[1]刘洋,陈孝月,白裔凡,等.小麦TaXI-III互作蛋白的筛选及其与TaHRLI-2B的互作分析[J].江苏农业学报,2026,42(07):1326-1337.[doi:doi:10.3969/j.issn.1000-4440.2026.07.004]
　LIU Yang,CHEN Xiaoyue,BAI Yifan,et al.Screening for TaXI-III interacting proteins in wheat and analysis of its interaction with TaHRLI-2B[J].,2026,42(07):1326-1337.[doi:doi:10.3969/j.issn.1000-4440.2026.07.004]
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小麦TaXI-III互作蛋白的筛选及其与TaHRLI-2B的互作分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年07期
页码:
1326-1337
栏目:
遗传育种·生理生化
出版日期:
2026-07-31

文章信息/Info

Title:
Screening for TaXI-III interacting proteins in wheat and analysis of its interaction with TaHRLI-2B
作者:
刘洋1; 陈孝月1; 白裔凡1; 陈微1; 司红起2; 蔡华1
(1.滁州学院现代农业与食品工程学院,安徽滁州239000；2.安徽农业大学农学院,安徽合肥230000)
Author(s):
LIU Yang1; CHEN Xiaoyue1; BAI Yifan1; CHEN Wei1; SI Hongqi2; CAI Hua1
(1.School of Modern Agriculture and Food Engineering, Chuzhou University, Chuzhou 239000, China;2.School of Agronomy, Anhui Agricultural University, Hefei 230000, China)
关键词:
小麦; 木聚糖酶抑制剂TaXI-III; 分裂泛素酵母双杂交系统; 互作蛋白; 超敏反应（HR）样病变诱导蛋白TaHRLI-2B
Keywords:
wheat; xylanase inhibitor TaXI-III; split-ubiquitin yeast two-hybrid system; interacting proteins; HR-like lesion-inducing protein TaHRLI-2B
分类号:
S512.1;S330
DOI:
doi:10.3969/j.issn.1000-4440.2026.07.004
文献标志码:
A
摘要:
为探究木聚糖酶抑制剂基因TaXI-III在小麦抵御禾谷镰刀菌侵染中的分子机制,本研究从小麦品种安农1589中克隆出TaXI-III基因,生物信息学与亚细胞定位分析结果显示,TaXI-III蛋白定位于细胞膜/细胞壁。通过同源重组法构建诱饵载体,自激活与功能检测结果显示,pBT3N-TaXI-III在NMY51酵母细胞内无自激活活性,且可正常表达功能蛋白。利用分裂泛素酵母双杂交系统筛选获得19个TaXI-III互作候选蛋白,功能涉及信号转导、跨膜运输、细胞程序性死亡及胁迫响应等。进一步通过酵母回转、荧光素酶互补及双分子荧光互补试验,证实TaXI-III蛋白与超敏反应（HR）样病变诱导蛋白TaHRLI-2B在酵母和植物细胞中互作。逆转录定量实时聚合酶链式反应(RT-qPCR)结果表明,TaHRLI-2B基因受禾谷镰刀菌诱导表达,在拟南芥中过表达TaHRLI-2B基因可增强植株抗病性。本研究结果为解析TaXI-III基因功能机制及小麦抗病基因挖掘提供了理论依据。
Abstract:
In order to further explore the molecular mechanism of the wheat xylanase inhibitor gene TaXI-III in resistance to Fusarium graminearum, this study cloned the TaXI-III gene from the moderately resistant variety Annong 1589. Bioinformatics and subcellular localization analysis revealed that the TaXI-III protein was localized to the cell membrane/wall. The bait vector was constructed by homologous recombination method. The self-activation and functional assays confirmed that pBT3N-TaXI-III had no self-activation activity in NMY51 yeast cells, and could produce functional proteins. A total of 19 candidate proteins that may interact with TaXI-III were finally obtained by split-ubiquitin yeast two-hybrid system from wheat cDNA library. These proteins were associated with diverse biological functions, including signal transduction, transmembrane transport, programmed cell death, and stress response. Results from yeast retransformation, luciferase complementation assay and bimolecular fluorescence complementation assay confirmed that TaHRLI-2B, an HR-like lesion-inducing protein involved in the hypersensitive response, interacted with TaXI-III in both yeast and plant cells. The expression of TaHRLI-2B was induced by Fusarium graminearum infection through quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) analysis. And the overexpression of TaHRLI-2B in Arabidopsis thaliana enhanced the resistance of plants to pathogens. The results of this study provide a theoretical foundation for further exploring the functional mechanism of TaXI-III and discovering disease-related genes in wheat.

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备注/Memo

备注/Memo:
收稿日期：2025-09-23基金项目：安徽省高校自然科学研究重点项目(2022AH051088)；滁州学院科研启动基金项目(2022qd50)；安徽省高校自然科学研究重大项目(2023AH040223)；国家产业技术体系——小麦体系项目(CARS-03)作者简介：刘洋(1991-),男,安徽宿州人,博士,讲师,研究方向为小麦抗病抗逆基因挖掘。(E-mail)97064987@qq.com通讯作者：蔡华,(E-mail)chczh@163.com
更新日期/Last Update: 2026-08-21