参考文献/References:
[1]邹学校,朱凡. 辣椒的起源、进化与栽培历史[J]. 园艺学报,2022,49(6):1371-1381.
[2]王立浩,张宝玺,张正海,等. “十三五”我国辣椒育种研究进展、产业现状及展望[J]. 中国蔬菜,2021(2):21-29.
[3]HYDE K D, CAI L, MCKENZIE E H C, et al. Colletotrichum:a catalogue of confusion[J]. Fungal Diversity,2009,39(1):1-17.
[4]O′CONNELL R J, THON M R, HACQUARD S, et al. Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses[J]. Nature Genetics,2012,44(9):1060-1065.
[5]周黛媛,张正海,曹亚从,等. 辣椒抗炭疽病遗传育种研究进展[J]. 中国蔬菜,2022(2):17-24.
[6]MONGKOLPORN O, TAYLOR P W J. Chili anthracnose:Colletotrichum taxonomy and pathogenicity[J]. Plant Pathology,2018,67(6):1255-1263.
[7]SREENIVASAPRASAD S, BROWN A E, MILLS P R. DNA sequence variation and interrelationships among Colletotrichum species causing strawberry anthracnose[J]. Physiological and Molecular Plant Pathology,1992,41(4):265-281.
[8]MILLS P R, HODSON A, BROWN A E. Molecular differentiation of Colletotrichum gloeosporioides isolates infecting tropical fruits[J]. Brazilian Archives of Biology and Technology,1992,54(6):1099-1108.
[9]CAI L, HYDE K D, TAYLOR P W J, et al. A polyphasic approach for studying Colletotrichum[J]. Fungal Diversity,2009,39(1):183-204.
[10]CANNON P F, DAMM U, JOHNSTON P R, et al. Colletotrichum:current status and future directions[J]. Studies in Mycology,2012,73(1):181-213.
[11]MARIN-FELIX Y, GROENEWALD J Z, CAI L, et al. Genera of phytopathogenic fungi:GOPHY1[J]. Studies in Mycology,2017,86:99-216.
[12]THAN P P, JEEWON R, HYDE K D, et al. Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand[J]. Plant Pathology,2008,57(3):562-572.
[13]MONTRI P, TAYLOR P W J, MONGKOLPORN O. Pathotypes of Colletotrichum capsici,the causal agent of chili anthracnose,in Thailand[J]. Plant Disease,2009,93(1):17-20.
[14]MONGKOLPORN O, MONTRI P, SUPAKAEW T, et al. Differential reactions on mature green and ripe chili fruit infected by three Colletotrichum spp.[J]. Plant Disease,2010,94(3):306-310.
[15]DAMM U, WOUDENBERG J H C, CANNON P F, et al. Colletotrichum species with curved conidia from herbaceous hosts[J]. Fungal Diversity,2009,39:45-87.
[16]RANATHUNGE N P, MONGKOLPORN O, FORD R, et al. Colletotrichum truncatum pathosystem on Capsicum spp.:infection, colonization and defence mechanisms[J]. Australasian Plant Pathology,2012,41:463-473.
[17]DAMM U, CANNON P F, CROUS P W. Colletotrichum:complex species or species complexes?[M]. Utrecht:CBS-KNAW Fungal Biodiversity Centre,2012.
[18]DE SILVA D D, ADES P K, CROUS P W, et al. Colletotrichum species associated with chili anthracnose in Australia[J]. Plant Pathology,2017,66(2):254-267.
[19]DE ARAUJO M S B, SUDR C P, DA SILVA ALENCAR A A, et al. The state-of-the-art in the genetics of resistance to the Colletotrichum species complex in Capsicum[J]. Revisao Anual de Patologia de Plantas,2023,29:7-34.
[20]NISA A U N, WANI A H, MALIK W S, et al. A new report of Colletotrichum jasminigenum,an anthracnose rot causing pathogen to Capsicum annuum in Kashmir valley, India[J]. Journal of Mycopathological Research,2023,61(4):587-591.
[21]SHARMA G, MAYMON M, ELAZAR M, et al. First report of Colletotrichum aenigma and C. perseae causing anthracnose disease on Capsicum annuum in Israel[J]. Crop Protection,2022,152:105853.
