[1]王海波,代冬琴,郭俊云.小桐子14-3-3基因家族的鉴定及低温胁迫应答[J].江苏农业学报,2017,(05):1007-1015.[doi:doi:10.3969/j.issn.1000-4440.2017.05.008]
　WANG Hai-bo,DAI Dong-qin,GUO Jun-yun.Genome-wide identification and chilling stress response of 14-3-3 gene family in Jatropha curcas[J].,2017,(05):1007-1015.[doi:doi:10.3969/j.issn.1000-4440.2017.05.008]
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小桐子14-3-3基因家族的鉴定及低温胁迫应答()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
1007-1015
栏目:
遗传育种·生理生化
出版日期:
2017-10-30

文章信息/Info

Title:
Genome-wide identification and chilling stress response of 14-3-3 gene family in Jatropha curcas
作者:
王海波1; 2; 代冬琴1; 2; 郭俊云1
(1.曲靖师范学院云南高原生物资源保护与利用研究中心/生物资源与食品工程学院，云南曲靖655011；2.曲靖师范学院云南省高校云贵高原动植物遗传多样性及生态适应性重点实验室，云南曲靖655011)
Author(s):
WANG Hai-bo1; 2; DAI Dong-qin1; 2; GUO Jun-yun1
(1.Center for Yunnan Plateau Biological Resources Protection and Utilization/College of Biological Resource and Food Engineering, Qujing Normal University, Qujing 655011， China;2.Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on YunGui Plateau, Qujing Normal University, Qujing 655011， China)
关键词:
小桐子; 14-3-3蛋白; 基因组; 低温胁迫应答
Keywords:
Jatropha curcas; 14-3-3 protein; genome; chilling stress response
分类号:
S794.9
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.008
文献标志码:
A
摘要:
14-3-3蛋白家族在真核生物中广泛存在且高度保守，以同源/异源二聚体形式与2个靶蛋白或1个靶蛋白的两个磷酸化Ser/Thr模体结构域结合来发挥其调控作用。以拟南芥14-3-3蛋白为探针序列，对小桐子蛋白质数据库进行搜索，共鉴定到9个14-3-3基因，其中ε类4个、非ε类5个，并对其理化性质、系统进化、基因结构、顺式作用元件及低温胁迫表达等进行了分析。结果显示，小桐子14-3-3基因在进化上较为保守，存在该家族中典型的9段 α-螺旋结构，ε类基因结构包含7个外显子，非ε类包含 4～5个外显子。另外，在9个14-3-3基因上游调控区都鉴定到多个激素与逆境胁迫应答元件。qRT-PCR分析结果显示，包含有低温应答元件的Jc14-3-3b与Jc14-3-3h基因在小桐子的各组织器官中都有表达，但存在组织表达特异性，都在茎中表达量较高，其次是根，而在叶中表达量相对较低。同时，Jc14-3-3b与Jc14-3-3h基因都属于低温诱导表达基因，分别在茎与根中低温胁迫 0.5～30 h达到最大表达量，与小桐子的抗冷性形成直接相关。本研究为小桐子14-3-3基因家族的克隆与功能验证提供了参考。
Abstract:
The family of 14-3-3 proteins, which acts as homodimers or heterodimers, exists in all eukaryotic organisms with highly conserved sequence. The regulatory function of 14-3-3 proteins is mainly mediated by phosphoerine/phosphothreonine motifs with two targeting proteins or two different domains in one targeting protien simultaneously. Based on the conserved domains of Arabidopsis thaliana 14-3-3 protein as probe sequences, nine 14-3-3 genes including 4 ε-type and 5 non-ε type were identified from the Jatropha curcas protein database, and the physical and chemical characteristics, evolutionary relationship, gene structure, cis-elements, and chilling stress response were systematically analyzed. The result showed that 14-3-3 protein in J. curcas were highly conserved and classical 9-α-helix were found in all 14-3-3 proteins’ secondary structures. Seven exons were included in ε-type gene structure and 4-5 exons were in non-ε type. Multiple cis-elements responsive to different homones and environmental stresses were found in upstream sequences of each 14-3-3 genes in J. curcas. Quantitative RT-PCR revealed that Jc14-3-3b and Jc14-3-3h were expressed differently in different tissues, abundant in stem and root, scarce in leaf. Chilling responsive genes Jc14-3-3b and Jc14-3-3h reached the highest expression level in stem and root after 0.5-30 h chilling stress induction.

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备注/Memo

备注/Memo:
收稿日期：2017-04-02 基金项目：国家自然科学基金项目（31460179） 作者简介：王海波（1980-），男，山西长治人，博士，副教授，研究方向为植物逆境分子生物学。（E-mail）bocai0406@163.com
更新日期/Last Update: 2017-11-03