[1]杨爱清,杨小艺,徐辰武,等.低温胁迫下玉米根系转录组和代谢组分析[J].江苏农业学报,2025,(06):1063-1071.[doi:doi:10.3969/j.issn.1000-4440.2025.06.003]
　YANG Aiqing,YANG Xiaoyi,XU Chenwu,et al.Transcriptome and metabolome analysis of maize roots under low temperature stress[J].,2025,(06):1063-1071.[doi:doi:10.3969/j.issn.1000-4440.2025.06.003]
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低温胁迫下玉米根系转录组和代谢组分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年06期
页码:
1063-1071
栏目:
遗传育种·生理生化
出版日期:
2025-06-30

文章信息/Info

Title:
Transcriptome and metabolome analysis of maize roots under low temperature stress
作者:
杨爱清; 杨小艺; 徐辰武; 李鹏程; 王芸芸; 王后苗
(江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室/江苏省作物基因组学和分子育种重点实验室/江苏省粮食作物现代产业技术协同创新中心/扬州大学农学院,江苏扬州225009)
Author(s):
YANG Aiqing; YANG Xiaoyi; XU Chenwu; LI Pengcheng; WANG Yunyun; WANG Houmiao
(Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Agricultural College of Yangzhou University, Yangzhou 225009, China)
关键词:
玉米; 低温胁迫; 转录组学; 代谢组学
Keywords:
maize; low temperature stress; transcriptomics; metabolomics
分类号:
S513.01
DOI:
doi:10.3969/j.issn.1000-4440.2025.06.003
文献标志码:
A
摘要:
低温是限制玉米苗期生长的主要非生物胁迫之一。为了明确低温胁迫对玉米根系生长影响的分子机制,本研究以玉米自交系B73为试验材料,对常温对照(白天温度28 ℃，夜间温度22 ℃,CK)和低温处理(白天温度15 ℃，夜间温度10 ℃)后不同时间点的主胚根生长进行动态监测,并对CK和低温处理1 d根系组织进行转录组和代谢组分析。结果表明,在低温胁迫下,玉米主胚根生长受到明显抑制。CK和低温处理根系代谢组中共鉴定到57种差异代谢物,这些代谢物主要参与淀粉和蔗糖代谢。CK和低温处理根系转录组分析共鉴定到2 769个差异表达基因,主要参与淀粉和蔗糖代谢、苯丙氨酸代谢。淀粉和蔗糖代谢是介导根系适应低温胁迫的关键通路。低温胁迫下,玉米根系中果糖和葡萄糖等可溶性糖含量显著增加。在淀粉和蔗糖代谢通路中共鉴定到26个差异表达基因,低温胁迫下12个基因表达水平显著上调,14个基因表达水平显著下调。本研究结果为进一步探索玉米根系适应低温胁迫的分子机制和品种遗传改良提供了基础和候选基因。
Abstract:
Cold stress is one of the primary abiotic stresses limiting the growth of maize seedlings. In order to study the effect of low temperature stress on maize roots, this study used maize inbred line B73 as the experimental material, and dynamically monitored the growth of the primary root at different time points under control (28 ℃ day/22 ℃ night) and low-temperature treatment (15 ℃ day/10 ℃ night). Additionally, we determined the transcriptome and metabolome of the root tissues from both the control group and the 1-day low-temperature treatment group. Phenotypic characterization results indicated that the growth of the primary root was significantly inhibited under low-temperature stress. Metabolomic analysis identified 57 differentially accumulated metabolites, predominantly enriched in starch and sucrose metabolism pathways. A total of 2 769 differentially expressed genes were identified by transcriptome analysis, which were mainly involved in starch and sucrose metabolism and phenylalanine metabolism. Further integration of transcriptomic and metabolomic analyses revealed that starch and sucrose metabolism were key pathways mediating root adaptation to low-temperature stress. Under low-temperature stress, the content of soluble sugars such as fructose and glucose significantly increased. A total of 26 differentially expressed genes were identified in the starch and sucrose metabolic pathways, with 12 genes showing significant upregulation and 14 genes showing significant downregulation. This study provides a theoretical foundation and candidate genes for further investigation into the molecular mechanisms of maize root adaptation to low-temperature stress and for genetic improvement.

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备注/Memo

备注/Memo:
收稿日期：2024-11-13基金项目：国家自然科学基金项目(32302654)；江苏省重点研发项目(BE2022343)作者简介：杨爱清(1999-),女,山东德州人,硕士研究生,主要从事玉米遗传和分子育种研究。(E-mail)mz120221390@stu.yzu.edu.cn通讯作者：王后苗,(E-mail)houmiaowang@yzu.edu.cn
更新日期/Last Update: 2025-07-16