[1]田晓涵,刘玉玲,李永梅,等.两种不同生境植物棉花与雪莲CDPK1基因的克隆及生物信息学分析[J].江苏农业学报,2016,(05):1005-1012.[doi:10.3969/j.issn.1000-4440.2016.05.008]
　TIAN Xiao-han,LIU Yu-ling,LI Yong-mei,et al.Cloning and bioinformatics analysis of CDPK1 gene in Gossypium hirsutum and Sasussured involucrata from two different habitats[J].,2016,(05):1005-1012.[doi:10.3969/j.issn.1000-4440.2016.05.008]
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两种不同生境植物棉花与雪莲CDPK1基因的克隆及生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年05期
页码:
1005-1012
栏目:
遗传育种·生理生化
出版日期:
2016-11-22

文章信息/Info

Title:
Cloning and bioinformatics analysis of CDPK1 gene in Gossypium hirsutum and Sasussured involucrata from two different habitats
作者:
田晓涵; 刘玉玲; 李永梅; 李锦; 庞学兵; 祝建波; 朱新霞
石河子大学生命科学学院农业生物技术重点实验室, 新疆石河子832000
Author(s):
TIAN Xiao-han; LIU Yu-ling; LI Yong-mei; LI Jin; PANG Xue-bing; ZHU Jian-bo; ZHU Xin-xia
College of Life Science, Shihezi University, Shihezi 832000, China
关键词:
钙依赖性蛋白激酶(CDPK); 生物信息学; 序列分析; 结构预测
Keywords:
calcium-dependent protein kinase (CDPK); bioinformatics; sequence analysis; structure prediction
分类号:
Q785
DOI:
10.3969/j.issn.1000-4440.2016.05.008
文献标志码:
A
摘要:
为了研究两种不同生境植物钙依赖性蛋白激酶基因1（CDPK1）的差异，分别从冷敏感作物陆地棉和高耐寒植物天山雪莲中克隆得到GhCDPK1和SikCDPK1基因。通过生物信息学分析发现，GhCDPK1和SikCDPK1的开放阅读框长度分别为1 764 bp和1 716 bp，各编码587和571个氨基酸。蛋白质表现为弱酸性，且不稳定，亲水；二级结构中以无规则卷曲和α-螺旋为主，三级结构同源建模成功；无跨膜结构域和信号肽，有多个磷酸化位点，但数量存在差异；均具有典型的CDPK保守结构域。GhCDPK1和SikCDPK1氨基酸序列同源性为79.9%，二者明显的区别在于GhCDPK1含有4个EF-hand基序，但SikCDPK1只存在2个EF-hand基序。进化分析结果表明，GhCDPK1与可可CDPK1亲缘关系最近，SikCDPK1与菊花CDPK2亲缘关系最近。
Abstract:
To study the functional differences of calcium-dependent protein kinase gene 1 (CDPK1) in different habitats, 2 calcium-dependent protein kinase genes GhCDPK1 and SikCDPK1 were obtained from coldness-sensitive crop Gossypium hirsutum and hardy plant Sasussured involucrata Kar. et Kir, respectively. GhCDPK1 gene contains a 1 764-bp open reading frame encoding 587 amino acids, and SikCDPK1 contains a 1 716-bp open reading frame encoding 571 amino acids. The corresponding proteins are unstable, hydrophilic and mildly acidic, with random coil and α-helix dominating the secondary structure. Two deduced proteins have no transmembrane region and signal peptide. The protein sequences of GhCDPK1 and SikCDPK1 include multiple phosphorylation sites with different numbers, and contain a typical CDPK conserved domain. The GhCDPK1 and SikCDPK1 shared amino acid sequence homology of 79.9%. There are four conservative EF-hands in GhCDPK1 but only two EF-hands in SikCDPK1. Phylogenic analysis revealed that the GhCDPK1 protein was the closest to Theobroma cacao CDPK1, and the SikCDPK1 protein was the closest to Chrysanthemum boreale CDPK2.

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备注/Memo

备注/Memo:
收稿日期：2016-01-07 基金项目：新疆生产建设兵团项目(2014CC005); 国家自然科学基金项目(31360053); 石河子大学育种专项(gxjs2014-yz04) 作者简介：田晓涵(1990-), 女, 新疆昌吉人, 硕士研究生, 生物化学和分子生物学学专业。（Tel）15609932775; （E-mail）723745781@qq.com 通讯作者：朱新霞, （Tel）13029616626; （E-mail）zhuxxshz@126.com
更新日期/Last Update: 2016-11-22