[1]简子林,王自力,秦畅,等.小麦TGA转录因子家族全基因组鉴定及其响应非生物胁迫的表达分析[J].江苏农业学报,2025,(10):1873-1885.[doi:doi:10.3969/j.issn.1000-4440.2025.10.001]
 JIAN Zilin,WANG Zili,QIN Chang,et al.Genome-wide identification of the TGA transcription factor family and expression analysis under abiotic stress in Triticum aestivum L.[J].,2025,(10):1873-1885.[doi:doi:10.3969/j.issn.1000-4440.2025.10.001]
点击复制

小麦TGA转录因子家族全基因组鉴定及其响应非生物胁迫的表达分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年10期
页码:
1873-1885
栏目:
遗传育种·生理生化
出版日期:
2025-10-31

文章信息/Info

Title:
Genome-wide identification of the TGA transcription factor family and expression analysis under abiotic stress in Triticum aestivum L.
作者:
简子林王自力秦畅何方任明见
(贵州大学农学院,贵州贵阳550025)
Author(s):
JIAN ZilinWANG ZiliQIN ChangHE FangREN Mingjian
(College of Agriculture, Guizhou University, Guiyang 550025, China)
关键词:
小麦TGA转录因子生物信息学表达分析
Keywords:
wheatTGAtranscription factorsbioinformaticsexpression analysis
分类号:
S512.101
DOI:
doi:10.3969/j.issn.1000-4440.2025.10.001
文献标志码:
A
摘要:
TGA转录因子属于碱性亮氨酸拉链(bZIP)家族的D亚组,在植物对病原体的防御、植物生长发育、植物应对不同逆境胁迫等多种生物进程和信号转导过程中发挥重要功能。本研究利用小麦全基因组数据鉴定小麦TGA转录因子家族,分析其基因结构、保守结构域、顺式作用元件和系统发育关系等多类生物信息学特性,并用实时荧光定量PCR(qRT-PCR)技术探究部分小麦TGA转录因子基因在不同组织中和不同胁迫处理下的表达模式。结果表明,共鉴定出38个不均匀地分布在小麦21条染色体上的TaTGA转录因子基因,通过系统发育分析,将这些TaTGA分为5个亚家族,各亚家族内TaTGA转录因子基因的基因结构和保守基序及保守结构域的特征基本一致。顺式作用元件分析结果表明,TaTGA包含多种类型的顺式作用元件,涵盖植物生长发育、对非生物胁迫的响应、激素作用等不同功能区域。qRT-PCR结果显示,TaTGA转录因子基因的相对表达量不同程度地受到逆境胁迫及激素作用的影响。本研究结果为小麦抗逆性分子育种提供了有益候选基因,并为进一步研究TGA转录因子在植物中的功能奠定了基础。
Abstract:
TGA transcription factors belong to subgroup D of the basic leucine zipper (bZIP) family and are widely involved in various biological processes and signal transduction pathways, playing important roles in plant defense against pathogens, plant growth and development, and responses to different abiotic stresses. In this study, we identified the wheat TGA transcription factor family using whole-genome data of wheat and analyzed various bioinformatics characteristics, including gene structures, conserved domains, cis-acting elements, and phylogenetic relationships. Additionally, we explored the expression patterns of some wheat TGA transcription factor genes in different tissues and under various stress treatments using quantitative real-time PCR (qRT-PCR). A total of 38 TaTGA transcription factor genes were identified, which were unevenly distributed across the 21 chromosomes of wheat. Phylogenetic analysis classified these TaTGA transcription factors into five subfamilies. Within each subfamily, the gene structures, conserved motifs, and conserved domains of the TaTGA transcription factors were essentially consistent. The analysis of cis-acting elements revealed that TaTGA genes contain various types of cis-acting elements, covering functional regions related to plant growth and development, responses to abiotic stresses, and hormone actions. The qRT-PCR results indicated that the relative expression levels of TaTGA genes were differentially affected by abiotic stresses and hormone treatments. The findings of this study provide valuable candidate genes for molecular breeding of stress-resistant wheat and lay a foundation for further research on the functions of TGA transcription factors in plants.

