[1]包国媛,李文英,张慧敏,等.基于转录组学的新西兰甜菜干旱胁迫响应分子机制与关键基因发掘[J].江苏农业学报,2026,42(07):1447-1455.[doi:doi:10.3969/j.issn.1000-4440.2026.07.016]
　BAO Guoyuan,LI Wenying,ZHANG Huimin,et al.Transcriptome-based analysis on molecular mechanisms and key gene identification of New Zealand beet in response to drought stress[J].,2026,42(07):1447-1455.[doi:doi:10.3969/j.issn.1000-4440.2026.07.016]
点击复制

基于转录组学的新西兰甜菜干旱胁迫响应分子机制与关键基因发掘()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年07期
页码:
1447-1455
栏目:
园艺
出版日期:
2026-07-31

文章信息/Info

Title:
Transcriptome-based analysis on molecular mechanisms and key gene identification of New Zealand beet in response to drought stress
作者:
包国媛1; 李文英1; 张慧敏1; 2; 3; 4; 王雅琼1; 2; 3; 4
(1.青海民族大学生态环境与资源学院,青海西宁810007；2.青海省特色经济植物高值化利用重点实验室,青海西宁810007；3.生物技术与分析测试实验室,青海西宁810007；4.青藏高原资源化学与生态环境保护国家民委重点实验室,青海西宁810007)
Author(s):
BAO Guoyuan1; LI Wenying1; ZHANG Huimin1; 2; 3; 4; WANG Yaqiong1; 2; 3; 4
(1.College of Ecological Environment and Resources, Qinghai Minzu University, Xining 810007, China；2.Key Laboratory of High-value Utilization of Characteristic Economic Plants in Qinghai Province, Xining 810007, China；3.Laboratory of Biotechnology and Analytical Testing, Xining 810007, China；4.State Ethnic Affairs Commission Key Laboratory of Resource Chemistry and Ecological Environment Protection on the Qinghai-Tibet Plateau, Xining 810007, China)
关键词:
甜菜; 干旱胁迫; 转录组分析; 差异表达基因
Keywords:
Beta vulgaris L.; drought stress; transcriptome analysis; differentially expressed genes
分类号:
S651
DOI:
doi:10.3969/j.issn.1000-4440.2026.07.016
文献标志码:
A
摘要:
本研究以新西兰甜菜作为试验材料,探究其响应干旱胁迫的分子机制。转录组分析与代谢通路富集分析结果表明,在干旱胁迫下,甜菜差异表达基因富集于植物激素信号转导、次生代谢产物合成、MAPK信号、甜菜碱合成和苯丙烷类合成等通路中。筛选出甘氨酸甜菜碱合成途径中的3个关键酶基因,分别为甜菜碱醛脱氢酶(BADH)基因、胆碱单加氧酶(CMO)基因和磷脂酰乙醇胺N-甲基转移酶(PEAMT)基因。同时鉴定到多个调控甜菜碱合成的转录因子基因,包括编码AP2、bZIP、DBB、Dof、ERF、G2-like、HD-ZIP、MYB和NAC等转录因子的基因。随着干旱胁迫处理时间的延长,LOC104888974(编码AP2转录因子)、LOC104883107(编码bZIP转录因子)、LOC104888829(编码DBB转录因子)、LOC104904254(编码DBB转录因子)、LOC104906222(编码Dof转录因子)、LOC104896121(编码ERF转录因子)、LOC104908699(编码G2-like转录因子)、LOC104896116(编码HD-ZIP转录因子)、LOC104905523(编码HD-ZIP转录因子)、LOC104895550(编码MYB转录因子)、LOC104901408(编码MYB转录因子)、LOC104891267(编码MYB-related转录因子)和 LOC104907290(编码NAC转录因子)基因的表达量呈上升趋势,表明这些基因可能在干旱胁迫响应过程中发挥重要作用。本研究结果为解析新西兰甜菜抗旱机制及抗旱育种提供了理论依据。
Abstract:
In this study, New Zealand beet was used as experimental material to explore its molecular mechanisms underlying drought stress response. Transcriptome analysis and metabolic pathway enrichment analysis revealed that differentially expressed genes (DEGs) of beet under drought stress were significantly enriched in multiple pathways, including plant hormone signal transduction, secondary metabolite biosynthesis, MAPK signaling pathway, betaine biosynthesis and phenylpropanoid biosynthesis. Three key enzyme genes involved in the glycine betaine synthesis pathway were identified, namely betaine aldehyde dehydrogenase (BADH) gene, choline monooxygenase (CMO) gene and phosphoethanolamine N-methyltransferase (PEAMT) gene. Meanwhile, numerous transcription factor genes regulating betaine synthesis were identified, including those encoding AP2, bZIP, DBB, Dof, ERF, G2-like, HD-ZIP, MYB and NAC. With the prolongation of drought stress duration, the expression levels of multiple genes showed an upward trend, including LOC104888974 (encoding AP2 transcription factor), LOC104883107 (encoding bZIP transcription factor), LOC104888829 (encoding DBB transcription factor), LOC104904254 (encoding DBB transcription factor), LOC104906222 (encoding Dof transcription factor), LOC104896121 (encoding ERF transcription factor), LOC104908699 (encoding G2-like transcription factor), LOC104896116 (encoding HD-ZIP transcription factor), LOC104905523 (encoding HD-ZIP transcription factor), LOC104895550 (encoding MYB transcription factor), LOC104901408 (encoding MYB transcription factor), LOC104891267 (encoding MYB-related transcription factor), and LOC104907290 (encoding NAC transcription factor). These results suggested that these genes may play crucial roles in drought stress response. This study provides a theoretical basis for elucidating the drought resistance mechanism and facilitating drought-resistant breeding of New Zealand beet.

