[1]张添菊,李春阳,吴寒,等.盐蒿提取液抗食源性致病菌活性及其抑菌机理[J].江苏农业学报,2017,(04):932-937.[doi:doi:10.3969/j.issn.1000-4440.2017.04.032]
　ZHANG Tian-ju,LI Chun-yang,WU Han,et al.Antimicrobial activities and mechanism of Artemisia halodendron extract[J].,2017,(04):932-937.[doi:doi:10.3969/j.issn.1000-4440.2017.04.032]
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盐蒿提取液抗食源性致病菌活性及其抑菌机理()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年04期
页码:
932-937
栏目:
加工贮藏·质量安全
出版日期:
2017-08-30

文章信息/Info

Title:
Antimicrobial activities and mechanism of Artemisia halodendron extract
作者:
张添菊1; 李春阳1; 吴寒1; 闫征11; 贾成琦2; 祁海春2
(1.江苏省农业科学院农产品加工研究所，江苏南京210014；2.江苏海苑食品有限公司，江苏滨海224000)
Author(s):
ZHANG Tian-ju1; LI Chun-yang1; WU Han1; YAN Zheng1; JIA Cheng-qi2; QI Hai-chun2
(1.Institute of Agro-product Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Jiangsu Haiyuan Food Limited Company, Binhai 224000, China)
关键词:
盐蒿提取液; 抑菌活性; 抑菌机理
Keywords:
Artemisia halodendron extract; antimicrobial activity; antimicrobial mechanism
分类号:
Q935
DOI:
doi:10.3969/j.issn.1000-4440.2017.04.032
文献标志码:
A
摘要:
以5种常见的致病菌为试验对象，采用体外抑菌试验筛选出盐蒿提取液作用效果最好的致病菌，并通过研究盐蒿提取液对致病菌作用前后细菌培养液的OD260、电导率、碱性磷酸酶(AKP)活性和细胞超微结构的变化，初步探讨盐蒿提取液对单增李斯特菌(Listeria monocytogenes)的抑菌机制。结果显示，盐蒿提取液对5种致病菌均有抑菌活性，但对单增李斯特菌抑制效果最好，最小抑菌浓度（MIC）为0.062 5 mg/ml，对5种致病菌抑制效果表现为：单增李斯特菌＞金黄色葡萄球菌＞大肠杆菌＞肠炎沙门氏菌＞鲍氏志贺氏菌。单增李斯特菌经盐蒿提取液处理后，细菌培养液260 nm的吸光度、电导率和AKP活性均增大，表明盐蒿提取液可破坏单增李斯特菌细胞壁和细胞膜的结构，导致细胞通透性增加，进而使细胞内容物外泄，使细胞生长受到抑制，进而导致死亡。
Abstract:
Five kinds of food-derived pathogenic bacteria were used for the detection of antimicrobial activity of Artemisia halodendron extract, and the antimicrobial mechanisms were studied by determining the change of OD260, electric conductivity and alkaline phosphatase content of the culture solution for bacteria and the cell structure under scanning electron microscope of Listeria monocytogenes. A. halodendron extract exhibited inhibitory effects on Staphylococcus aureus, Escherichia coli, L. monocytogenes, Shigella bogdii and Salmonella. The minimum inhibitory concentration(MIC) against L. monocytogenes was 0.062 5 mg/ml. The antibacterial activity on the bacteria from strong to weak followed the order of L. monocytogene>S. aureus>E. coli>Salmonellai>S. bogdi. The increase of OD260, electric conductivity and alkaline phosphatase content of bacteria culture solution indicated that A. halodendron extract could damage the structure of cell wall and membrane, resulting in the increase of permeability of cell membrane and release of cell components, thereby inhibiting the growth of cell and causing death.

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备注/Memo

备注/Memo:
收稿日期：2017-02-07 基金项目：2015年国家留学基金项目(201508320085)；2014年苏北科技发展计划(富民强县)项目(BN2014147) 作者简介：张添菊(1992-)，女，贵州贵阳人，硕士研究生，主要从事营养与功能食品研究。（Tel）15950572309；(E-mail)1151201245@qq.com 通讯作者：李春阳，（E-mail）lichunyang968@126.com
更新日期/Last Update: 2017-09-01