[1]张娇娇,吴海虹,孙晋跃,等.超声波联合乳酸对金黄色葡萄球菌的杀菌效果[J].江苏农业学报,2022,38(06):1686-1693.[doi:doi:10.3969/j.issn.1000-4440.2022.06.027]
　ZHANG Jiao-jiao,WU Hai-hong,SUN Jin-yue,et al.Bactericidal effect of ultrasound combined with lactic acid against Staphylococcus aureus[J].,2022,38(06):1686-1693.[doi:doi:10.3969/j.issn.1000-4440.2022.06.027]
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超声波联合乳酸对金黄色葡萄球菌的杀菌效果()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年06期
页码:
1686-1693
栏目:
加工贮藏·质量安全
出版日期:
2022-12-31

文章信息/Info

Title:
Bactericidal effect of ultrasound combined with lactic acid against Staphylococcus aureus
作者:
张娇娇1; 吴海虹2; 孙晋跃2; 孙远成3; 孙芝兰2; 刘芳1; 2; 徐为民2
（1.江苏大学食品与生物工程学院，江苏镇江212013；2.江苏省农业科学院农产品加工研究所，江苏南京210014；3.徐州荣炷食品有限公司，江苏徐州221000）
Author(s):
ZHANG Jiao-jiao1; WU Hai-hong2; SUN Jin-yue2; SUN Yuan-cheng3; SUN Zhi-lan2; LIU Fang1; 2; XU Wei-min2
(1.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;2.Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.Xuzhou Rongzhuo Food Co., Ltd.， Xuzhou 221000, China)
关键词:
超声波; 乳酸; 金黄色葡萄球菌; 羊肉
Keywords:
ultrasound; lactic acid; Staphylococcus aureus; mutton
分类号:
TS251.5+3
DOI:
doi:10.3969/j.issn.1000-4440.2022.06.027
文献标志码:
A
摘要:
研究超声波（US，400 W，50 kHz）、乳酸（LA,质量分数为0.5%, 1.0%）和超声波联合乳酸处理（US+0.5% LA，US+1.0% LA）对游离和生物膜状态下的金黄色葡萄球菌的抗菌和抗生物膜机制，并且选用US+0.5% LA对羊肉上的金黄色葡萄球菌进行杀菌处理。结果显示，超声波联合乳酸处理对金黄色葡萄球菌的杀菌作用明显，显著降低了生物膜胞外多糖的含量，破坏了生物膜的胞外基质（P<0.05）；通过扫描电子显微镜观察发现，超声波联合乳酸处理使得菌体细胞产生了严重的凹陷；激光共聚焦电镜和核酸泄露试验结果表明，超声波联合乳酸处理对菌体的细胞膜造成了严重破坏，使其通透性显著增加，进而造成内部核酸大量泄露；US+0.5% LA处理可以显著地控制羊肉上金黄色葡萄球菌的生长。因此，超声波联合乳酸处理通过影响菌体细胞膜的完整性、改变细胞膜的通透性，使生物膜胞外多糖含量降低及内部核酸泄露，进而发挥杀菌作用。
Abstract:
The antibacterial and anti-biofilm mechanism of ultrasound treatment (US, 400 W, 50 kHz), lactic acid treatment (LA, the mass fractions were 0.5% and 1.0%) and ultrasound combined with lactic acid treatment (US+0.5% LA, US+1.0% LA) against planktonic and biofilm cell-type Staphylococcus aureus were studied. In addition, treatment of US+0.5% LA was used to sterilize the S. aureus on mutton. The results showed that, US+LA treatment had obvious synergistic bactericidal effect on S. aureus cells and decreased the content of exopolysaccharides (EPS) in the biofilm significantly (P<0.05), while destroyed the extracellular matrix of biofilm. It was discovered by scanning electron microscope that, US+LA treatment caused severe sunken of the bacterial cells. Results of laser confocal electron microscopy and nucleic acid leakage showed that, US+LA treatment caused severe damage to the cell membrane of the bacteria, which increased the permeability of their cell membranes significantly and caused massive leakage of internal nucleic acids. In addition, US+0.5% LA treatment could control the growth of S. aureus on mutton significantly. Therefore, US+LA treatment plays a synergistic bactericidal role through affecting the integrity of the bacterial cell membranes, changing the permeability of cell membranes, decreasing EPS content in the biofilm and causing the leakage of inner nucleic acid.

