P4)在猪卵巢卵泡形成和早期发育过程中可能的作用,本试验以胎70日龄、90日龄和新生1日龄3个阶段的母猪为试验动物,通过免疫组织化学方法对胎猪和新生仔猪卵巢组织中的雌二醇受体(ER)和孕激素受体(PR)进行定位分析。通过放射免疫测定法对胎猪和新生仔猪的卵巢组织和血清中E2及P4含量进行测定,并分析E2和P4水平与猪的卵泡形成的相关性。结果显示:在猪早期阶段卵巢中ER主要在卵巢表皮细胞,卵母细胞巢中的卵原细胞和卵母细胞,以及原始卵泡、初级卵泡和次级卵泡的卵母细胞和颗粒细胞的核中表达,PR主要在卵巢表皮细胞,卵母细胞胞核,原始卵泡和初级卵泡的颗粒细胞的核中表达; 随着胎龄的增加,卵巢中的E2和P4水平下降,并且与卵泡的形成和发育呈负相关。说明出生前猪卵巢组织中的E2和P4含量变化可能影响猪卵巢卵泡的形成和早期发育。
Abstract:
To investigate the effects of estrodiol and progesterone on porcine ovarian follicular formation and development, the ovaries of 70 days postcoitum(dpc), 90 dpc, and 1 day postpartum(dpp)were used to detect the localization of estradiol receptor(ER)and progesteron receptor(PR)by immunohistochemistry, respectively. ERs were expressed primarily in ovarian epidermal cells, oogonia and oocytes in the egg nests, nuclei of oocytes and granulosa cells of follicles from primordial to secondary stages. The expression pattern of PR was similar to the ERs. The radioimmunoassay(RIA)findings implied that ovarian levels of estrodiol and progesterone were reduced as the fetus aged. There were negative correlations between the number of follicles and the concentrations of estrodiol and progesterone. Taken together, our findings suggested that estrodiol and progesterone might play a potential role in follicular formation and development in fetal and neonatal pigs.

参考文献/References:

