[1]施雅青,郑耀通.活性氧在黄孢原毛平革菌产木质素过氧化物酶中的调控机制[J].江苏农业学报,2016,(03):514.[doi:10.3969/j.issn.1000-4440.2016.03.005]
　SHI Ya-qing,ZHENG Yao-tong.Regulation mechanism of reactive oxygen species in the lignin peroxidase production of Phanerochaete chrysosporium[J].,2016,(03):514.[doi:10.3969/j.issn.1000-4440.2016.03.005]
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活性氧在黄孢原毛平革菌产木质素过氧化物酶中的调控机制()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
514
栏目:
遗传育种·生理生化
出版日期:
2016-06-30

文章信息/Info

Title:
Regulation mechanism of reactive oxygen species in the lignin peroxidase production of Phanerochaete chrysosporium
作者:
施雅青;  郑耀通
福建农林大学资源与环境学院,福建 福州 350002
Author(s):
SHI Ya-qing;  ZHENG Yao-tong
College of the Resource and Environment, Fujian Agriculture and Forestry University, Fuzhou 350002, China
关键词:
活性氧;  黄孢原毛平革菌;  木质素过氧化物酶;  调控机制
Keywords:
reactive oxygen species;  Phanerochaete chrysosporium;  lignin peroxidase;  regulation mechanism
分类号:
X172
DOI:
10.3969/j.issn.1000-4440.2016.03.005
文献标志码:
A
摘要:
为了探究活性氧对黄孢原毛平革菌产木质素过氧化物酶的调控机制,以黄孢原毛平革菌(Phanerochaete chrysosporium)野生菌株pc530及突变菌株pcR5305为研究对象,研究两菌株在富氮液体培养基及外加不同浓度活性氧供体条件下,羟自由基(·OH)浓度与木质素过氧化物酶(LiP)活性的动态变化规律。结果表明,两菌株均能产生较高浓度的·OH,且·OH浓度与LiP活性变化趋势相一致,但·OH的绝对浓度与LiP的产生量并不相关; 不同浓度的H2O2均能提高两菌株LiP的产生量; 外加二甲亚砜(DMSO)和芬顿试剂(Fenton)均能对两供试菌株合成LiP及产生·OH起作用。因此推测,·OH不直接参与或影响两菌株合成LiP,更可能是作为一种信号分子在起作用。活性氧作为一种胁迫因子对两菌株的产酶具有极为重要的作用。
Abstract:
Phanerochaete chrysosporium wild type pc530 and mutant strain pcR5305 were cultured to study the dynamic changes of hydroxyl radical(·OH)concentration and lignin peroxidase(LiP)activities in nitrogen-rich liquid medium and in the medium added with various concentrations of reactive oxygen species(ROS)donor(H2O2). Both strains produced high concentrations of ·OH, and the change of ·OH concentration was similar to that of LiP. No correlation was found between the absolute concentration of ·OH and the LiP yield. The yield of LiP produced by the two strains was enhanced by H2O2 despite of concentration. Additional DMSO and Fenton influenced LiP synthesis and ·OH production for both strains. The results suggest that ·OH is likely to act as a signalling molecule instead of being directly involved in the synthesis of the LiP, and active oxygen as a stress factor plays an important role in enzyme production by the two strains.

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备注/Memo

备注/Memo:
收稿日期:2015-12-14
作者简介::施雅青(1990-),女,福建晋江人,硕士研究生,研究方向为环境微生物学。(E-mail)471793212@qq.com
通讯作者:郑耀通,(E-mail)zyt5188@aliyun.com
更新日期/Last Update: 2016-06-30