[1]王莹莹,刘照坤,王欢,等.不结球白菜萌发期耐盐性鉴定及综合评价[J].江苏农业学报,2025,(10):2027-2036.[doi:doi:10.3969/j.issn.1000-4440.2025.10.016]
　WANG Yingying,LIU Zhaokun,WANG Huan,et al.Identification and comprehensive evaluation of salt tolerance in non-heading Chinese cabbage at germination stage[J].,2025,(10):2027-2036.[doi:doi:10.3969/j.issn.1000-4440.2025.10.016]
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不结球白菜萌发期耐盐性鉴定及综合评价()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年10期
页码:
2027-2036
栏目:
园艺
出版日期:
2025-10-31

文章信息/Info

Title:
Identification and comprehensive evaluation of salt tolerance in non-heading Chinese cabbage at germination stage
作者:
王莹莹; 刘照坤; 王欢; 韩建军
(江苏太湖地区农业科学研究所,江苏苏州215000)
Author(s):
WANG Yingying; LIU Zhaokun; WANG Huan; HAN Jianjun
(Jiangsu Taihu Lake Region Institute of Agricultural Sciences, Suzhou 215000, China)
关键词:
不结球白菜; 萌发期; 耐盐性; 综合评价
Keywords:
non-heading Chinese cabbage; germination stage; salt tolerance; comprehensive evaluation
分类号:
S634.3
DOI:
doi:10.3969/j.issn.1000-4440.2025.10.016
文献标志码:
A
摘要:
加强作物种质资源的耐盐性鉴定与耐盐新品种培育对盐渍化土壤利用和农业发展具有重要意义。为鉴定不结球白菜种质资源萌发期的耐盐性,本研究在筛选不结球白菜种质萌发期耐盐性适宜盐浓度的基础上,对江苏太湖地区农业科学研究所培育或收集的48份不结球白菜种质进行蒸馏水对照(CK)和适宜盐浓度的胁迫处理,得到萌发期盐胁迫下的相对生长指标(盐胁迫处理与CK的生长指标比值)。基于主成分分析、隶属函数分析等方法,综合评价供试种质的耐盐性,建立不结球白菜萌发期耐盐性综合评价体系。结果表明,不结球白菜种质萌发期耐盐性鉴定的适宜盐浓度为200 mmol/L,该浓度盐胁迫下,不结球白菜发芽势、发芽率、发芽指数、活力指数、芽长、根长、根鲜重、根冠比等生长指标均显著低于CK；多对相对生长指标(耐盐系数)存在显著或极显著的相关性。主成分分析结果显示,10个相对生长指标(相对发芽势、相对发芽率、相对发芽指数、相对活力指数、相对芽长、相对根长、相对芽鲜重、相对根鲜重、相对根冠比、相对盐害率)可降维为3个主成分,其累计方差贡献率达到85.72%。根据耐盐性综合指标D值,可将48份种质聚类为5个耐盐等级,包括高耐盐型种质2份、中耐盐型种质21份、低耐盐型种质12份、盐敏感型种质9份、高盐敏感型种质4份。逐步回归分析结果表明,相对根长、相对根鲜重、相对根冠比、相对活力指数、相对盐害率和相对芽长等6项指标是不结球白菜萌发期耐盐性鉴定的关键因子。
Abstract:
It is of great significance to strengthen the identification of salt tolerance of crop germplasm resources and the cultivation of new salt-tolerant varieties for the utilization of salinized soil and agricultural development. In order to identify the salt tolerance of non-heading Chinese cabbage germplasm resources during germination stage, based on the screening of suitable salt concentration for salt tolerance of non-heading Chinese cabbage germplasm during germination stage, 48 non-heading Chinese cabbage germplasms cultivated or collected by Jiangsu Taihu Lake Region Institute of Agricultural Sciences were subjected to distilled water control (CK) and stress treatment with suitable salt concentration, and the relative growth index (the ratio of growth index under salt stress treatment to that of CK) under salt stress during germination stage was obtained. Based on principal component analysis, membership function analysis and other methods, the salt tolerance of the tested germplasm was comprehensively evaluated, and a comprehensive evaluation system for salt tolerance of non-heading Chinese cabbage at germination stage was established. The results showed that the suitable salt concentration for salt tolerance identification of non-heading Chinese cabbage germplasm at germination stage was 200 mmol/L. Under this concentration of salt stress, the germination potential, germination rate, germination index, vigor index, shoot length, root length, root fresh weight and root-shoot ratio of non-heading Chinese cabbage were significantly lower than those of CK. There were significant or extremely significant correlations between multiple pairs of relative growth indexes (salt tolerance coefficient). Principal component analysis showed that ten relative growth indexes (relative germination potential, relative germination rate, relative germination index, relative vigor index, relative shoot length, relative root length, relative shoot fresh weight, relative root fresh weight, relative root-shoot ratio, relative salt damage rate) could be reduced to three principal components, and the cumulative variance contribution rate reached 85.72%. According to the comprehensive index D value of salt tolerance, 48 germplasms could be clustered into five salt tolerance grades, including two high salt tolerant germplasms, 21 medium salt tolerant germplasms, 12 low salt tolerant germplasms, nine salt sensitive germplasms and four high salt sensitive germplasms. Stepwise regression analysis showed that relative root length, relative root fresh weight, relative root-shoot ratio, relative vigor index, relative salt damage rate and relative shoot length were the key factors for salt tolerance identification of non-heading Chinese cabbage at germination stage.

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备注/Memo

备注/Memo:
收稿日期：2025-07-09基金项目：江苏省省级农业种质资源保护项目(JS-ZW-K18)；苏州市农业科学院科研基金项目(24028)作者简介：王莹莹(1998-),女,河南兰考人,硕士,研究实习员,主要从事不结球白菜种质资源创新研究。(E-mail)wangyingying@jaas.ac.cn通讯作者：刘照坤,(E-mail)saaslzk@qq.com
更新日期/Last Update: 2025-11-17