[1]刘发旺,张银,李璐恒,等.基于代谢组与转录组联合解析干旱胁迫下丹参酮和丹酚酸生物合成的关键调控基因及其分子机制[J].江苏农业学报,2025,(11):2107-2118.[doi:doi:10.3969/j.issn.1000-4440.2025.11.004]
　LIU Fawang,ZHANG Yin,LI Luheng,et al.Analysis of the key regulatory genes and molecular mechanisms of tanshinone and salvianolic acid biosynthesis under drought stress through integrated metabolomics and transcriptomics[J].,2025,(11):2107-2118.[doi:doi:10.3969/j.issn.1000-4440.2025.11.004]
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基于代谢组与转录组联合解析干旱胁迫下丹参酮和丹酚酸生物合成的关键调控基因及其分子机制()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年11期
页码:
2107-2118
栏目:
遗传育种·生理生化
出版日期:
2025-11-30

文章信息/Info

Title:
Analysis of the key regulatory genes and molecular mechanisms of tanshinone and salvianolic acid biosynthesis under drought stress through integrated metabolomics and transcriptomics
作者:
刘发旺1; 2; 张银1; 李璐恒1; 吕欣茹1; 许盈盈1
(1.宿州学院生物与食品工程学院,安徽宿州234000；2.安徽省皖北道地药材开发与高值化利用工程研究中心,安徽宿州234000)
Author(s):
LIU Fawang1; 2; ZHANG Yin1; LI Luheng1; LYU Xinru1; XU Yingying1
(1.School of Biology and Food Engineering, Suzhou University, Suzhou 234000, China；2.Engineering Research Center for Development and High Value Utilization of Genuine Medicinal Materials in North Anhui Province, Suzhou 234000, China)
关键词:
干旱胁迫; 丹参; 转录组; 代谢组; 丹参酮
Keywords:
drought stress; Salvia miltiorrhiza Bge.; transcriptome; metabolome; tanshinone
分类号:
S567.23
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.004
文献标志码:
A
摘要:
本研究采用20%聚乙二醇6000（PEG 6000）处理丹参,结合代谢组和转录组,系统解析干旱胁迫下丹参酮和丹酚酸生物合成的调控机制。研究结果表明,与对照相比,PEG 6000处理丹参中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性极显著降低(P<0001),苯丙氨酸解氨酶(PAL)活性则极显著升高(P<0001)。代谢组分析结果表明,与对照相比,PEG 6000处理丹参中丹参酮类化合物如丹参酮ⅡA、丹参酮Ⅰ、二氢丹参酮和隐丹参酮含量显著(P<005)或极显著升高(P<001),表明干旱胁迫能有效促进二萜类化合物的积累。与对照相比,PEG 6000处理丹参中丹酚酸类化合物丹酚酸B含量极显著降低(P<0001),表明其生物合成途径受到干旱胁迫的抑制。与对照相比,PEG 6000处理丹参中含量升高的代谢产物和差异表达基因显著富集在二萜生物合成通路中。通过转录组测序筛选到3个参与丹参酮合成的基因,分别为CYP76AH4、CYP71D411、CYP82D63,这3个基因均注释到二萜生物合成通路中。与对照相比,PEG 6000处理丹参根中EVM0001349、EVM0020320相对表达量极显著升高(P<001),EVM0023606相对表达量显著降低(P<005)。对照丹参叶片中EVM0001349、EVM0020320和EVM0023606相对表达量极显著高于根系(P<0001)。生物信息学分析结果表明,EVM0001349、EVM0020320和EVM0023606这3个细胞色素P450基因可能参与丹参酮的结构修饰。本研究结果为提高丹参药用品质提供了理论依据。
Abstract:
In this study, Salvia miltiorrhiza Bge. was treated with 20% polyethylene glycol 6000 (PEG 6000), and a combination of metabolomics and transcriptomics was used to systematically elucidate the re-gulatory mechanisms of tanshinone and salvianolic acid biosynthesis under drought stress. The results showed that compared with the control, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in PEG 6000-treated Salvia miltiorrhiza Bge. reduced extremely significantly (P<0001), while the activity of phenylalanine ammonia-lyase (PAL) increased extremely significantly (P<0001). Metabolomic analysis revealed that compared with the control, the contents of tanshinone compounds such as tanshinone ⅡA, tanshinone Ⅰ, dihydrotanshinone, and cryptotanshinone in PEG6000-treated Salvia miltiorrhiza Bge. increased significantly (P<005) or extremely significantly (P<001), indicating that drought stress could effectively promote the accumulation of diterpenoid compounds. Compared with the control, the content of compound salvianolic acid B in PEG 6000-treated Salvia miltiorrhiza Bge. reduced extremely significantly (P<0001), indicating that its biosynthetic pathway was inhibited by drought stress. Compared with the control, the metabolites with increased content and differentially expressed genes in PEG 6000-treated Salvia miltiorrhiza Bge. were significantly enriched in the diterpenoid biosynthesis pathway. Three genes involved in tanshinone synthesis were screened through transcriptome sequencing, namely CYP76AH4, CYP71D411, and CYP82D63, all of which were annotated in the diterpenoid biosynthesis pathway. Compared with the control, the relative expression levels of EVM0001349 and EVM0020320 in the roots of PEG 6000-treated Salvia miltiorrhiza Bge. increased extremely significantly (P<001), while the relative expression level of EVM0023606 reduced significantly(P<005). Moreover, the relative expression levels of EVM0001349, EVM0020320, and EVM0023606 in the leaves of control Salvia miltiorrhiza Bge. were extremely significantly higher than those in the roots (P<0001). Bioinformatics analysis indicated that these three cytochrome P450 genes might be involved in the structural modification of tanshinones. This study provides a theoretical basis for improving the medicinal quality of Salvia miltiorrhiza Bge..