参考文献/References:

[1]马惠敏,王振宇,黄彩燕,等. 包装对羊肉冷藏过程中热休克蛋白表达及羊肉品质的影响[J]. 肉类研究, 2021, 35(3): 35-40.
[2]薛婉瑞，张海生，赵鑫帅,等． 复合天然保鲜剂对冷却羊肉的保鲜效果[J]. 食品与发酵工业，2020，46(5):240-247.
[3]刘婷,张瑞，吴建平,等． 牛至精油结合不同包装方式对冷鲜羊肉保鲜效果的影响[J]. 食品与发酵工业，2020，46（4）：139-145.
[4]李鹏. 2018年酒泉市部分地区市售牛羊肉食源性致病菌污染情况调查与分析[J]. 中国草食动物科学, 2020, 40(5): 59-61.
[5]COSTA R S, LINS M O, HYARIC M L, et al. In vitro antibacterial effects of Zanthoxylum tingoassuiba root bark extracts and two of its alkaloids against multiresistant Staphylococcus aureus [J]. Rev Bras Farmacogn, 2016, 27(2): 195-198.
[6]宁亚维,苏丹,付浴男,等. 抗菌肽brevilaterin与ε-聚赖氨酸对金黄色葡萄球菌的协同抑菌机理[J]. 食品科学, 2020, 41(5): 15-22.
[7]戴雨芸，李超，袁中伟,等. 香芹酚抑制金黄色葡萄球菌生物被膜的形成[J]. 微生物学通报, 2020, 47(3): 813-820.
[8]SHIVAEE A, RAJABI S, FARAHANI H E, et al. Effect of sub-lethal doses of nisin on Staphylococcus aureus toxin production and biofilm formation [J]. Toxicon, 2021, 197(4)：1-5.
[9]BAI M, LI C Z, CUI H Y, et al. Preparation of self-assembling Litsea cubeba essential oil/ diphenylalanine peptide micro/nanotubes with enhanced antibacterial properties against Staphylococcus aureus biofilm [J]. LWT, 2021, 146(3): 111394.
[10]GONZALEZ-AANDOS E, MARTINEZ-LAORDEN A, PEREZ-ARNEDO I. Efficacy of combinations of lactic acid and potassium sorbate against Listeria monocytogenes in chicken stored under modified atmospheres [J]. Food Microbiol, 2020, 93: 103596.
[11]ANANG D M, RUSUL G, LING F H, et al. Inhibitory effects of lactic acid and lauricidin on spoilage organisms of chicken breast during storage at chilled temperature [J]. Int J Food Microbiol, 2010, 144(1): 152-159.
[12]YOUSSEF M K, YANG X, BADONI M, et al. Effects of spray volume, type of surface tissue and inoculum level on the survival of Escherichia coli on beef sprayed with 5% lactic acid [J]. Food Control, 2012, 25(2): 717-722.
[13]LI Z Y, GUO R X, WANG F, et al. Inactivation of Salmonella enteritidis on eggshells by lactic acid spray [J]. Food Control, 2019, 104: 201-207.
[14]迟媛,弓敏,马艳秋,等. 超声波协同次氯酸钠杀灭腐败菌效果与动力学研究[J]. 农业机械学报, 2020, 51（7)：372-381.
[15]毕秀芳,陈丽伊,潘泓桦,等. 超声波波结合nisin对胡萝卜汁的杀菌效果[J]. 中国食品学报, 2018, 18(1): 211-216.
[16]盖作启. 热-超声波联合处理对牛奶的杀菌效果及对其品质影响的研究[D]. 广州:华南理工大学, 2009.
[17]CICHOSKI A J, FLORES D, MENEZES C, et al. Ultrasound and slightly acid electrolyzed water application: an efficient combination to reduce the bacterial counts of chicken breast during pre-chilling [J]. Int J Food Microbiol, 2019, 301: 27-33.
[18]YU H, LIU Y, YANG F W, et al. Synergistic efficacy of high-intensity ultrasound and chlorine dioxide combination for Staphylococcus aureus biofilm control [J]. Food Control, 2021, 122: 107822.
[19]索标,赵雅健,李真,等. 单辛酸甘油酯对即食湿面中金黄色葡萄球菌的抑制模型[J]. 中国食品学报, 2019, 19(5):191-200.
[20]蔡林林,胡海静,衣晓坤,等. 酸性电解水对肉源性荧光假单胞菌的致死效应[J]. 中国农业科学, 2019, 52(9): 1614-1623.