[22]ZHANG Y Y, ZHU Z S, XU Y T, et al. First report of Colletotrichum jiangxiense causing anthracnose on chili in Yunnan Province,China[J]. Plant Diease,2023,107(2):568.
[23]DE OLIVEIRA C V S, MATOS K S, ALBUQUERQUE D M C, et al. Identification of Colletotrichum isolates from Capsicum chinense in Amazon[J]. Genetics and Molecular Research,2017,16(2):16029601.
[24]DIAO Y Z, ZHANG C, LIU F, et al. Colletotrichum species causing anthracnose disease of chili in China[J]. Persoonia-Molecular Phylogeny Evolution of Fungi,2017,38(1):20-37.
[25]SHARMA G, PINNAKA A K, SHENOY B D, et al. Infra-specific diversity of Colletotrichum truncatum associated with chilli anthracnose in India based on microsatellite marker analysis[J]. Archives of Phytopathology Plant Protection,2014,47(20):2509-2523.
[26]VOORRIPS R E, FINKERS R, SANJAYA L, et al. QTL mapping of anthracnose (Colletotrichum spp.) resistance in a cross between Capsicum annuum and C. chinense[J]. Theoretical Applied Genetics,2004,109:1275-1282.
[27]KIM K D, OH B J, YANG J M. Differential interactions of a Colletotrichum gloeosporioides isolate with green and red pepper fruits[J]. Phytoparasitica,1999,27:97-106.
[28]NOOR N M, ZAKARIA L. Identification and characterization of Colletotrichum spp. associated with chili anthracnose in peninsular Malaysia[J]. European Journal of Plant Pathology,2018,151:961-973.
[29]HARP T L, PERNEZNY K, IVEY M L L, et al. The etiology of recent pepper anthracnose outbreaks in Florida[J]. Crop Protection,2008,27(10):1380-1384.
[30]KIM K H, YOON J B, PARK H G, et al. Structural modifications and programmed cell death of chili pepper fruit related to resistance responses to Colletotrichum gloeosporioides infection[J]. Phytopathology,2004,94(12):1295-1304.
[31]THAN P P, PRIHASTUTI H, PHOULIVONG S, et al. Chilli anthracnose disease caused by Colletotrichum species[J]. Journal of Zhejiang University Science B,2008,9:764-778.
[32]SILVA S A M, RODRIGUES R, GONCALVES L S A, et al. Resistance in Capsicum spp. to anthracnose affected by different stages of fruit development during pre-and post-harvest[J]. Tropical Plant Pathology,2014,39:335-341.
[33]ALMEIDA L B, MATOS K S, ASSIS L A G, et al. First report of anthracnose of Capsicum chinense in Brazil caused by Colletotrichum brevisporum[J]. Plant Disease,2017,101(6):1035.
[34]RIDZUAN R, RAFII M Y, ISMAIL S I, et al. Breeding for anthracnose disease resistance in chili:progress and prospects[J]. International Journal of Molecular Sciences,2018,19(10):3122.
[35]易兰. 辣椒炭疽病病原菌的分离鉴定和辣椒抗炭疽病材料的筛选[D]. 成都:四川农业大学,2019.
[36]DE SILVA D D, CROUS P W, ADES P K, et al. Life styles of Colletotrichum species and implications for plant biosecurity[J]. Fungal Biology Reviews,2017,31(3):155-168.
[37]GOMES S, BACELAR E, MARTINS-LOPES P, et al. Infection process of olive fruits by Colletotrichum acutatum and the protective role of the cuticle and epidermis[J]. Journal of Agricultural Science,2012,4(2):101-110.
[38]AUYONG A S M, FORD R, TAYLOR P W J, et al. The role of cutinase and its impact on the pathogenicity of Colletotrichum truncatum[J]. Plant Pathology & Microbiology,2015,6(3):1000259.
[39]AUYONG A S M, FORD R, TAYLOR P W J. Genetic transformation of Colletotrichum truncatum associated with anthracnose disease of chili by random insertional mutagenesis[J]. Journal of Basic Microbiology,2012,52(4):372-382.
[40]毛晓瑞,吴飞,刘亚琴. 辣椒炭疽病的发病原因及综合防治对策[J]. 河南农业,2020(19):43-44.
