[1]刘潮,韩利红,宋培兵,等.桑树类甜蛋白家族鉴定与生物信息学分析[J].江苏农业学报,2017,(05):998-1006.[doi:doi:10.3969/j.issn.1000-4440.2017.05.007]
 LIU Chao,HAN Li-hong,SONG Pei-bing,et al.Identification and bioinformatics analysis of thaumatin-like protein family in Morus notabilis[J].,2017,(05):998-1006.[doi:doi:10.3969/j.issn.1000-4440.2017.05.007]
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桑树类甜蛋白家族鉴定与生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
998-1006
栏目:
遗传育种·生理生化
出版日期:
2017-10-30

文章信息/Info

Title:
Identification and bioinformatics analysis of thaumatin-like protein family in Morus notabilis
作者:
刘潮韩利红宋培兵王德琴袁国芳王海波唐利洲
(曲靖师范学院云南高原生物资源保护与利用研究中心/生物资源与食品工程学院/云南省高校云贵高原动植物多样性及生态适应性进化重点实验室,云南曲靖655011)
Author(s):
LIU ChaoHAN Li-hongSONG Pei-bingWANG De-qinYUAN Guo-fangWANG Hai-boTANG Li-zhou
(Center for Yunnan Plateau Biological Resources Protection and Utilization/College of Biological Resource and Food Engineering/Key Laboratory of Yunnan Province Universities of the Diversity and Ecological Adaptive Evolution for Animals and Plants on Yungui Plateau, Qujing Normal University, Qujing 655011, China)
关键词:
桑树类甜蛋白系统进化密码子偏性
Keywords:
Morus notabilisthaumatin-like proteinphylogeny analysiscodon usage bias
分类号:
S888.71
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.007
文献标志码:
A
摘要:
为了全面分析桑树类甜蛋白家族基因特征,从桑树全基因组中鉴定类甜蛋白家族(Thaumatin-like protein,TLP)基因,并对基因结构和组成、蛋白质结构域和系统进化以及密码子使用特性进行分析。通过研究,筛选并鉴定了25个桑树TLP家族成员。多数具有典型的索马甜家族标签和保守氨基酸残基。基因结构分析结果表明,12个TLP基因家族成员含有1个内含子,10个成员含有2个内含子,且内含子相位较保守。系统进化分析结果显示,桑树TLP分为8个聚类组,组内序列一致性较高。密码子偏性分析结果显示,桑树TLP家族基因密码子第3位的核苷酸使用偏性较弱,多数基因的进化主要是由碱基随机突变造成的,属于低表达基因,少数基因受自然选择压力影响,基因表达水平较高,在植物响应环境胁迫中发挥作用。
Abstract:
In order to comprehensively understand the genetic characteristics of thaumatin-like protein (TLP) family, the TLP genes of mulberry whole genome were identified, and the gene structure, protein domain, phylogeny and codon usage bias were analyzed. A total of 25 TLP sequences were identified, most of which have the typical thaumatin sequence and conserved amino acid residues. Each of 12 TLP family members contains one conservative intron, and each of 10 family members contains two conservative introns. The phylogenetic analysis revealed that the TLPs were divided into eight groups, and the sequence similarity was relatively high in each group. Codon usage bias of the third codon of TLP family genes was weak, indicative of the low expression. Base mutation pressure are responsible for the evolution of most of mulberry TLP family genes. Natural selection pressure contributes to a few genes evolution, suggestive of high expression which may play an important role in plant in response to environment stress.

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备注/Memo

备注/Memo:
收稿日期:2017-03-14 基金项目:国家自然科学基金项目(31460179);云南省高校科技创新团队项目 作者简介:刘潮(1980-),男,河北景县人,博士,讲师,主要从事分子植物病理学研究,(E-mail)liuchao@mail.qjnu.edu.cn 通讯作者:唐利洲,(E-mail)tanglizhou@163.com
更新日期/Last Update: 2017-11-03