[1]胡飞,童友敏,徐婷婷,等.球孢白僵菌Bb378不同施用方式对大豆促生及诱导抗甜菜夜蛾的增效作用[J].江苏农业学报,2025,(04):656-663.[doi:doi:10.3969/j.issn.1000-4440.2025.04.004]
　HU Fei,TONG Youmin,XU Tingting,et al.Enhancement of soybean growth and resistance to Spodoptera exigua by Beauveria bassiana Bb378 through different application methods[J].,2025,(04):656-663.[doi:doi:10.3969/j.issn.1000-4440.2025.04.004]
点击复制

球孢白僵菌Bb378不同施用方式对大豆促生及诱导抗甜菜夜蛾的增效作用()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年04期
页码:
656-663
栏目:
植物保护
出版日期:
2025-04-30

文章信息/Info

Title:
Enhancement of soybean growth and resistance to Spodoptera exigua by Beauveria bassiana Bb378 through different application methods
作者:
胡飞1; 童友敏1; 徐婷婷1; 彭辉2; 胡本进1; 苏贤岩1; 毕思佳1; 吴振晨1; 徐丽娜1
(1.安徽省农业科学院植物保护与农产品质量安全研究所,安徽合肥230031；2.安徽农业大学,安徽合肥230036)
Author(s):
HU Fei1; TONG Youmin1; XU Tingting1; PENG Hui2; HU Benjin1; SU Xianyan1; BI Sijia1; WU Zhenchen1; XU Lina1
(1.Institute of Plant Protection and Agro-products Quality Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China;2.Anhui Agricultural University, Hefei 230036, China)
关键词:
球孢白僵菌; 大豆; 促生长; 驱避; 甜菜夜蛾
Keywords:
Beauveria bassiana; soybean; growth-promoting; repellant; Spodoptera exigua
分类号:
S476
DOI:
doi:10.3969/j.issn.1000-4440.2025.04.004
文献标志码:
A
摘要:
为研究球孢白僵菌Bb378对大豆生长的影响及对大豆抵御甜菜夜蛾危害的作用,本研究以1株对甜菜夜蛾具有杀虫活性的球孢白僵菌Bb378为研究对象,采用浸种处理、灌根处理和叶面喷施处理研究该菌株对大豆的促生作用及其与大豆共生对甜菜夜蛾取食行为的影响。结果表明,Bb378处理甜菜夜蛾3龄幼虫14 d后的累计校正死亡率为56.92%,LC50为3.7×107CFU/mL；球孢白僵菌Bb378孢悬液含量为1×108CFU/mL时,浸种处理和灌根处理对大豆株高、叶面积和植株鲜重促生效果明显,部分与对照相比显著增加(P＜0.05)；而球孢白僵菌Bb378孢悬液含量分别为1×107CFU/mL和1×106CFU/mL时,叶面喷施处理分别对大豆叶面积（齐苗后第21 d）和植株鲜重影响最大。此外,Bb378含量为1×108CFU/mL和1×107CFU/mL时,浸种处理、灌根处理和叶面喷施处理整体上对甜菜夜蛾初孵幼虫有良好的驱避效果,而浸种处理和灌根处理对甜菜夜蛾3龄幼虫驱避效果显著。本研究结果表明,球孢白僵菌Bb378接种后可以促进大豆生长,且球孢白僵菌-大豆共生体对甜菜夜蛾具有驱避效果,为球孢白僵菌类杀虫剂的田间应用提供了技术支撑。
Abstract:
To study the effects of Beauveria bassiana Bb378 on the growth of soybean and its resistance to Spodoptera exigua, a B. bassiana strain Bb378 with insecticidal activity against S. exigua was used to investigate its growth promotion effect on soybean and the effects of its symbiotic relationship with soybean on the feeding behavior of S. exigua by seed soaking, root irrigation and foliar spraying methods. The results showed that the cumulative corrected mortality of the third instar larvae was 56.92% after 14 days of Bb378 treatment, and the LC50 was 3.7×107CFU/mL. When the content of Bb378 spore suspension was 1×108CFU/mL, seed soaking and root irrigation treatments had obvious effects on plant height, leaf area and fresh weight of soybean, part of which were significant increased compared with the control. When the suspension contents of Bb378 were 1×107CFU/mL and 1×106CFU/mL, respectively, foliar spraying had the greatest effect on leaf area (the twenty-first day after the seedlings were aligned) and fresh weight of soybean, respectively. In addition, when the contents of Bb378 were 1×108CFU/mL and 1×107CFU/mL, treatments of seed soaking, root irrigation and foliar spraying had good repellent effect on the newly hatched larvae of S. exigua on the whole, while treatments of seed soaking and root irrigation had significant repellent effect on the third instar larvae of S. exigua. The results showed that B. bassiana Bb378 could promote soybean growth after inoculation, and the B. bassiana-soybean symbiont had a repellant effect on S. exigua, which provided technical support for the further application of B. bassiana insecticide in the field.

