[1]罗光华,宋俊贤,姚 静,等.三化螟两个乙酰胆碱酯酶基因克隆及序列分析[J].江苏农业学报,2016,(03):521.[doi:10.3969/j.issn.1000-4440.2016.03.006]
　LUO Guang-hua,SONG Jun-xian,YAO Jing,et al.Cloning and molecular characterization of two acetylcholinesterase genes from Scirpophaga incertulas(Lepidoptera: Pyralidae)[J].,2016,(03):521.[doi:10.3969/j.issn.1000-4440.2016.03.006]
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三化螟两个乙酰胆碱酯酶基因克隆及序列分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
521
栏目:
植物保护
出版日期:
2016-06-30

文章信息/Info

Title:
Cloning and molecular characterization of two acetylcholinesterase genes from Scirpophaga incertulas(Lepidoptera: Pyralidae)
作者:
罗光华1;  宋俊贤2;  姚 静1;  郑少秋1;  方继朝1
1.江苏省农业科学院植物保护研究所,江苏 南京 210014; 2.江苏省溧阳市植保植检站,江苏 溧阳 213300
Author(s):
LUO Guang-hua1;  SONG Jun-xian2;  YAO Jing1;  ZHENG Shao-qiu1;  FANG Ji-chao1
1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2.Liyang Plant Protection and Quarantine Station of Jiangsu, Liyang 213300, China
关键词:
三化螟;  乙酰胆碱酯酶基因;  克隆
Keywords:
Scirpophaga incertulas;  acetylcholinesterase;  cloning
分类号:
S435.112+.1
DOI:
10.3969/j.issn.1000-4440.2016.03.006
文献标志码:
A
摘要:
乙酰胆碱酯酶(AChE)是有机磷(Organophosphate,OP)和氨基甲酸酯(Carbamate,CB)类杀虫剂的作用靶标。昆虫乙酰胆碱酯酶基因(ace)表达量的上升或基因突变导致其对杀虫剂的敏感性下降是昆虫产生抗药性的重要原因。本研究利用RT-PCR技术和cDNA末端快速扩增(RACE)技术成功克隆到三化螟(Scirpophaga incertulas Walker)的两个乙酰胆碱酯酶基因,分别命名为Siace1和Siace2。Siace1的完整开放阅读框(Open reading frame,ORF)长2 085 bp,编码含694个氨基酸的乙酰胆碱酯酶1(SiAChE1)。Siace2的ORF长1 917 bp,编码含638个氨基酸的乙酰胆碱酯酶2(SiAChE2)。将三化螟的两个乙酰胆碱酯酶与已报道的各物种的乙酰胆碱酯酶进行相似性比较,结果显示在蛋白水平上,SiAChE1与台湾稻螟的AChE1有最高的相似性,达到94%; SiAChE2与二化螟的AChE2具有最高的相似性,为98%。本研究为水稻上重要螟虫的乙酰胆碱酯酶基因对比研究提供了基础。
Abstract:
Acetylcholinesterase(AChE)is the target of organophosphate(OP)and carbamate(CB)insecticides. Mutations in the AChE gene(ace)leading to decreased insecticide susceptibility is responsible for decreased insecticides resistance in insects. In this study, two Scirpophaga incertulas Walker acetylcholinesterase genes, Siace1 and Siace2, were cloned using RT-PCR and RACE. The open reading frame(ORF)of Siace1 is 2 085 bp in length, encoding acetylcholinesterase 1(SiAChE1)protein containing a calculated 694 amino acid(aa)residues. Siace2 is 1 917 bp in length, encoding an acetylcholinesterase 2(SiAChE2)containing 638 aa residues. At the aa level, SiAChE1 shares the highest similarity(94%)with the Chilo auricilius AChE1, and SiAChE2 shares the highest similarity with the C. suppressalis AChE2(98%). This study provides a basis for future comparison of ace genes in different pyralididae insects in rice.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-10-15
基金项目:公益性行业(农业)科研专项(201303017); 国家自然科学基金项目(31201505); 江苏省农业科技自主创新基金项目[CX(15)1055]
作者简介::罗光华(1982-),男,江苏溧阳人,博士,副研究员,主要从事农业害虫综合防控研究。(Tel)025-84390172;(E-mail)ghluo@jaas.ac.cn。宋俊贤为共同第一作者。
通讯作者:方继朝,(E-mail)fangjc@jaas.ac.cn
更新日期/Last Update: 2016-06-30