[1]杨晓菲,张宁,张树军.外源精胺和亚精胺对盐胁迫下2种小麦幼苗生理特性的影响[J].江苏农业学报,2026,42(01):21-31.[doi:doi:10.3969/j.issn.1000-4440.2026.01.003]
　YANG Xiaofei,ZHANG Ning,ZHANG Shujun.Effects of exogenous spermine and spermidine on the physiological characteristics of two wheat seedlings under salt stress[J].,2026,42(01):21-31.[doi:doi:10.3969/j.issn.1000-4440.2026.01.003]
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外源精胺和亚精胺对盐胁迫下2种小麦幼苗生理特性的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年01期
页码:
21-31
栏目:
遗传育种·生理生化
出版日期:
2026-01-31

文章信息/Info

Title:
Effects of exogenous spermine and spermidine on the physiological characteristics of two wheat seedlings under salt stress
作者:
杨晓菲1; 2; 张宁1; 张树军3
(1.渭南师范学院环境与生命科学学院,陕西渭南714000；2.陕西省河流湿地生态与环境重点实验室,陕西渭南714000；3.陕西省华阴市种子管理站,陕西华阴714200)
Author(s):
YANG Xiaofei1; 2; ZHANG Ning1; ZHANG Shujun3
(1.College of Environment and Life Sciences, Weinan Normal University, Weinan 714000, China;2.Key Laboratory for Ecology and Environment of River Wetlands in Shaanxi Province, Weinan 714000, China；3.Huayin Seed Management Station, Huayin 714200, China)
关键词:
小麦; 盐胁迫; 外源精胺; 外源亚精胺; 生长指标; 生理特性
Keywords:
wheat; salt stress; exogenous spermine; exogenous spermidine; growth parameters; physiological characteristics
分类号:
S512
DOI:
doi:10.3969/j.issn.1000-4440.2026.01.003
文献标志码:
A
摘要:
为了探讨外源精胺(Spm)和亚精胺(Spd)对小麦幼苗生长和耐盐性的影响,本试验选用耐盐性较强的百农201和耐盐性较弱的矮抗58 2个小麦品种为材料,探究3种浓度(0.05 mmol/L、0.10 mmol/L、0.20 mmol/L)的Spm和Spd浸种对不同程度盐胁迫(25 mmol/L NaCl、50 mmol/L NaCl、100 mmol/L NaCl)下小麦生长指标(发芽率、根长和芽长)和生理指标[叶绿素(Chl.)含量、丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化物酶(POD)活性]的调节效应。结果表明,与对照（纯水培养）相比,NaCl胁迫显著抑制了小麦种子萌发和幼苗生长,使种子发芽率、根长、芽长、Chl.含量显著下降,MDA含量、Pro含量和POD活性升高；适宜浓度Spm和Spd浸种可缓解盐胁迫对百农201和矮抗58种子发芽率、根长、芽长及叶绿素含量的抑制作用,使MDA含量下降，Pro含量和POD活性进一步提高。结合隶属函数法综合分析结果表明,Spd较Spm能更好地减轻盐胁迫对幼苗的毒害作用,且对盐敏感品种矮抗58的作用效果好于耐盐品种百农201,其中0.10 mmol/L Spd浸种缓解NaCl胁迫对小麦幼苗毒害的效果较好。
Abstract:
To investigate the effects of exogenous spermine (Spm) and spermidine (Spd) on the growth and salt tolerance of wheat seedlings, this experiment used two wheat varieties with different salt tolerances (Bainong 201, with strong salt tolerance; Aikang 58, with weak salt tolerance) as materials. It explored the regulatory effects of seed soaking with three concentrations (0.05 mmol/L, 0.10 mmol/L, and 0.20 mmol/L) of Spm and Spd on the growth parameters (germination rate, root length and shoot length) and physiological indicators including chlorophyll (Chl.) content, malondialdehyde (MDA) content, proline (Pro) content and peroxidase (POD) activity of wheat seedlings under different degrees (25 mmol/L NaCl, 50 mmol/L NaCl, and 100 mmol/L) of NaCl stress. The results showed that, compared with the control (cultivation in distilled water), NaCl stress significantly inhibited wheat seed germination and seedling growth. The germination rate, root length, shoot length, chlorophyll content of Bainong 201 and Aikang 58 decreased significantly as the salt stress concentration increased, while the MDA content, Pro content, and POD activity increased significantly. Seed soaking with appropriate concentrations of Spm and Spd alleviated the inhibitory effects of salt stress on seed germination rate, root length, shoot length, and chlorophyll content in both Bainong 201 and Aikang 58. This treatment resulted in a decrease in MDA content and a further increase in Pro content and POD activity. Based on a comprehensive analysis using the membership function method, the results indicated that Spd was more effective than Spm in alleviating the toxic effects of salt stress on seedlings. Furthermore, its positive effect was more pronounced in the salt-sensitive variety Aikang 58 than in the salt-tolerant variety Bainong 201. Among the treatments, seed soaking with 0.10 mmol/L Spd demonstrated the best efficacy in mitigating NaCl stress-induced damage to wheat seedlings.

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备注/Memo

备注/Memo:
收稿日期：2025-03-03基金项目：陕西省科技厅重点研发计划——农业农村领域项目(2025NC-YBXM-004)；国家自然科学基金青年项目(31701416)；陕西省科协青年人才托举计划项目(20200207)；国家级大学生创新创业训练计划项目(202310723026)作者简介：杨晓菲(1988-),女,陕西渭南人,博士,副教授,主要从事作物生理研究。(E-mail)yangxiaofei2007@yeah.net通讯作者：张树军,(E-mail)865728077@qq.com
更新日期/Last Update: 2026-02-09