参考文献/References:

[1]杨彬,赵文博,张海燕,等. 丹参资源的遗传多样性及其保护利用[J]. 寒旱农业科学,2023,2(11):1002-1008.
[2]史顺敏,俞年军,于凡,等. 丹参种质资源的研究进展[J]. 现代中医药,2016,36(6):113-117.
[3]陈雨萌,李倩,刘维海,等. 丹参活性成分治疗心血管疾病的药理作用、临床应用及不良反应研究进展[J]. 药学研究,2023,42(12):1028-1034.
[4]黄丽丽,苏静. 丹参的作用机制及临床应用研究进展[J]. 中国药物滥用防治杂志,2003,29(6):1002-1006.
[5]徐怡,陈途,陈明. 丹参的化学成分及其药理作用研究进展[J]. 海峡药学,2021,33(5):45-48.
[6]刘会会,尹佳,强明敏,等. 丹参多酚酸盐药理作用研究进展[J]. 中国当代医药,2021,28(22):32-36.
[7]国家药典委员会. 中华人民共和国药典:一部[M]. 北京:中国医药科技出版社,2020.
[8]SHOU M Y, LIN Q Z, XU Y, et al. New insights of advanced biotechnological engineering strategies for tanshinone biosynthesis in Salvia miltiorrhiza[J]. Plant Science，2025,352:112384.
[9]詹忠根,李杏. 丹参酮和丹酚酸类化合物的生物合成及其转录调控机制[J]. 药学学报,2020,55(9):2110-2121.
[10]曾慧婷,宿树兰,朱悦,等. 丹参酚酸类成分生物合成途径及调控机制研究进展[J]. 中草药,2016,47(18):3324-3331.
[11]GUPTA A,RICO M,CANO D. The physiology of plant responses to drought[J]. Science,2020,368(6488):266-269.
[12]李娟,陈杰忠,胡又离,等. 水分胁迫对柑橘果皮细胞壁结构与代谢的影响[J]. 生态学报,2008,28(2):486-492.
[13]董馥慧,裴红宾,张永清,等. 干旱胁迫与复水对苦荞生长及叶片内源激素含量的影响[J]. 中国农业科技导报,2019,21(12):41-48.
[14]崔文婷,孟玉山,张昱,等. 药用植物干旱胁迫响应研究进展[J]. 南方农业,2019,13(增刊1):151-156.
[15]BISTGANI Z E, BARKER A V, HASHEMI M, et al. Physiology of medicinal and aromatic plants under drought stress[J]. The Crop Journal,2024,12(2):330-339.
[16]白禹,卯丹,王继玥. 黄酮类物质的生物合成及对干旱胁迫响应的研究进展[J]. 安徽农业科学,2018,46(16):24-26.
[17]谭勇,梁宗锁,董娟娥,等. 水分胁迫对菘蓝生长发育和有效成分积累的影响[J]. 中国中药杂志,2008,33(1):19-22.
[18]李霞,王洋,阎秀峰. 水分胁迫对黄檗幼苗三种生物碱含量的影响[J]. 生态学报,2007,27(1):58-64.
[19]周丽,王永明,周达,等. 干旱胁迫对银柴胡药材活性成分含量的影响研究[J]. 时珍国医国药,2015,26(6):1463-1465.
[20]易家宁,王康才,张琪绮,等. 干旱胁迫对紫苏生长及品质的影响[J]. 核农学报,2020,34(6):1320-1326.
[21]YANG Y,XU Y,FENG B,et al. Regulatory networks of bZIPs in drought,salt and cold stress response and signaling[J]. Plant Science，2025,352:112399.
[22]谢荟清,苏文娟,赵娜红,等. 油茶bHLH基因家族鉴定与响应干旱胁迫的基因表达分析[J].中南林业科技大学学报,2025,45(1):168-180.
[23]LI W R, YU J, WANG R H, et al. Overexpression of SmGRAS5 enhances tolerance to abiotic stresses in Salvia miltiorrhiza[J]. Plant Physiology and Biochemistry,2025,221:109669.
[24]赵陆滟,曹绍玉,龙云树,等. 全长转录组测序在植物中的应用研究进展[J]. 植物遗传资源学报,2019,20(6):1390-1398.
[25]陈默,陈璐,黄晓雅,等. PEG 模拟干旱胁迫下苗期木薯的生长生理响应[J]. 云南农业大学学报(自然科学),2024,39(4):15-22.
[26]王纪辉,侯娜,梁美. 水分胁迫下竹叶花椒的生理响应[J]. 江苏农业学报,2019,35(3):676-681.
[27]刘硕. PEG模拟干旱调控丹参酮生物合成的作用及机制[D]. 沈阳:沈阳农业大学,2024.
[28]周正,白玲,余燕,等. 持续水分胁迫对丹参茎叶酚酸含量及其抗氧化活性的影响[J]. 西北植物学报,2022,42(3):435-443.
[29]刘景玲. UV-B辐射和干旱对丹参生物量及酚酸类成分含量的影响[D]. 杨凌:西北农林科技大学,2014.

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备注/Memo

备注/Memo:
收稿日期：2025-02-18基金项目：安徽省高校优秀科研创新团队项目（2022AH010080）；宿州市科技计划重大专项（SZKJXM202423）；宿州学院非财政资金科研项目（2024xhx196）；宿州学院博士科研启动基金项目（2022BSK033）；宿州学院大学生创新创业训练计划项目（202510379040） 作者简介：刘发旺（1990-），男，安徽宿州人，博士，讲师，研究方向为中药化学和分子生物学。（E-mail）fawang10314@ahszu.edu.cn
更新日期/Last Update: 2025-12-18