[1]李洁,宋子微,韩倩,等.耐旱促生复合菌群的构建及其对干旱胁迫下作物的促生作用[J].江苏农业学报,2026,42(02):314-324.[doi:doi:10.3969/j.issn.1000-4440.2026.02.010]
　LI Jie,SONG Ziwei,HAN Qian,et al.Construction of drought-tolerant and growth-promoting microbial communities and their growth-promoting effects on crops under drought stress[J].,2026,42(02):314-324.[doi:doi:10.3969/j.issn.1000-4440.2026.02.010]
点击复制

耐旱促生复合菌群的构建及其对干旱胁迫下作物的促生作用()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年02期
页码:
314-324
栏目:
耕作栽培·资源环境
出版日期:
2026-02-28

文章信息/Info

Title:
Construction of drought-tolerant and growth-promoting microbial communities and their growth-promoting effects on crops under drought stress
作者:
李洁; 宋子微; 韩倩; 于小喧; 孟庆欣; 莫继先
(齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006)
Author(s):
LI Jie; SONG Ziwei; HAN Qian; YU Xiaoxuan; MENG Qingxin; MO Jixian
(College of Life Sciences and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China)
关键词:
耐旱促生复合菌群; 干旱胁迫; 绿豆; 小麦; 促生作用
Keywords:
drought-tolerant and plant-growth-promoting synthetic microbial community; drought stress; mung bean; wheat; growth-promoting effect
分类号:
S154.3
DOI:
doi:10.3969/j.issn.1000-4440.2026.02.010
文献标志码:
A
摘要:
为了应对干旱胁迫对农作物的威胁,本研究从黑龙江省齐齐哈尔市梅里斯达斡尔族区受风蚀影响出现土壤沙化现象的农田土壤中分离并筛选多株耐旱促生菌株。耐旱能力分析结果显示,筛选出的菌株中N1、N6、N8、K5、J7表现较好；促生能力分析结果显示,筛选出的菌株具有溶磷、解钾、产铁载体和分泌吲哚乙酸（IAA）等多种植物促生特性。通过拮抗试验和玉米促生试验确定了普里斯特菌属细菌N6菌株（Priestia sp.N6）、芽孢杆菌属细菌N8菌株（Bacillus sp.N8）、假单胞菌属细菌K5菌株（Pseudomonas sp.K5）和不动杆菌属细菌J7菌株（Acinetobacter sp.J7）为优选菌株,用于构建复合菌群DSM。施用复合菌群DSM对干旱胁迫下绿豆和小麦的生长产生了积极作用,与CK相比,两种作物株高、苗鲜重、苗干重、根长、根鲜重及叶绿素含量都显著提升,根冠比分别下降了34.04%和40.63%。此外,复合菌群DSM还显著提高了绿豆和小麦叶片中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等抗氧化酶活性,降低了土壤pH,提高了土壤速效磷和速效钾含量。本研究构建的复合菌群为干旱地区农业可持续发展提供了新方案。
Abstract:
In response to the threat of drought stress to crops, this study isolated and screened multiple drought-tolerant and plant-growth-promoting bacterial strains from the farmland soils in the Meilisi Daur District of Qiqihar City, Heilongjiang province, where wind erosion has led to soil desertification. The results of the drought tolerance analysis showed that strains N1, N6, N8, K5, and J7 performed well. Analysis of plant-growth-promoting traits revealed that the selected strains exhibited multiple beneficial properties, such as phosphate solubilization, potassium mobilization, siderophore production, and secretion of indole-3-acetic acid (IAA). Through antagonism assays and maize growth promotion experiments, Priesti sp. N6, Bacillus sp. N8, Pseudomonas sp. K5, and Acinetobacter sp. J7 were identified as the preferred strains for the construction of the synthetic microbial community DSM. The application of DSM exerted positive effects on the growth of mung beans and wheat under drought stress. Compared with the control (CK), both crops showed significant increases in plant height, seedling fresh weight, seedling dry weight, root length, root fresh weight and chlorophyll content, while the root-to-shoot ratio decreased by 34.04% and 40.63%, respectively. Additionally, the application of DSM significantly enhanced the activities of antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), in the leaves of mung beans and wheat. It also reduced soil pH and increased the content of available phosphorus and available potassium in the soil. The synthetic microbial community constructed in this study provides a new strategy for sustainable agricultural development in arid regions.