[41]姚攸世,汤春忠. 江苏金湖县辣椒炭疽病发病原因及综合防治措施[J]. 农业工程技术,2021,41(29):42-44.
[42]陈凌敏,孙新纹. 16%吡唑醚菌酯·二氰蒽醌悬浮剂防治辣椒炭疽病田间药效试验[J]. 农业科技与信息,2018(5):10-11.
[43]高杨杨,禾丽菲,李北兴,等. 山东省辣椒炭疽病病原菌的鉴定及高效防治药剂的筛选[J]. 中国农业科学,2017,50(8):1452-1464.
[44]王妮,尹显慧,彭丽娟,等. 辣椒炭疽病病原鉴定及其杀菌剂毒力测定[J]. 植物保护,2019,45(4):216-223.
[45]张河庆,宋占峰,韩帅,等. 四川辣椒炭疽病菌鉴定及防治药剂筛选研究[J]. 中国蔬菜,2024(4):93-99.
[46]王培先,李正男,张磊,等. 辣椒种质抗炭疽病水平鉴定及炭疽病菌药剂敏感性分析[J]. 北方园艺,2022(21):1-8.
[47]王爱萍,席淑文,张国福,等. 苯并烯氟菌唑对尖孢炭疽菌的毒力及对其所致辣椒炭疽病的防效[J]. 植物保护学报,2023,50(3):830-838.
[48]KIM J D, JEON B J, HAN J W, et al. Evaluation of the endophytic nature of Bacillus amyloliquefaciens strain GYL4 and its efficacy in the control of anthracnose[J]. Pest Management Science,2016,72(8):1529-1536.
[49]李叶彤. 辣椒胶孢炭疽菌生物学特性及其拮抗木霉菌筛选[D]. 长春:吉林农业大学,2023.
[50]THANH T L, CONG V K, SANGPUEAK R, et al. Efficacy of Bacillus subtilis for controlling anthracnose in chilli[J]. Agriculture and Natural Resources,2023,57(2):223-232.
[51]谢慧,刘安,陈亮,等. 辐射诱变贝莱斯芽孢杆菌及其对辣椒炭疽病抑菌效果[J]. 辐射研究与辐射工艺学报,2023,41(3):93-103.
[52]宁俊琪. 辣椒内生细菌LY7与咪鲜胺协同防治辣椒炭疽病研究[D]. 晋中:山西农业大学,2022.
[53]PUTRA F, CARSONO N, WIDIANTINI F, et al. Breeding methods for antrachnose resistant chili pepper in the last decade:a review[J]. Agrosainstek Jurnal Ilmu Dan Teknologi Pertanian,2024,8(2):76-90.
[54]卢鉴植,刘建华,巩振辉. 辣椒炭疽病苗期抗病性鉴定方法研究[J]. 江苏农业科学,1992(5):42-44.
[55]巩振辉,王鸣,周新民,等. 辣椒炭疽病病原菌及致病力差异[J]. 北方园艺,1992(1):4-7.
[56]林清,吕中华,黄任中,等. 辣椒炭疽病抗性鉴定方法研究[J]. 西南农业学报,2006,19(6):1071-1073.
[57]毛爱军,胡洽,耿三省. 辣椒炭疽病抗病性鉴定技术及利用[J]. 华北农学报,2004,19(2):87-91.
[58]郑婉婉,赵建荣,程春园,等. 辣椒种质资源炭疽病抗性鉴定与遗传分析[J]. 中国蔬菜,2024(5):59-68.
[59]李洋. 辣椒CaWRKY20和CaWRKY50响应炭疽菌侵染的机理研究及抗炭疽病QTLs定位[D]. 杨凌:西北农林科技大学,2023.
[60]LI Y, MA X, GAI W X, et al. First report of Colletotrichum gloeosporioides causing anthracnose on pepper in Shaanxi province, China[J]. Plant Disease,2021,105(8):2242.
[61]张丽娜,梅燚,王薇薇,等. 苏北沿海保护地耐盐碱辣椒品系(种)对炭疽病的果期抗性评价[J]. 江苏农业科学,2023,51(13):121-127.
[62]MAHASUK P, KHUMPENG N, WASEE S, et al. Inheritance of resistance to anthracnose (Colletotrichum capsici) at seedling and fruiting stages in chili pepper (Capsicum spp.)[J]. Plant Breeding,2009,128(6):701-706.