参考文献/References:

[1]农向群,王广君,王以燕,等. 生物农药白僵菌杀虫剂的研发进展[J]. 植物保护学报,2024,51(2):278-293.
[2]李增智. 我国利用真菌防治害虫的历史、进展及现状[J]. 中国生物防治学报,2015,31(5):699-711.
[3]黄震,刘思,农向群,等. 防控红火蚁球孢白僵菌饵剂的载体饵料与引诱剂筛选[J/OL]. 中国生物防治学报,2024:1-15
[2024-08-30]. https://link.cnki.net/doi/10.16409/j.cnki.2095-039x.2024.03.013.
[4]谢敏,路杨,张云月,等. 球孢白僵菌-玉米共生体对亚洲玉米螟个体发育的影响及其机理[J/OL]. 应用昆虫学报,2024:1-9
[2024-08-30].https://link.cnki.net/urlid/11.6020.Q.20240322.1533.034.
[5]隋丽,徐文静,朱慧,等. 球孢白僵菌-玉米共生体对亚洲玉米螟取食行为的影响[J]. 中国生物防治学报,2020,36(1):46-51.
[6]刘晓丽,张鑫鑫,于军华,等. 白僵菌在大豆植株中的内生性及对八字地老虎幼虫杀虫活性研究[J]. 东北农业大学学报,2024,55(1):14-22.
[7]HAFEEZ M, ULLAH F, KHAN M M, et al. Metabolic-based insecticide resistance mechanism and ecofriendly approaches for controlling of beet armyworm Spodoptera exigua:a review[J]. Environmental Science and Pollution Research International,2022,29(2):1746-1762.
[8]CHE W N, SHI T, WU Y D, et al. Insecticide resistance status of field populations of Spodoptera exigua (Lepidoptera:Noctuidae) from China[J]. Journal of Economic Entomology,2013,106(4):1855-1862.
[9]SU J Y, SUN X X. High level of metaflumizone resistance and multiple insecticide resistance in field populations of Spodoptera exigua (Lepidoptera:Noctuidae) in Guangdong province,China[J]. Crop Protection,2014,61:58-63.
[10]WANG P, YANG F, WANG Y, et al. Monitoring the resistance of the beet armyworm (Lepidoptera:Noctuidae) to four insecticides in Southern China from 2014 to 2018[J]. Journal of Economic Entomology,2021,114(1):332-338.
[11]WANG X G, XIANG X, YU H L, et al. Monitoring and biochemical characterization of beta-cypermethrin resistance in Spodoptera exigua (Lepidoptera:Noctuidae) in Sichuan province,China[J]. Pesticide Biochemistry and Physiology,2018,146:71-79.
[12]周利琳,熊凯凡,王攀,等. 不同地区甜菜夜蛾的抗药性及田间防效[J]. 中国蔬菜,2023(7):84-91.
[13]毕思佳,吴晨源,胡本进,等. 球孢白僵菌Bb378菌株的分离、鉴定及对草地贪夜蛾的致病性研究[J]. 应用昆虫学报,2023,60(4):1223-1232.
[14]陈其津,李广宏,庞义. 饲养五种夜蛾科昆虫的一种简易人工饲料[J]. 昆虫知识,2000,37(6):325-327.
[15]吕利华,冯明光. 布氏白僵菌昆虫寄主名录[J]. 中国生物防治,1995,11(3):144-145.
[16] 张燕南,毕司进,李悦, 等. 球孢白僵菌菌株GZGY对茶黄螨致病力及生长发育的影响[J]. 应用昆虫学报,2023,60(6):1835-1840.
[17]马润平,马玲,苏光祥,等. 球孢白僵菌对榛实象成虫毒力及解毒酶的影响[J]. 东北林业大学学报,2024,52(7):125-129.
[18]付楠霞,罗宗秀,李兆群,等. 一株球孢白僵菌的分离鉴定及其对茶丽纹象甲幼虫的室内致病力测定[J]. 中国生物防治学报,2023,39(5):1104-1112.
[19]JABER L R, ENKERLI J. Effect of seed treatment duration on growth and colonization of Vicia faba by endophytic Beauveria bassiana and Metarhizium brunneum[J]. Biological Control,2016,103:187-195.
[20]AFANDHI A, WIDJAYANTI T, EMI A A L, et al. Endophytic fungi Beauveria bassiana Balsamo accelerates growth of common bean (Phaeseolus vulgaris L.)[J]. Chemical and Biological Technologies in Agriculture,2019,6(1):11.
[21]张涵飞,李雪兵,周建如,等. 接种球孢白僵菌提高烟草对蚜虫和白粉菌的抗性[J]. 植物医生,2018(11):49-52.
[22]隋丽,路杨,谢敏,等. 亚洲玉米螟对球孢白僵菌分生孢子和芽生孢子-玉米共生体的取食选择和嗅觉反应[J]. 昆虫学报,2023,66(11):1482-1489.