参考文献/References:

[1]路捷,郭秀峰. 全球变暖对中国东部沿海地区农业的影响研究[J]. 山西农经,2022(13):1-4.
[2]葛道阔,曹宏鑫,马晓群,等. 基于作物生长模型的小麦旱涝敏感性分析与损失评估[J]. 江苏农业学报,2016,32(6):1302-1309.
[3]王世豪,徐新良,曹巍. 2000-2020年东北黑土地土壤侵蚀时空演化特征[J]. 资源科学,2023,45(5):951-965.
[4]邓蓓,王晓锋,廖君. 环境胁迫影响三峡库区消落带草本和木本植物生理生态特征的meta分析 [J]. 植物生态学报,2024,48(5):623-637.
[5]DERIBE H. Review on effects of drought stress on maize growth,yield and its management strategies[J]. Communications in Soil Science and Plant Analysis,2025,56(1):123-143.
[6]WAN Y X, KAPOOR R, DA SILVA F S B, et al. Elucidating the mechanism regarding enhanced tolerance in plants to abiotic stress by Serendipita indica[J]. Plant Growth Regulation,2024,103(2):271-281.
[7]WANG Y, CAO J L, HASHEM A, et al. Serendipita indica mitigates drought-triggered oxidative burst in trifoliate orange by stimulating antioxidant defense systems[J]. Frontiers in Plant Science,2023,14:1247342.
[8]OKPARA J N, OGUNJOBI K O, ADEFISAN E A. Developing objective dry spell and drought triggers for drought monitoring in the Niger Basin of West Africa[J]. Natural Hazards,2022,112(3):2465-2492.
[9]周士锋, 秦仁强. 盐胁迫下植物促生菌和2,4-表油菜素内酯对月季幼苗光合生理及离子转运的影响[J]. 河南农业科学,2024,53(10):138-148.
[10]杨凡. 放线菌菌剂对旱区煤矿排土场碱茅、红豆草生长及根际土壤性质的调控[D]. 西安:西安建筑科技大学,2023.
[11]CHAI Q, GAN Y T, ZHAO C, et al. Regulated deficit irrigation for crop production under drought stress. A review[J]. Agronomy for Sustainable Development,2015,36(1):3.
[12]李雪梅,姚拓,杨晓蕾,等. 干旱地区植物根际促生菌鉴定及促生和耐旱特性研究[J]. 中国草地学报,2024,46(9):87-95.
[13]陈秦,薛泉宏,申光辉,等. 放线菌对棉花幼苗生长及抗旱能力的影响[J]. 西北农业学报,2010,19(8):84-89.
[14]李安,舒健虹,刘晓霞,等. 干旱胁迫下枯草芽孢杆菌对玉米种子抗旱性及生理指标的影响[J]. 作物杂志,2021(6):217-223.
[15]舒健虹,王子苑,曾庆飞,等. 基于转录组测序分析阿氏芽孢杆菌R60对玉米抗旱性的影响[J]. 南方农业学报,2023,54(8):2340-2351.
[16]冯海萍,陈卓,杨虎. 微生物菌剂对连作芹菜根际土壤真菌群落多样性与结构的影响[J]. 干旱地区农业研究,2024,42(2):53-61,70.
[17]邹宜芯,李慧,任玉欣,等. 硫代葡萄糖苷浸种对青花菜种子萌发及生理特性的影响[J]. 江苏农业学报,2024,40(8):1379-1388.
[18]杨艳华. 土壤解磷微生物的分离鉴定[D]. 郑州:河南农业大学,2014.
[19]鞠伟. 杨树根际高效解钾细菌的分离筛选与鉴定[D]. 南京:南京林业大学,2016.
[20]汤子祥. 艾草根系促生菌的筛选鉴定及其应用效果研究[D]. 杨凌:西北农林科技大学,2022.
[21]王娟娟. 肥效微生物筛选及对小麦促生效果的研究[D]. 杨凌:西北农林科技大学,2019.