[63]SUWOR P, SANITCHON J, THUMMABENJAPONE P, et al. Inheritance analysis of anthracnose resistance and marker-assisted selection in introgression populations of chili (Capsicum annuum L.)[J]. Scientia Horticulturae,2017,220:20-26.
[64]CHEEMA D S, SINGH D P, RAWAL R D, et al. Inheritance of resistance to anthracnose disease in chilli[J]. Vegetable Science,1986,13(2):223-229.
[65]KIM B S, PARK K S, LEE W S. Search for resistance to two Colletotrichum species in pepper (Capsicum spp.)[J]. Journal of Korean Society and Horticultural Science,1987,28:207-213.
[66]MISHRA R, ROUT E, JOSHI R K. Identification of resistant sources against anthracnose disease caused by Colletotrichum truncatum and Colletotrichum gloeosporioides in Capsicum annuum L.[J]. Proceedings of the National Academy of Sciences,2019,89:517-524.
[67]BABA V Y, CONSTANTINO L V, IVAMOTO S T, et al. Capsicum-Colletotrichum interaction:identification of resistance sources and quantification of secondary metabolites in unripe and ripe fruits in response to anthracnose infection[J]. Scientia Horticulturae,2019,246:469-477.
[68]MARACAHIPES C, VISCOVINI K K C G, DA SILVA ANNUNCIATTO E, et al. Genetic diversity of the germplasm active bank of Capsicum of UNEMAT based on components resistant to the fungus Colletotrichum gloeosporioides[J]. Australian Journal of Crop Science,2016,10(7):940-948.
[69]RO N, HAILE M, HUR O, et al. Genome-wide association study of resistance to anthracnose in pepper (Capsicum chinense) germplasm[J]. BMC Plant Biology,2023,23(1):389.
[70]PURIPUNYAVANICH V, NAN T N, SUWAN N, et al. Breeding for anthracnose disease resistance in chili pepper (Capsicum annum L.) using Gamma irradiation[J]. Trends in Sciences,2024,21(8):7709-7709.
[71]PAKDEEVARAPORN P, WASEE S, TAYLOR P W J, et al. Inheritance of resistance to anthracnose caused by Colletotrichum capsici in Capsicum[J]. Plant Breeding,2005,124(2):206-208.
[72]MAHASUK P, TAYLOR P W J, MONGKOLPORN O. Identification of two new genes conferring resistance to Colletotrichum acutatum in Capsicum baccatum[J]. Phytopathology,2009,99(9):1100-1104.
[73]KIM S H, YOON J B, DO J W, et al. A major recessive gene associated with anthracnose resistance to Colletotrichum capsici in chili pepper (Capsicum annuum L.)[J]. Breeding Science,2008,58(2):137-141.
[74]LEE J, HONG J H, DO J W, et al. Identification of QTLs for resistance to anthracnose to two Colletotrichum species in pepper[J]. Journal of Crop Science and Biotechnology,2010,13:227-233.
[75]GARG R, KUMAR S, KUMAR R, et al. Novel source of resistance and differential reactions on chilli fruit infected by Colletotrichum capsici[J]. Australasian Plant Pathology,2013,42:227-233.
[76] GIACOMIN R M, RUAS C F, MOREIRA A F P, et al. Inheritance of anthracnose resistance (Colletotrichum scovillei) in ripe and unripe Capsicum annuum fruits[J]. Journal of Phytopathology,2020,168(3):184-192.
[77]DWIVEDI N, TIRKEY D S, KATOCH S, et al. Evaluation of resistance against anthracnose (Colletotrichum capsici and C. gloeosporioides) in chilli landraces collected from the northeastern region of India[J]. Plant Genetic Resources,2021,19(6):538-544.
[78]RAHMAN M S, AKANDA A M. A major recessive gene associated with anthracnose (Colletotrichum capsici) resistance in chilli pepper[J]. Annals of Agricultural Sciences,2022,7(4):1121-1124.
[79]BAL S, KARAK C, MANDAL A K, et al. Breeding chili pepper for simultaneous improvement in leaf curl virus and anthracnose disease tolerance and commercially important traits[J]. International Journal of Vegetable Science,2024,30(1):91-109.