相似文献/References:

[1]刘朝茂,李成云.玉米与大豆间作对玉米叶片衰老的影响[J].江苏农业学报,2017,(02):322.[doi:doi:10.3969/j.issn.1000-4440.2017.02.013]
　LIU Chao-mao,LI Cheng-yun.Effects of maize/soybean intercropping on maize leaf senescence[J].,2017,(04):322.[doi:doi:10.3969/j.issn.1000-4440.2017.02.013]
[2]张令瑄,谢婷婷,王瑾,等.大田条件下ＵＶ-Ｂ 辐射增强对大豆根际土壤相关指标的影响[J].江苏农业学报,2016,(01):118.[doi:10.3969/j.issn.1000-4440.2016.01.018]
　ZHANG Ling-xuan,XIE Ting-ting,WANG Jin,et al.Soybean rhizosphere soil parameters in response to enhanced UV-B radiation under field condition[J].,2016,(04):118.[doi:10.3969/j.issn.1000-4440.2016.01.018]
[3]宁丽华,何晓兰,张大勇.大豆耐盐相关基因GmNcl1功能标记的开发及验证[J].江苏农业学报,2017,(06):1227.[doi:doi:10.3969/j.issn.1000-4440.2017.06.005]
　NING Li-hua,HE Xiao-lan,ZHANG Da-yong.Development and validation of the function marker of soybean salt tolerance gene GmNcl1[J].,2017,(04):1227.[doi:doi:10.3969/j.issn.1000-4440.2017.06.005]
[4]杨艳丽,杨勇,李大红,等.转桃PpCuZnSOD基因大豆的耐旱性[J].江苏农业学报,2018,(05):978.[doi:doi:10.3969/j.issn.1000-4440.2018.05.003]
　YANG Yan-li,YANG Yong,LI Da-hong,et al.Drought tolerance of transgenic soybean with PpCuZnSOD gene[J].,2018,(04):978.[doi:doi:10.3969/j.issn.1000-4440.2018.05.003]
[5]孙彦坤,陈睿,李静,等.不同降雨年型下反枝苋和大豆光合特征的比较[J].江苏农业学报,2019,(03):554.[doi:doi:10.3969/j.issn.1000-4440.2019.03.008]
　SUN Yan-kun,CHEN Rui,LI Jing,et al.Comparison of photosynthetic characteristics between Amaranthus retroexus and Glycine max under different annual rainfall pattern[J].,2019,(04):554.[doi:doi:10.3969/j.issn.1000-4440.2019.03.008]
[6]曹媛媛,陈春,郭婷婷,等.亲和性促生菌DW12-L的定殖及其对大豆生长的影响[J].江苏农业学报,2019,(04):776.[doi:doi:10.3969/j.issn.1000-4440.2019.04.004]
