[1]江伟,张晓,赵仁慧,等.基于全基因组关联分析挖掘小麦籽粒硬度指数和沉淀值的显著性位点[J].江苏农业学报,2025,(11):2088-2096.[doi:doi:10.3969/j.issn.1000-4440.2025.11.002]
　JIANG Wei,ZHANG Xiao,ZHAO Renhui,et al.Discovering of significant sites for wheat grain hardness and sedimentation value using genome-wide association study[J].,2025,(11):2088-2096.[doi:doi:10.3969/j.issn.1000-4440.2025.11.002]
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基于全基因组关联分析挖掘小麦籽粒硬度指数和沉淀值的显著性位点()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年11期
页码:
2088-2096
栏目:
遗传育种·生理生化
出版日期:
2025-11-30

文章信息/Info

Title:
Discovering of significant sites for wheat grain hardness and sedimentation value using genome-wide association study
作者:
江伟1; 2; 张晓2; 赵仁慧2; 李曼2; 吴旭江2; 李韬1; 胡文静2; 高德荣2
(1.扬州大学农学院,江苏扬州225007；2.江苏里下河地区农业科学研究所/农业农村部长江中下游小麦生物学与遗传育种重点实验室,江苏扬州225007)
Author(s):
JIANG Wei1; 2; ZHANG Xiao2; ZHAO Renhui2; LI Man2; WU Xujiang2; LI Tao1; HU Wenjing2; GAO Derong2
(1.Agricultural College of Yangzhou University, Yangzhou 225007, China;2.Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province/Key Laboratory of Wheat Biology and Genetic Breeding in the Middle and Lower Reaches of the Yangtze River, Ministry of Agriculture and Rural Affairs, Yangzhou 225007, China)
关键词:
小麦; 硬度指数; 沉淀值; 全基因组关联分析
Keywords:
wheat; grain hardness; sedimentation value; genome-wide association study (GWAS)
分类号:
S512.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.002
文献标志码:
A
摘要:
为解析小麦品质指标籽粒硬度指数(GH)与沉淀值(SV)的遗传基础,本研究利用扬麦16和扬麦22杂交获得F1、扬麦20和镇麦168杂交获得F1,再将2个单交组合的F1进行杂交产生四亲本杂交种,然后自交衍生获得320份稳定株系并将其作为试验材料。利用小麦15K SNP芯片数据,结合3年共4个环境的GH与SV表型数据,采用混合线性模型(MLM)进行全基因组关联分析。结果表明,GH表型分布呈现1∶1且在不同环境间具有显著相关性,SV表型符合正态分布且在不同环境间具有显著相关性。本研究共检测到8个与GH显著关联的位点,其中有4个位点为多环境稳定位点,分布在染色体5D(3个位点)和5A(1个位点)上,可解释5.80%～1340%的表型变异；检测到20个与SV显著关联的位点,其中3个位于染色体5D上的位点为多环境稳定位点,可解释4.78%～746%的表型变异；染色体5D上的AX-109986939、AX-111464164和AX-110476332位点同时与GH和SV显著关联,为一因多效稳定位点。进一步分析发现,AX-109986939、AX-111464164和AX-110476332对GH和SV均具有极显著效应(P＜001)。在染色体5A上的位点AX-110419834增效等位变异可使GH值提高2444%(P＜001)。
Abstract:
To elucidate the genetic basis of the grain quality indices including grain hardness (GH) and sedimentation value (SV) in wheat, this study utilized F1 hybrids from the crosses Yangmai 16 × Yangmai 22 and Yangmai 20 × Zhenmai 168. The F1 plants from these two single crosses were then hybridized to generate a four-parent cross, which was subsequently self-pollinated to derive 320 stable lines used as experimental materials. Using data from the wheat 15K SNP array and phenotypic data for GH and SV collected across four environments over three years, a genome-wide association study (GWAS) was conducted using a mixed linear model (MLM). The results showed that the GH phenotypic distribution exhibited a 1∶1 ratio and had significant correlations across different environments, while the SV phenotypic distribution followed a normal distribution and also showed significant correlations across environments. A total of eight loci significantly associated with GH were detected, four of which were stable across multiple environments. These stable loci were located on chromosomes 5D (three loci) and 5A (one locus), explaining 5.80% to 13.40% of the phenotypic variation. Twenty loci significantly asso-ciated with SV were detected, three of which on chromosome 5D were stable across multiple environments, explaining 478% to 7.46% of the phenotypic variation. The loci AX-109986939, AX-111464164, and AX-110476332 on chromosome 5D were significantly associated with both GH and SV, representing stable pleiotropic loci. Further analysis revealed that AX-109986939, AX-111464164, and AX-110476332 had highly significant effects (P＜001) on both GH and SV. The favorable allele variation at locus AX-110419834 on chromosome 5A increased the GH value by 24.44% (P＜001).

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备注/Memo

备注/Memo:
收稿日期：2025-02-12基金项目：国家小麦产业技术体系弱筋品种改良岗位项目(CARS-03-8)；农业生物育种重大专项(2023ZD0402504)；江苏里下河地区农业科学研究所科研发展专项[SJ(22)203]作者简介：江伟(1989-),男,安徽宣城人,硕士,助理研究员,研究方向为小麦品质育种。(E-mail)183036586@qq.com通讯作者：高德荣,(E-mail)gdr@wheat.org.cn；胡文静,(E-mail)huren2008@126.com
更新日期/Last Update: 2025-12-18