[80]KIM S H, YOON J B, PARK H G. Inheritance of anthracnose resistance in a new genetic resource,Capsicum baccatum PI594137[J]. Journal of Crop Science and Biotechnology,2008,11(1):13-16.
[81]DE ALMEIDA C L P, SANTOS BENTO C D, SUDR C P, et al. Genotype-Ideotype distance index and multivariate analysis to select sources of anthracnose resistance in Capsicum spp.[J]. European Journal of Plant Pathology,2020,156(1):223-236.
[82]LY V A, TRUONG T P T, NGUYEN T H. Application of anthracnose resistance-associated molecular markers in the detection of resistant chili pepper cultivars in Vietnam[J]. Science and Technology Development Journal,2020,23(3):581-589.
[83]RO N Y, SEBASTIN R, HUR O S, et al. Evaluation of anthracnose resistance in pepper (Capsicum spp.) genetic resources[J]. Horticulturae,2021,7(11):460-475.
[84]刘建华,杨玉珍,邹学校,等. 辣(甜)椒种质资源苗期对炭疽病的抗性鉴定[J]. 陕西农业科学,1991(4):11-13.
[85]黄任中,吕中华,林清,等. 加工型辣椒抗炭疽病材料筛选初报[J]. 辣椒杂志,2005(1):17-19.
[86]吴庆丽,秦刚. 辣椒炭疽病抗性资源筛选[J]. 湖北农业科学,2013,52(5):1084-1089.
[87]张国芝,赵霞,杨海艳,等. 四川辣椒炭疽病菌鉴定及育种材料抗性筛选[J]. 西南农业学报,2013,26(3):1026-1029.
[88]张世才,李怡斐,王春萍,等. 加工型辣椒抗炭疽病材料的筛选[C]//中国园艺学会.中国园艺学会2018年学术年会论文摘要集. 青岛:中国园艺学会,2018.
[89]彭泽,胡明文,廖芳芳,等. 辣椒抗炭疽病品种(材料)筛选及鉴定[J]. 辣椒杂志,2022,20(2):1-5.
[90]于海龙,靳远,周黛媛,等. 内蒙古地区辣椒种质资源抗病性鉴定与评价[J]. 中国蔬菜,2023(11):69-79.
[91]WANG Y X, CHEN B, CHENG C Y, et al. Comparative transcriptomics analysis reveals the differences in transcription between resistant and susceptible pepper (Capsicum annuum L.) varieties in response to anthracnose[J]. Plants,2024,13(4):527.
[92]戴丽,谢芳玲,陈雅倩,等. 辣椒抗炭疽病材料筛选及转录组分析[J]. 湖南农业大学学报(自然科学版),2025,51(1):40-50.
[93]张红肖,孟雅宁,张哲,等. 辣椒资源抗性基因分子标记检测[J]. 河北农业科学,2024,28(6):70-74.
[94] LEE J, DO J W, YOON J B. Development of STS markers linked to the major QTLs for resistance to the pepper anthracnose caused by Colletotrichum acutatum and C. capsici[J]. Horticulture,Environment,and Biotechnology,2011,52:596-601.
[95]SUN C Y, MAO S L, ZHANG Z H, et al. Resistances to anthracnose (Colletotrichum acutatum) of Capsicum mature green and ripe fruit are controlled by a major dominant cluster of QTLs on chromosome P5[J]. Scientia Horticulturae,2015,181:81-88.
[96]MAHASUK P, STRUSS D, MONGKOLPORN O. QTLs for resistance to anthracnose identified in two Capsicum sources[J]. Molecular Breeding,2016,36:1-10.
[97]刘议蔚. 辣椒抗炭疽病主效基因AnRGO5精细定位[D]. 北京:中国农业科学院,2017.
[98]MISHRA R, ROUT E, MOHANTY J N, et al. Sequence-tagged site-based diagnostic markers linked to a novel anthracnose resistance gene RCt1 in chili pepper (Capsicum annuum L.)[J]. 3 Biotech,2019,9:9-21.
[99]ZHAO Y Y, LIU Y W, ZHANG Z H, et al. Fine mapping of the major anthracnose resistance QTL AnRGO5 in Capsicum chinense ‘PBC932’[J]. BMC Plant Biology,2020,20:189-196.