　CAO Yuan yuan,CHEN Chun,GUO Ting ting,et al.Colonization of soybean affinity rhizobacteria strain DW12-L and its effects on soybean growth[J].,2019,(04):776.[doi:doi:10.3969/j.issn.1000-4440.2019.04.004]
[7]丁俊男,于少鹏,李鑫,等.生物炭对大豆生理指标和农艺性状的影响[J].江苏农业学报,2019,(04):784.[doi:doi:10.3969/j.issn.1000-4440.2019.04.005]
　DING Jun nan,YU Shao peng,LI Xin,et al.Effects of biochar application on soybean physiological indices and agronomic traits[J].,2019,(04):784.[doi:doi:10.3969/j.issn.1000-4440.2019.04.005]
[8]曹帅,杜仲阳,刘鹏,等.碱胁迫对大豆光合特性及内源激素含量的影响[J].江苏农业学报,2020,(02):284.[doi:doi:10.3969/j.issn.1000-4440.2020.02.005]
　CAO Shuai,DU Zhong-yang,LIU Peng,et al.Effects of alkaline stress on photosynthetic characteristics and endogenous hormone contents of soybean[J].,2020,(04):284.[doi:doi:10.3969/j.issn.1000-4440.2020.02.005]
[9]邱爽,张军,何佳琦,等.大豆GmGolS2-1基因高温胁迫诱导表达及转基因烟草鉴定[J].江苏农业学报,2021,(01):38.[doi:doi:10.3969/j.issn.1000-4440.2021.01.005]
　QIU Shuang,ZHANG Jun,HE Jia-qi,et al.Expression of soybean GmGolS2-1 induced by heat stress and identification of GmGolS2-1 transgenic tobacco[J].,2021,(04):38.[doi:doi:10.3969/j.issn.1000-4440.2021.01.005]
[10]张斌,陈丽娟,李其华,等.栽培大豆GRAS转录因子家族基因鉴定及其盐胁迫下表达模式分析[J].江苏农业学报,2021,(02):296.[doi:doi:10.3969/j.issn.1000-4440.2021.02.004]
　ZHANG Bin,CHEN Li-juan,LI Qi-hua,et al.Identification of gene of GRAS transcription factor family in cultivated soybean（Glycine max L.） and expression pattern analysis under salt stress[J].,2021,(04):296.[doi:doi:10.3969/j.issn.1000-4440.2021.02.004]

备注/Memo

备注/Memo:
收稿日期：2024-10-09 基金项目：国家重点研发计划项目(2021YFD1400700)；粮食和蔬菜病虫害抗药性治理安徽省重点实验室开放课题项目(PKLPRMGVP202401)；重大林业有害生物安徽省重点实验室开放课题项目(AKL-RCEF-202404) 作者简介：胡飞(1981-),男,安徽合肥人,硕士,副研究员,主要从事农业害虫综合防治研究。(E-mail)hufly0224@163.com 通讯作者：徐丽娜,(E-mail)caasxln@163.com
更新日期/Last Update: 2025-05-26