[1] PEPLING M E, SPRADLING A C. Female mouse germ cells form synchronously dividing cysts [J]. Development,1998,125:3323-3328.
[2] PEPLING M E, D E CUEVAS M, SPRADLING A C. Germline cysts: a conserved phase of germ cell development? [J]. Trends Cell Biol, 1999, 9:257-262.
[3] JOHNSON J, BAGLEY J, SKAZNIK-WIKIEL M, et al.Oocyte generation in adult mammalian ovaries by putative germ cells in bone marrow and peripheral blood [J]. Cell, 2005, 122: 303-315.
[4] JOHNSON J, CANNING J, KANEKO T, et al.Germline stem cells and follicular renewal in the postnatal mammalian ovary [J]. Nature, 2004, 428: 145-150.
[5] CHEN Y, JEFFERSON W N, NEWBOLD R R, et al. Estradiol, progesterone, and genistein inhibit oocyte nest breakdown and primordial follicle assembly in the neonatal mouse ovary in vitro and in vivo [J]. Endocrinology, 2007, 148: 3580-3590.
[6] NILSSON E E, SKINNER M K. Progesterone regulation of primordial follicle assembly in bovine fetal ovaries [J]. Mol Cell Endocrinol, 2009, 313(1-2): 9-16.
[7] JEFFERSON W, NEWBOLD R, PADILLA-BANKS E, et al. Neonatal genistein treatment alters ovarian differentiation in the mouse inhibition of oocyte nest breakdown and increased oocyte survival [J]. Biol Reprod, 2006, 74: 161-168.
[8] KNAPCZYK K, DUDA M, SZAFRANSKA B, et al. Immunolocalisation of oestrogen receptors alpha(ERalpha)and beta(ERbeta)in porcine embryos and fetuses at different stages of gestation [J]. Acta Vet Hung, 2008, 56:221-233.
[9] KNAPCZYK K, DUDA M, DURLEJ M, et al.Expression of estrogen receptor alpha(ERalpha)and estrogen receptor beta(ERbeta)in the ovarian follicles and corpora lutea of pregnant swine [J]. Domest Anim Endocrinol, 2008, 35:170-179.
[10] D'HAESELEER M, SIMOENS P, VAN DEN BROECK W. Cell-specific localization of progesterone receptors in the bovine ovary at different stages of the oestrous cycle [J].Anim Reprod Sci, 2007, 98(3-4):271-81.
[11] SOMCZYN'SKA M,MAGORZATA K. Localization of the progesterone receptor in the porcine ovary [J]. Acta Histochemica, 2007, 102(2):183-191.
[12] JENNIFER L J, DEREK A H, LAUREL D Q, et al. Oestrogen receptor α and β, and rogen receptor and progesterone receptor mRNA and protein localisation within the developing ovary and in small growing follicles of sheep [J]. Reproduction, 2006, 131: 81-92.
[13] LUBAHN D B, MOYER J S, GOLDING T S, et al.Alteration of reproductive function but not prenatal sexual development after insertional disruption of the mouse estrogen receptor gene [J]. Proc Natl Acad Sci USA, 1993, 90: 11162-11166.
[14] DUPONT S, KRUST A, GANSMULLER A, et al. Effect of single and compound knockouts of estrogen receptors α(ERα)and β(ERβ)on mouse reproductive phenotypes [J]. Development, 2000, 127: 4277-4291.
[15] LUN S, SMITH P, LUNDY T, et al. Steroid contents and steroidogenesis in vitro by the developing gonad and mesonephros around sexual differentiation in fetal sheep [J]. J Reprod Fertil, 1998, 114(1): 131-139.
[16] JEFFERSON W N, COUSE J F, BANKS E P, et al. Expression of estrogen receptor β is developmentally regulated in reproductive tissues of male and female mice [J]. Biol Reprod, 2000, 62(2): 310-317.
[17] SLOMCZYNSKA M, WOZNIAK J.Differential distribution of estrogen receptor β and estrogen receptor α in the porcine ovary [J]. Exp Clin Endocrinol Diabetes, 2001, 109(4): 238-244.
[18] PELLETIER G, LABRIE C, LABRIE F. Localization of oestrogen receptor α, oestrogen receptor β and androgen receptors in the rat reproductive organs [J]. J Endocrinol, 2000, 165(2): 359-370.
[19] PELLTIER G, EL-ALFY M. Immunocytochemical localization of estrogen receptors α and β in the human reproductive organ [J]. J Clin Endocrinol Metabol, 2000, 85(12): 4835-4840.
[20] PEPE G J, BILLIAR R B, LEAVITT M G, et al. Expression of estrogen receptors α and β in the baboon fetal ovary [J]. Biol Reprod, 2002, 66(4):1054-1060.
[21] JENNIFER L JUENGEL, DEREK A HEATH, LAUREL D QUIRKE, et al. Oestrogen receptor α and β, androgen receptor and progesterone receptor mRNA and protein localization within the developing ovary and in small growing follicles of sheep [J]. Reproduction, 2006, 131: 81-92.
[22] GRECO T, PAYNE A H. Ontogeny of expression of the genes for steroidogenic enzymes P450 side-chain cleavage, 3 beta-hydroxysteroid dehydrogenase, P450 17 alpha-hydroxylase/C17-20 lyase, and P450 aromatase in fetal mouse gonads[J]. Endocrinology, 1994, 135(1): 262-268.
[23] COUSE J F,KORACH K S. Estrogen receptor null mice: what have we learned and where will they lead us? [J]. Endocr Rev, 1999, 20(3): 358-417.
[24] HUNTER R H F. Sex determination, differentiation and intersexuality in placental mammals[M]. Cambridge: Cambridge University Press, 1995.
[25] YANG M Y, FORTUNE J E. The capacity of primordial follicles in fetal bovine ovaries to initiate growth in vitro develops during mid-gestation and is associated with meiotic arrest of oocytes [J]. Biol Reprod, 2008, 78: 1153-1161.
[26] ECHTERNKAMP S E, VONNAHME K A, GREEN J A, et al. Increased vascular endothelial growth factor and pregnancy-associated glycoproteins, but not insulin-like growth factor-I, in maternal blood of cows gestating twin fetuses [J]. J Anim Sci, 2006, 84: 2057-2064.
[27] LYDON J P, DEMAYO F J, FUNK C R, et al. Mice lacking progesterone receptor exhibit pleiotropic reproductive abnormalities [J]. Genes Dev, 1995, 9: 2266-2278.
[28] JERICEVIC B M, MULLINAX R A, DEMAYO F J, et al.Subgroup of reproductive functions of progesterone mediated by progesterone receptor-B isoform [J]. Science, 2000, 289: 1751-1754.
[29] JERICEVIC B M, LYDON J P, DEMAYO F J, et al. Defective mammary gland morphogenesis in mice lacking the progesterone receptor B isoform[J]. Proc Natl Acad Sci USA, 2003, 100: 9744-9749.
[30] LEI L, JIN S, MAYO K E, et al. The interactions between the stimulatory effect of follicle-stimulating hormone and the inhibitory effect of estrogen on mouse primordial folliculogenesis [J]. Biol Reprod, 2010, 82(1):13-22.
[31] KEZELE P, SKINNER M K. Regulation of ovarian primordial follicle assembly and development by estrogen and progesterone: endocrine model of follicle assembly [J]. Endocrinology, 2003, 144(8): 3329-3337.

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备注/Memo

备注/Memo:
收稿日期:2015-08-31 基金项目:江苏省自然科学基金项目(BK2011499) 作者简介:丁 威(1979-),男,黑龙江省巴彦县人,博士研究生,副教授,主要从事猪的卵巢卵泡发育调控机制研究。(Tel)0511-87292202;(E-mail)qishi6598@126.com 通讯作者:石放雄,(E-mail)fxshi@njau.edu.cn
更新日期/Last Update: 2016-03-20