[22]贾丹莉,杨治平,郭军玲,等. 6种玉米品种耐盐性筛选[J]. 中国农学通报,2017,33(11):1-8.
[23]马献发. 土壤学实验[M]. 北京:中国林业出版社,2020.
[24]王晓雨,王小平,史文宇,等. 拔节期冬小麦光合特性、干物质积累和产量对干旱胁迫的响应[J]. 新疆农业科学,2023,60(9):2163-2172.
[25]SHAHID M, ZEYAD M T, SYED A, et al. Stress-tolerant endophytic isolate Priestia aryabhattai BPR-9 modulates physio-biochemical mechanisms in wheat (Triticum aestivum L.) for enhanced salt tolerance [J]. International Journal of Environmental Research and Public Health,2022,19(17):10883.
[26]ABIALA M, SADHUKHAN A, MUTHUVEL J, et al. Rhizosphere Priestia species altered cowpea root transcriptome and enhanced growth under drought and nutrient deficiency[J]. Planta,2022,257(1):11.
[27]PETER O, IMRAN M, SHAFFIQUE S, et al. Combined application of melatonin and Bacillus sp. strain IPR-4 ameliorates drought stress tolerance via hormonal,antioxidant,and physiomolecular signaling in soybean[J]. Frontiers in Plant Science,2024,15:1274964.
[28]KHAN A, SINGH A V. Multifarious effect of ACC deaminase and EPS producing Pseudomonas sp. and Serratia marcescens to augment drought stress tolerance and nutrient status of wheat[J]. World Journal of Microbiology & Biotechnology,2021,37(12):198.
[29]ANSARI F A, AHMAD I. Isolation,functional characterization and efficacy of biofilm-forming rhizobacteria under abiotic stress conditions[J]. Antonie Van Leeuwenhoek,2019,112(12):1827-1839.
[30]李妍,胡斯乐,白晓雄,等. 核桃根际耐旱促生菌的分离筛选及其促生作用研究[J]. 西北林学院学报,2024,39(3):1-9.
[31]王一腾,钱怡雯,刁琦,等. 耐旱菌研究热点变迁及其提高植物耐旱性的机制[J]. 微生物学通报,2024,51(11):4370-4382.
[32]李冬. 毛果杨耐旱细菌菌群的构建及其耐旱促生的生理机制初探[D]. 哈尔滨:东北林业大学,2023.
[33]陈颖慧,佘冰雨,葛才林,等. 作物耐低磷机制研究进展[J]. 安徽农业科学,2024,52(8):1-5.
[34]CHEN X X, ZHANG W, LIANG X Y, et al. Physiological and developmental traits associated with the grain yield of winter wheat as affected by phosphorus fertilizer management[J]. Scientific Reports,2019,9:16580.
[35]闫金垚. 磷肥用量对水稻-油菜轮作系统作物产量与磷肥利用及土壤磷素转化的影响[D]. 武汉:华中农业大学,2022.
[36]梁俊捷. 气候、地形和土壤性质对我国农田土壤有效钾分布的影响[D]. 雅安:四川农业大学,2018.
[37]马悦,田怡,牟文燕,等. 北方麦区小麦产量与籽粒氮磷钾含量对监控施钾和土壤速效钾的响应[J]. 中国农业科学,2022,55(16):3155-3169.