参考文献/References:

[1]ARNAUD D, HWANG I. A sophisticated network of signaling pathways regulates stomatal defenses to bacterial pathogens[J]. Molecular Plant,2015,8(4):566-581.
[2]QI J S, SONG C P, WANG B S, et al. Reactive oxygen species signaling and stomatal movement in plant responses to drought stress and pathogen attack[J]. Journal of Integrative Plant Biology,2018,60(9):805-826.
[3]BAILLO E H, KIMOTHO R N, ZHANG Z B, et al. Transcription factors associated with abiotic and biotic stress tolerance and their potential for crops improvement[J]. Genes,2019,10(10):771.
[4]SINGH K B, FOLEY R C, OATE-SNCHEZ L. Transcription factors in plant defense and stress responses[J]. Current Opinion in Plant Biology,2002,5(5):430-436.
[5]SORNARAJ P, LUANG S, LOPATO S, et al. Basic leucine zipper (bZIP) transcription factors involved in abiotic stresses:a molecular model of a wheat bZIP factor and implications of its structure in function[J]. Biochimica et Biophysica Acta-General Subjects,2016,1860(1):46-56.
[6]AGARWAL P, BARANWAL V K, KHURANA P. Genome-wide analysis of bZIP transcription factors in wheat and functional characterization of a TabZIP under abiotic stress[J]. Scientific Reports,2019,9(1):4608.
[7]JAKOBY M, WEISSHAAR B, DRGE-LASER W, et al. bZIP transcription factors in Arabidopsis[J]. Trends in Plant Science,2002,7(3):106-111.
[8]LU C F, LIU X Y, TANG Y Q, et al. A comprehensive review of TGA transcription factors in plant growth,stress responses,and beyond[J]. International Journal of Biological Macromolecules,2024,258:128880.
[9]NIJHAWAN A, JAIN M, TYAGI A K, et al. Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice[J]. Plant Physiology,2008,146(2):333-350.
[10]MAGNANI E, DE KLEIN N, NAM H I, et al. A comprehensive analysis of microProteins reveals their potentially widespread mechanism of transcriptional regulation[J]. Plant Physiology,2014,165(1):149-159.
[11]GATZ C. From pioneers to team players:TGA transcription factors provide a molecular link between different stress pathways[J]. Molecular Plant-Microbe Interactions,2013,26(2):151-159.
[12]IDROVO ESPN F M, PERAZA-ECHEVERRIA S, FUENTES G, et al. In silico cloning and characterization of the tga (tgacg motif-binding factor) transcription factors subfamily in Carica papaya[J]. Plant Physiology and Biochemistry,2012,54:113-122.
[13]徐东东,东琳,邵丽,等. TGA转录因子在调控植物逆境应答和生长发育中的作用研究进展[J]. 植物生理学报,2024,60(7):1079-1086.
[14]田义,张彩霞,康国栋,等. 植物TGA转录因子研究进展[J]. 中国农业科学,2016,49(4):632-642.
[15]XIANG C, MIAO Z H, LAM E. Coordinated activation of as-1-type elements and a tobacco glutathione S-transferase gene by auxins,salicylic acid,methyl-jasmonate and hydrogen peroxide[J]. Plant Molecular Biology,1996,32(3):415-426.
[16]NIGGEWEG R, THUROW C, KEGLER C, et al. Tobacco transcription factor TGA2.2 is the main component of as-1-binding factor ASF-1 and is involved in salicylic acid- and auxin-inducible expression of as-1-containing target promoters[J]. Journal of Biological Chemistry,2000,275(26):19897-19905.
[17]KESARWANI M, YOO J, DONG X N. Genetic interactions of TGA transcription factors in the regulation of pathogenesis-related genes and disease resistance in Arabidopsis[J]. Plant Physiology,2007,144(1):336-346.