[21]LIU F, WANG F T, DU L H, et al. Antibacterial and antibiofilm activity of phenyllactic acid against Enterobacter cloacae [J]. Food Control, 2018, 84: 442-448.
[22]LIU F, TANG C, WANG D B, et al. The synergistic effects of phenyllactic acid and slightly acid electrolyzed water to effectively inactivate Klebsiella oxytoca planktonic and biofilm cells [J]. Food Control, 2020, 20: 107804.
[23]DUBOIS M, GILLES K A, HANILTON J K, et al. Colorimetric method for determination of sugars and related substances [J]. Anal Chem, 1956, 28: 350.
[24]蔡晓军,孙杨赢,潘道东,等. 迷迭香主要组分对沙门氏菌的抑制机理[J]. 中国食品学报, 2019, 19(3): 134-140.
[25]食品安全国家标准食品微生物学检测菌落总数: GB 4789.2—2016［S］． 北京: 中国标准出版社，2016．
[26]食品安全国家标准食品微生物学检测金黄色葡萄球菌: GB 4789.10—2016［S］. 北京: 中国标准出版社, 2016．
[27]李莎莎,计红芳,张令文,等. 冰温保鲜过程中鸡肉品质及微观结构的变化[J]. 食品与发酵工业, 2019, 45(16): 201-207.
[28]王光宇,李晴,唐文倩,等. nuoB对莓实假单胞菌生理特性及在冷鲜鸡肉中致腐能力的影响[J]. 中国农业科学, 2021,54(8): 1761-1771 .
[29]LI J, SUO Y J, LIAO X Y, et al. Analysis of Staphylococcus aureus cell viability, sublethal injury and death induced by synergistic combination of ultrasound and mild heat [J]. Ultrason Sonochem, 2017, 39: 101-110.
[30]WANG C J, CHANG T, YANG H, et al. Antibacterial mechanism of lactic acid on physiological and morphological properties of Salmonella Enteritidis, Escherichia coli and Listeria monocytogenes [J]. Food Control, 2015, 47：231-236.
[31]OMORI Y, MIAKE K, NAKAMURA H, et al. Influence of lactic acid and post-treatment recovery time on the heat resistance of Listeria monocytogenes [J]. Int J Food Microbiol, 2017，257: 10-18.
[32]许愈,张昭寰,赵莉,等. 应用酸性电解水联合超声波波杀灭副溶血性弧菌[J]. 上海海洋大学学报, 2020, 29(4) : 578-584.
[33]HE Q, ZHANG L J, SONG L Y, et al. Inactivation of Staphylococcus aureus using ultrasound in combination with thyme essential oil nanoemulsions and its synergistic mechanism [J]. LWT - Food Sci Technol, 2021, 147：111574.
[34]王心磊. 超声波杀菌技术对大肠杆菌O157∶H7及其生物膜的杀菌机制研究[D]. 镇江:江苏大学, 2020.
[35]RAMEZANI F, NAJAFI M A, RAHNAMA M, et al. Separate and combined effects of lactic acid, chitosan and modified atmosphere packaging on the shelf life of quail carcass under chilled conditions [J]. Int J Food Microbiol, 2019, 289: 215-222.
[36]GONZLEZ-FANDOS E, MARTINEZ-LAORDEN A, PEREZ-ARNEDO I. Efficacy of combinations of lactic acid and potassium sorbate against Listeria monocytogenes in chicken stored under modified atmospheres [J]. Food Microbiol, 2020, 93: 103596.

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备注/Memo

备注/Memo:
收稿日期：2022-03-23基金项目：国家自然科学基金项目（31871866）；江苏省政策引导类计划（苏北科技专项）(XZ-SZ202012)；江苏省普通高校研究生科研创新计划项目（SJCX20_1428）作者简介：张娇娇（1997-），女，安徽灵璧人，硕士研究生，研究方向为畜禽产品加工与质量控制。(E-mail)1656040130@qq.com 通讯作者：刘芳，(E-mail)fangliu82@163.com
更新日期/Last Update: 2023-01-13