相似文献/References:

[1]佚名 佚名 佚名.三才期刊采编系统文章正在整理中…[J].江苏农业学报,2005,(01):5.
　XIE Xin,ZHAO Zhong.三才期刊采编系统文章正在整理中…[J].,2005,(02):5.
[2]佚名 佚名 佚名.三才期刊采编系统文章正在整理中…[J].江苏农业学报,2006,(01):5.
　XIE Xin,ZHAO Zhong.三才期刊采编系统文章正在整理中…[J].,2006,(02):5.
[3]熊洁,邹晓芬,邹小云,等.干旱胁迫对不同基因型油菜农艺性状和产量的影响[J].江苏农业学报,2015,(03):494.[doi:10.3969/j.issn.1000-4440.2015.03.005]
　XIONG Jie,ZOU Xiao-fen,ZOU Xiao-yun,et al.Effects of drought stress on agronomic traits and yield of different rapeseed genotypes[J].,2015,(02):494.[doi:10.3969/j.issn.1000-4440.2015.03.005]
[4]肇莹,杨镇,杨涛,等.植物内生菌醇提取物对草坪草抗旱性的影响[J].江苏农业学报,2015,(01):39.[doi:10.3969/j.issn.1000-4440.2015.01.006]
　ZHAO Ying,YANG Zhen,YANG Tao,et al.Influence of plant endophyte extract on turfgrass drought tolerance[J].,2015,(02):39.[doi:10.3969/j.issn.1000-4440.2015.01.006]
[5]孟力力,张俊,闻婧.干旱胁迫对彩叶草光合特性及叶片超微结构的影响[J].江苏农业学报,2015,(01):180.[doi:10.3969/j.issn.1000-4440.2015.01.028]
　MENG Li-li,ZHANG Jun,WEN Jing.Changes of photosynthetic characteristics of Coleus blumei and mesophyll cell ultrastructure in response to drought stress[J].,2015,(02):180.[doi:10.3969/j.issn.1000-4440.2015.01.028]
[6]张丽丽,徐碧玉,刘菊华,等.MaASR1基因通过乙烯途径提高拟南芥抗旱性的作用机制[J].江苏农业学报,2018,(03):511.[doi:doi:10.3969/j.issn.1000-4440.2018.03.005]
　ZHANG Li-li,XU Bi-yu,LIU Ju-hua,et al.The regulation mechanism of MaASR1 gene for improving the drought resistance of Arabidopsis by ethylene pathway[J].,2018,(02):511.[doi:doi:10.3969/j.issn.1000-4440.2018.03.005]
[7]麻云霞,李钢铁,张宏武,等.外源硅对酸枣生长和生理生化特征的影响[J].江苏农业学报,2018,(05):1113.[doi:doi:10.3969/j.issn.1000-4440.2018.05.021]
　MA Yun-xia,LI Gang-tie,ZHANG Hong-wu,et al.Effects of exogenous silicon on growth， physiological and biochemical characteristics of zizyphus jujube plant[J].,2018,(02):1113.[doi:doi:10.3969/j.issn.1000-4440.2018.05.021]
[8]葛道阔,曹宏鑫,杨余旺,等.干旱胁迫下油菜栽培模拟优化决策系统（Rape-CSODS）的订正及其检验[J].江苏农业学报,2019,(01):56.[doi:doi:10.3969/j.issn.1000-4440.2019.01.008]
　GE Dao-kuo,CAO Hong-xin,YANG Yu-wang,et al.Modification and verification of Rape-CSODS under drought stress[J].,2019,(02):56.[doi:doi:10.3969/j.issn.1000-4440.2019.01.008]
[9]陈丽,焦健,朱绍丹,等.油橄榄对牧草间作与干旱胁迫交互作用的根系生理响应[J].江苏农业学报,2019,(06):1434.[doi:doi:10.3969/j.issn.1000-4440.2019.06.024]
　CHEN Li,JIAO Jian,ZHU Shao-dan,et al.Root physiological response of olive to the interaction of pasture intercropping and drought stress[J].,2019,(02):1434.[doi:doi:10.3969/j.issn.1000-4440.2019.06.024]
[10]陈丽,焦健,朱绍丹,等.牧草间作对干旱胁迫下油橄榄根系形态特征的影响[J].江苏农业学报,2020,(01):39.[doi:doi:10.3969/j.issn.1000-4440.2020.01.006]
　CHEN Li,JIAO Jian,ZHU Shao-dan,et al.Effects of pasture intercropping on root morphological characteristics of olive under drought stress[J].,2020,(02):39.[doi:doi:10.3969/j.issn.1000-4440.2020.01.006]

备注/Memo

备注/Memo:
收稿日期：2024-12-05基金项目：黑龙江省省属高等学校基本科研业务费项目(145409453)作者简介：李洁(1998-),女,山东济宁人,硕士研究生,研究方向是资源环境微生物学。(E-mail)joyceleepp@163.com通讯作者：莫继先,(E-mail)mojixian8208@sina.com
更新日期/Last Update: 2026-03-16