[1]李晓霞,贾淼,金君华,等.原料乳中金黄色葡萄球菌环介导等温扩增（LAMP）检测方法的建立及应用[J].江苏农业学报,2017,(05):1171-1175.[doi:doi:10.3969/j.issn.1000-4440.2017.05.032]
　LI Xiao-xia,JIA Miao,JIN Jun-hua,et al.Establishment and application of loop-mediated isothermal amplification(LAMP) of Staphylococcus aureus in raw milk[J].,2017,(05):1171-1175.[doi:doi:10.3969/j.issn.1000-4440.2017.05.032]
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原料乳中金黄色葡萄球菌环介导等温扩增（LAMP）检测方法的建立及应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
1171-1175
栏目:
加工贮藏·质量安全
出版日期:
2017-10-30

文章信息/Info

Title:
Establishment and application of loop-mediated isothermal amplification(LAMP) of Staphylococcus aureus in raw milk
作者:
李晓霞; 贾淼; 金君华; 张红星; 刘慧; 谢远红
（北京农学院食品科学与工程学院，农产品有害微生物及农残安全检测与控制北京市重点实验室/食品质量与安全北京实验室，北京102206）
Author(s):
LI Xiao-xia; JIA Miao; JIN Jun-hua; ZHANG Hong-xing; LIU Hui; XIE Yuan-hong
(College of Food Science and Engineering, Beijing Agricultural College/Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products/Beijing Laboratory of Food Quality and Safety, Beijing 102206, China)
关键词:
环介导等温扩增（LAMP）; 金黄色葡萄球菌; 耐热核酸酶基因(nuc); 灵敏度; 特异性
Keywords:
loop-mediated isothermal amplification（LAMP）; Staphylococcus aureus; thermostable nuclease gene; sensitivity; specificity
分类号:
TS207.4
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.032
文献标志码:
A
摘要:
为了建立原料乳中金黄色葡萄球菌的环介导等温扩增（LAMP）检测方法，本研究根据金黄色葡萄球菌的耐热核酸酶基因(nuc)在保守区域设计特异性引物，建立LAMP检测方法，并用于原料乳中金黄色葡萄球菌的快速检测。结果表明，针对金黄色葡萄球菌的基因组DNA，使用设计合成的4条引物和建立的LAMP方法能够有效检测金黄色葡萄球菌DNA，每250 μl反应体系的灵敏度为1100 fg，并且此方法针对金黄色葡萄球菌具有良好的特异性。针对原料乳中金黄色葡萄球菌的污染，建立的LAMP检测方法的检测限为67 CFU/ml。本研究结果为进一步将LAMP方法应用于食品中金黄色葡萄球菌的安全检测奠定了基础。
Abstract:
The loop-mediated isothermal amplification(LAMP) method was established and applied in the rapid detection of Staphylococcus aureus in raw milk according to the conserved sequence of the heat-tolerant nuclease gene(nuc). The genomic DNA of S. aureus could be detected using the four primers designed and synthesized, and the detection limit was 1100 fg per 250 μl reaction system. The method was S. aureus specific, with the detection limit of 67 CFU/ml in raw milk. This study provided a basis for further application of LAMP method in the safety detection of S.aureus in food.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2017-04-25 基金项目：北京市长城学者培养计划项目（CIT&TCD20140315）；北京农学院研究生改革与发展项目（2016YJS048） 作者简介：李晓霞（1990-），女，甘肃定西人，硕士研究生，主要从事食品生物检测研究。（Tel）15652319099；（E-mail）lixiaoxia0154 @163.com 通讯作者：谢远红，(Tel)010-80761345；(E-mail) xieyuanh@163.com
更新日期/Last Update: 2017-11-03