[100]KETHOM W, MONGKOLPORN O. New QTLs for anthracnose resistance identified in Capsicum baccatum ‘PBC80’-derived recombinant inbred lines[J]. Euphytica,2021,217:128-139.
[101]JO J, KIM G W, BACK S, et al. Exploring horticultural traits and disease resistance in Capsicum baccatum through segmental introgression lines[J]. Theoretical and Applied Genetics,2023,136(11):233.
[102]周黛媛. 辣椒果实成熟期抗炭疽病主效QTL AnT5.1的定位[D]. 北京:中国农业科学院,2023.
[103]DA COSTA GERONIMO I G, BIANCHI P A, DE ARAUJO M S B, et al. Resistance to anthracnose (Colletotrichum scovillei) in Capsicum annuum:inheritance,QTL identification and progenies selection to develop resistant cultivars[J]. Functional Plant Breeding Journal,2024,6:1-20.
[104]李怡斐,段敏杰,黄任中,等. 基于SLAF-Seq技术的辣椒抗炭疽病QTL定位分析及SSR分子标记开发[J]. 南方农业学报,2025,56(2):633-642.
[105]OH B J, KO M K, KIM Y S, et al. A cytochrome P450 gene is differentially expressed in compatible and incompatible interactions between pepper (Capsicum annuum) and the anthracnose fungus,Colletotrichum gloeosporioides[J]. Molecular Plant-Microbe Interactions,1999,12(12):1044-1052.
[106]KIM Y S, LEE H H, KO M K, et al. Inhibition of fungal appressorium formation by pepper (Capsicum annuum) esterase[J]. Molecular Plant-Microbe Interactions,2001,14(1):80-85.
[107]SHARMA R, MAHANTY B, MISHRA R, et al. Genome wide identification and expression analysis of pepper C2H2 zinc finger transcription factors in response to anthracnose pathogen Colletotrichum truncatum[J]. 3 Biotech,2021,11(3):118-135.
[108]MISHRA R, MOHANTY J N, MAHANTY B, et al. A single transcript CRISPR/Cas9 mediated mutagenesis of CaERF28 confers anthracnose resistance in chilli pepper (Capsicum annuum L.)[J]. Planta,2021,254(1):5-21.
[109]LEE H A, KIM S, CHOI D, et al. Expansion of sesquiterpene biosynthetic gene clusters in pepper confers nonhost resistance to the Irish potato famine pathogen[J]. New Phytologist,2017,215(3):1132-1143.
[110]PARK S, PARK A R, IM S, et al. Developmentally regulated sesquiterpene production confers resistance to Colletotrichum gloeosporioides in ripe pepper fruits[J]. PLoS One,2014,9(10):e109453.
[111]PADILHA H K M, MADRUGA N A, ARANHA B C, et al. Defense responses of Capsicum spp. genotypes to post-harvest Colletotrichum sp. inoculation[J]. Phytoparasitica,2019,47:557-573.
[112]EDIRISINGHE M, ALI A, MAQBOOL M, et al. Chitosan controls postharvest anthracnose in bell pepper by activating defense-related enzymes[J]. Journal of Food Science and Technology,2014,51:4078-4083.
[113]ABHAYASHREE M S, MURALI M, AMRUTHESH K N. Abiotic elicitors mediated resistance and enhanced defense related enzymes in Capsicum annuum L. against anthracnose disease[J]. Scientia Horticulturae,2016,204:172-178.
[114]PARK S, JEONG W Y, LEE J H, et al. Determination of polyphenol levels variation in Capsicum annuum L. cv. Chelsea (yellow bell pepper) infected by anthracnose (Colletotrichum gloeosporioides) using liquid chromatography-tandem mass spectrometry[J]. Food Chemistry,2012,130(4):981-985.
[115]EFFANTIN G, RIVASSEAU C, GROMOVA M, et al. Massive production of butanediol during plant infection by phytopathogenic bacteria of the genera Dickeya and Pectobacterium[J]. Molecular Microbiology,2011,82(4):988-997.
[116]JAYAPALA N, MALLIKARJUNAIAH N H, PUTTASWAMY H, et al. Rhizobacteria Bacillus spp. induce resistance against anthracnose disease in chili (Capsicum annuum L.) through activating host defense response[J]. Egyptian Journal of Biological Pest Control,2019,29(1):1-9.