[18]MURMU J, BUSH M J, DELONG C, et al. Arabidopsis basic leucine-zipper transcription factors TGA9 and TGA10 interact with floral glutaredoxins ROXY1 and ROXY2 and are redundantly required for anther development[J]. Plant Physiology,2010,154(3):1492-1504.
[19]ZHONG L, CHEN D D, MIN D H, et al. AtTGA4,a bZIP transcription factor,confers drought resistance by enhancing nitrate transport and assimilation in Arabidopsis thaliana[J]. Biochemical and Biophysical Research Communications,2015,457(3):433-439.
[20]DUAN Y, XU Z S, LIU H, et al. Genome-wide identification of the TGA gene family and expression analysis under drought stress in Brassica napus L.[J]. International Journal of Molecular Sciences,2024,25(12):6355.
[21]KE D, HE Y, FAN L, et al. The soybean TGA transcription factor GmTGA13 plays important roles in the response to salinity stress[J]. Plant Biology,2022,24(2):313-322.
[22]LI B, LIU Y, CUI X Y, et al. Genome-wide characterization and expression analysis of soybean TGA transcription factors identified a novel TGA gene involved in drought and salt tolerance[J]. Frontiers in Plant Science,2019,10:549.
[23]GUPTA P K, BALYAN H S, SHARMA S, et al. Genetics of yield,abiotic stress tolerance and biofortification in wheat (Triticum aestivum L. )[J]. Theoretical and Applied Genetics,2020,133(5):1569-1602.
[24]SONG L, LI W T, CHEN X W. Transcription factor is not just a transcription factor[J]. Trends in Plant Science,2022,27(11):1087-1089.
[25]HRMOVA M, HUSSAIN S S. Plant transcription factors involved in drought and associated stresses[J]. International Journal of Molecular Sciences,2021,22(11):5662.
[26]JAVED T, SHABBIR R, ALI A, et al. Transcription factors in plant stress responses:challenges and potential for sugarcane improvement[J]. Plants,2020,9(4):491.
[27]LIU T L, CHEN T Z, KAN J L, et al. The GhMYB36 transcription factor confers resistance to biotic and abiotic stress by enhancing PR1 gene expression in plants[J]. Plant Biotechnology Journal,2022,20(4):722-735.
[28]ZHANG Y, XIA P G. The DREB transcription factor,a biomacromolecule,responds to abiotic stress by regulating the expression of stress-related genes[J]. International Journal of Biological Macromolecules,2023,243:125231.
[29]WANG X P, NIU Y L, ZHENG Y. Multiple functions of MYB transcription factors in abiotic stress responses[J]. International Journal of Molecular Sciences,2021,22(11):6125.
[30]ZHANG H, LIU S J, REN T M, et al. Crucial abiotic stress regulatory network of NF-Y transcription factor in plants[J]. International Journal of Molecular Sciences,2023,24(5):4426.
[31]孟新超,殷恒梅,张兴政,等. 玉米TGA转录因子家族全基因组鉴定及盐胁迫相关表达分析[J]. 江西农业大学学报,2023,45(3):631-642.
[32]LAM E, LAM Y K. Binding site requirements and differential representation of TGF factors in nuclear ASF-1 activity[J]. Nucleic Acids Research,1995,23(18):3778-3785.
[33]JIA J L, QING D Z, DE J H, et al. Genome-wide identification of TGA transcription factors in wheat:evolution, expression and verification[EB/OL]. Research Square, 2023
[2024-10-01]. https://doi. org/10. 21203/rs. 3. rs-2693252/v1].