参考文献/References:

[1]Chhikara N, Kushwaha K, Sharma P, et al. Bioactive compounds of beetroot and utilization in food processing industry:a critical review[J]. Food Chemistry,2019,272:192-200.
[2]李梦杰,王志青,仝 涛. 甜菜根关键活性成分及其生物功能研究进展[J]. 食品安全质量检测学报,2022,13(2):343-350.
[3]Lidder S, Webb A J. Vascular effects of dietary nitrate (as found in green leafy vegetables and beetroot) via the nitrate-nitrite-nitric oxide pathway[J]. British Journal of Clinical Pharmacology,2013,75(3):677-696.
[4]Guldiken B, Toydemir G, Nur Memis K, et al. Home-processed red beetroot (Beta vulgaris L.) products: changes in antioxidant properties and bioaccessibility[J]. International Journal of Molecular Sciences,2016,17(6):858.
[5]Ninfali P, Angelino D. Nutritional and functional potential of Beta vulgaris cicla and rubra[J]. Fitoterapia,2013,89:188-199.
[6]Kathiravan T, Nadanasabapathi S, Kumar R. Standardization of process condition in batch thermal pasteurization and its effect on antioxidant,pigment andmicrobial inactivation of Ready to Drink (Rtd) beetroot (Beta vulgaris L.) juice[J]. International Food Research Journal,2014,21(4):1305-1312.
[7]Clifford T, Howatson G, West D J, et al. The potential benefifits of red beetroot supplementation in health and disease[J]. Nutrients,2015,7(4):2801-2822.
[8]石杨,尹希龙,李王胜,等. PEG模拟干旱胁迫对耐旱型与干旱敏感型甜菜种质形态指标的影响[J]. 中国农学通报,2022,38(29):45-51.
[9]邹利茹,张福顺,刘乃新,等. 甜菜干旱胁迫响应研究进展[J]. 中国糖料,2021,43(1):36-44.
[10]徐晓阳,李国龙,孙亚卿,等. 甜菜NAC转录因子鉴定及其在水分胁迫下的表达分析[J]. 植物生理学报,2019,55(4):444-456.
[11]Kim D, Langmead B, Salzberg S L. HISAT:a fast spliced aligner with low memory requirements[J]. Nature Methods,2015,12(4):357-360.
[12]李小冬,王小利,王茜,等. 干旱胁迫下高羊茅叶片的代谢组学分析[J]. 中国草地学报,2016,38(5):59-65.
[13]Monteiro De Paula F, Pham Thi A T, Zuily-Fodil Y, et al. Effects of water stress on the biosynthesis and degradation of polyunsatura-ted lipid molecular species in leaves of Vigna unguiculata[J]. Plant Physiology and Biochemistry,1993,31(5):707-715.
[14]Matos A R, D′Arcy-Lameta A, Frana M, et al. A novel patatin-like gene stimulated by drought stress encodes a galactolipid acyl hydrolase[J]. Febs Letters,2001,491(3):188-192.
[15]李慧楠,何芳练,邱祖杨,等. 干旱胁迫下芋叶片转录组分析(英文)[J]. 南方农业学报,2025,56(4):1056-1069.
[16]徐兴源,李艳肖,朱梦洋,等. 蓖麻RcbZIP44基因克隆及表达特性[J]. 南方农业学报,2024,55(7):2069-2079.
[17]赵双,尤伟忠,汪溢,等. 基于转录组分析枇杷响应干旱胁迫的分子机制[J]. 江苏农业科学,2024,52(17):27-34.
[18]郭慧慧,潘祥华,蒋元斌,等. 甘薯耐盐性研究进展[J]. 江苏农业科学,2024,52(8):23-31.
[19]Saibo N J M, Lourenco T, Oliveira M M. Transcription factors and regulation of photosynthetic and related metabolism under environmental stresses[J]. Annals of Botany,2009,103(4):609-623.
[20]Rakocevic M, Müller M, Matsunaga F T, et al. Daily heliotropic movements assist gas exchange and productive responses in DREB 1A soybean plants under drought stress in the greenhouse[J]. The Plant Journal,2018,96(4):801-814.
[21]李跃,李国瑞,黄凤兰,等. Dof转录因子研究进展[J]. 安徽农业科学,2016,44(7):96-97.
[22]Puranik S, Sahu P P, Srivastava P S, et al. NAC proteins:regulation and role in stress tolerance[J]. Trends in Plant Science,2012,17(6):369-381.

相似文献/References:

[1]佚名 佚名 佚名.三才期刊采编系统文章正在整理中…[J].江苏农业学报,2005,(01):5.
　XIE Xin,ZHAO Zhong.三才期刊采编系统文章正在整理中…[J].,2005,(07):5.
[2]佚名 佚名 佚名.三才期刊采编系统文章正在整理中…[J].江苏农业学报,2006,(01):5.
　XIE Xin,ZHAO Zhong.三才期刊采编系统文章正在整理中…[J].,2006,(07):5.
[3]熊洁,邹晓芬,邹小云,等.干旱胁迫对不同基因型油菜农艺性状和产量的影响[J].江苏农业学报,2015,(03):494.[doi:10.3969/j.issn.1000-4440.2015.03.005]
　XIONG Jie,ZOU Xiao-fen,ZOU Xiao-yun,et al.Effects of drought stress on agronomic traits and yield of different rapeseed genotypes[J].,2015,(07):494.[doi:10.3969/j.issn.1000-4440.2015.03.005]
[4]肇莹,杨镇,杨涛,等.植物内生菌醇提取物对草坪草抗旱性的影响[J].江苏农业学报,2015,(01):39.[doi:10.3969/j.issn.1000-4440.2015.01.006]
　ZHAO Ying,YANG Zhen,YANG Tao,et al.Influence of plant endophyte extract on turfgrass drought tolerance[J].,2015,(07):39.[doi:10.3969/j.issn.1000-4440.2015.01.006]
[5]孟力力,张俊,闻婧.干旱胁迫对彩叶草光合特性及叶片超微结构的影响[J].江苏农业学报,2015,(01):180.[doi:10.3969/j.issn.1000-4440.2015.01.028]
　MENG Li-li,ZHANG Jun,WEN Jing.Changes of photosynthetic characteristics of Coleus blumei and mesophyll cell ultrastructure in response to drought stress[J].,2015,(07):180.[doi:10.3969/j.issn.1000-4440.2015.01.028]
[6]张丽丽,徐碧玉,刘菊华,等.MaASR1基因通过乙烯途径提高拟南芥抗旱性的作用机制[J].江苏农业学报,2018,(03):511.[doi:doi:10.3969/j.issn.1000-4440.2018.03.005]
　ZHANG Li-li,XU Bi-yu,LIU Ju-hua,et al.The regulation mechanism of MaASR1 gene for improving the drought resistance of Arabidopsis by ethylene pathway[J].,2018,(07):511.[doi:doi:10.3969/j.issn.1000-4440.2018.03.005]
[7]麻云霞,李钢铁,张宏武,等.外源硅对酸枣生长和生理生化特征的影响[J].江苏农业学报,2018,(05):1113.[doi:doi:10.3969/j.issn.1000-4440.2018.05.021]
　MA Yun-xia,LI Gang-tie,ZHANG Hong-wu,et al.Effects of exogenous silicon on growth， physiological and biochemical characteristics of zizyphus jujube plant[J].,2018,(07):1113.[doi:doi:10.3969/j.issn.1000-4440.2018.05.021]
[8]葛道阔,曹宏鑫,杨余旺,等.干旱胁迫下油菜栽培模拟优化决策系统（Rape-CSODS）的订正及其检验[J].江苏农业学报,2019,(01):56.[doi:doi:10.3969/j.issn.1000-4440.2019.01.008]
　GE Dao-kuo,CAO Hong-xin,YANG Yu-wang,et al.Modification and verification of Rape-CSODS under drought stress[J].,2019,(07):56.[doi:doi:10.3969/j.issn.1000-4440.2019.01.008]
[9]陈丽,焦健,朱绍丹,等.油橄榄对牧草间作与干旱胁迫交互作用的根系生理响应[J].江苏农业学报,2019,(06):1434.[doi:doi:10.3969/j.issn.1000-4440.2019.06.024]
　CHEN Li,JIAO Jian,ZHU Shao-dan,et al.Root physiological response of olive to the interaction of pasture intercropping and drought stress[J].,2019,(07):1434.[doi:doi:10.3969/j.issn.1000-4440.2019.06.024]
[10]陈丽,焦健,朱绍丹,等.牧草间作对干旱胁迫下油橄榄根系形态特征的影响[J].江苏农业学报,2020,(01):39.[doi:doi:10.3969/j.issn.1000-4440.2020.01.006]
　CHEN Li,JIAO Jian,ZHU Shao-dan,et al.Effects of pasture intercropping on root morphological characteristics of olive under drought stress[J].,2020,(07):39.[doi:doi:10.3969/j.issn.1000-4440.2020.01.006]

备注/Memo

备注/Memo:
收稿日期：2025-06-25基金项目：青海民族大学2025年大学生创新创业训练计划项目(DCXM-2025-107)作者简介：包国媛(1997-),女,青海互助人,硕士,研究方向为植物资源开发与利用。(E-mail)1791564133@qq.com通讯作者：王雅琼,(E-mail)wangyaqiong727@163.com
更新日期/Last Update: 2026-08-21