相似文献/References:

[1]伍 宏,朱昌华,夏 凯,等.叶面喷施激动素对小麦品种济麦22品质的影响[J].江苏农业学报,2016,(02):299.[doi:10.3969/j.issn.1000-4440.2016.02.010]
 WU Hong,ZHU Chang-hua,XIA Kai,et al.Effect of foliar application of kinetin on quality of Triticum aestivum L. Jimai 22[J].,2016,(10):299.[doi:10.3969/j.issn.1000-4440.2016.02.010]
[2]蒋正宁,别同德,赵仁惠,等.受条锈菌诱导的小麦丝氨酸苏氨酸激酶基因TaS/TK的克隆与表达[J].江苏农业学报,2016,(05):980.[doi:10.3969/j.issn.1000-4440.2016.05.004]
 JIANG Zheng-ning,BIE Tong-de,ZHAO Ren-hui,et al.Cloning and expression analysis of a Serine/Threonine protein kinase gene TaS/TK in wheat in response to stripe rust fungal infection[J].,2016,(10):980.[doi:10.3969/j.issn.1000-4440.2016.05.004]
[3]丁彬彬,张旭,吴磊,等.小麦3B 短臂染色体抗赤霉病主效 QTL 区域候选基因的表达[J].江苏农业学报,2017,(01):6.[doi:10.3969/j.issn.1000-4440.2017.01.002 ]
 DING Bin-bin,ZHANG Xu,WU Lei,et al.Expression of candidate genes on the region of a major QTL for the resistance to Fusarium head blight on the short arm of chromosome 3B in wheat[J].,2017,(10):6.[doi:10.3969/j.issn.1000-4440.2017.01.002 ]
[4]周淼平,姚金保,张鹏,等.小麦幼苗纹枯病抗性评价新方法[J].江苏农业学报,2017,(01):61.[doi:10.3969/j.issn.1000-4440.2017.01.010 ]
 ZHOU Miao-ping,YAO Jin-bao,ZHANG Peng,et al.New method for the resistance evaluation of wheat sharp eyespot in seedling[J].,2017,(10):61.[doi:10.3969/j.issn.1000-4440.2017.01.010 ]
[5]吴磊,姜朋,张瑜,等.苏麦3号小麦穗部病毒诱导的基因沉默(VIGS)体系的建立及验证[J].江苏农业学报,2017,(02):248.[doi:doi:10.3969/j.issn.1000-4440.2017.02.002]
 WU Lei,JIANG Peng,ZHANG Yu,et al.Construction and validation of virus-induced gene silencing(VIGS) system in spike of wheat variety Sumai 3[J].,2017,(10):248.[doi:doi:10.3969/j.issn.1000-4440.2017.02.002]
[6]邵继锋,陈荣府,董晓英,等.利用分根技术研究小麦铝磷交互作用[J].江苏农业学报,2016,(01):78.[doi:10.3969/j.issn.1000-4440.2016.01.012 ]
 SHAO Ji-feng,CHEN Rong-fu,DONG Xiao-ying,et al.Aluminum-phosphorus interaction in wheat grown in a split-root device[J].,2016,(10):78.[doi:10.3969/j.issn.1000-4440.2016.01.012 ]
[7]叶景秀.小麦籽粒蛋白质双向电泳体系的优化[J].江苏农业学报,2015,(05):957.[doi:doi:10.3969/j.issn.1000-4440.2015.05.002]
 YE Jing-xiu.Optimization of two-dimensional electrophresis system for grain protein in spring wheat[J].,2015,(10):957.[doi:doi:10.3969/j.issn.1000-4440.2015.05.002]
[8]郑舒文,徐其隆,邹华文.脱落酸对涝渍胁迫下小麦产量的影响[J].江苏农业学报,2015,(05):967.[doi:doi:10.3969/j.issn.1000-4440.2015.05.004]
 ZHENG Shu-wen,XU Qi-long,ZOU Hua-wen.Yield of waterlogged wheat in response to ABA application[J].,2015,(10):967.[doi:doi:10.3969/j.issn.1000-4440.2015.05.004]
[9]张玉萍,马占鸿.不同施氮量下小麦遥感估产模型构建[J].江苏农业学报,2015,(06):1325.[doi:doi:10.3969/j.issn.1000-4440.2015.06.020]
 ZHANG Yu-ping,MA Zhan-hong.Yield estimation model of wheat based on remote sensing data under different nitrogen supply conditions[J].,2015,(10):1325.[doi:doi:10.3969/j.issn.1000-4440.2015.06.020]
[10]张卓亚,王晓琳,许晓明,等.腐植酸对小麦扬花期水分利用效率及灌浆进程的影响[J].江苏农业学报,2015,(04):725.[doi:10.3969/j.issn.1000-4440.2015.04.003]
 ZHANG Zhuo-ya,WANG Xiao-ling,XU Xiao-ming,et al.Effect of humic acid on water use efficiency and grouting process of wheat at flowering[J].,2015,(10):725.[doi:10.3969/j.issn.1000-4440.2015.04.003]

备注/Memo

备注/Memo:
收稿日期:2024-10-15基金项目:国家自然科学基金项目(32260481);贵州省科技计划项目[黔科合支撑(2022)重点026];贵州省粮油作物分子育种重点实验室项目[黔科合中引地(2023)008];贵州省高等学校功能农业重点实验室项目[黔教技(2023)007号]作者简介:简子林(2001-),男,贵州贵阳人,硕士研究生,主要从事小麦育种研究。(E-mail)1395488225@qq.com通讯作者:任明见,(E-mail)rmj72@163.com
更新日期/Last Update: 2025-11-17