[1]刘颖,陈甜甜,王玲,等.基于扬麦38/周麦22 RIL群体的株高QTL定位及矮秆多抗小麦材料的鉴定[J].江苏农业学报,2025,(04):635-643.[doi:doi:10.3969/j.issn.1000-4440.2025.04.002]
　LIU Ying,CHEN Tiantian,WANG Ling,et al.QTL mapping of plant height in Yangmai 38/Zhoumai 22 recombinant inbred line population and identificaiton of new dwarf wheat lines with multiple resistance[J].,2025,(04):635-643.[doi:doi:10.3969/j.issn.1000-4440.2025.04.002]
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基于扬麦38/周麦22 RIL群体的株高QTL定位及矮秆多抗小麦材料的鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年04期
页码:
635-643
栏目:
遗传育种·生理生化
出版日期:
2025-04-30

文章信息/Info

Title:
QTL mapping of plant height in Yangmai 38/Zhoumai 22 recombinant inbred line population and identificaiton of new dwarf wheat lines with multiple resistance
作者:
刘颖1; 2; 陈甜甜2; 王玲2; 陈树林3; 赵霈泽2; 4; 李俊梅2; 5; 梁倩1; 万文涛2; 别同德2; 赵仁慧2; 刘炳亮1
(1.扬州大学/江苏省粮食作物现代产业技术协同创新中心,江苏扬州225009；2.江苏里下河地区农业科学研究所/农业农村部长江中下游小麦生物学与遗传育种重点实验室,江苏扬州225007；3.河南农业大学农学院,河南郑州450002；4.云南农业大学农业与生物技术学院,云南昆明650000；5.长江大学农学院,湖北荆州434025)
Author(s):
LIU Ying1; 2; CHEN Tiantian2; WANG Ling2; CHEN Shulin3; ZHAO Peize2; 4; LI Junmei2; 5; LIANG Qian1; WAN Wentao2; BIE Tongde2; ZHAO Renhui2; LIU Bingliang1
(1.Yangzhou University/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou 225009, China;2.Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province/Key Laboratory of Wheat Biology and Genetic Breeding in the Middle and Lower Yangtze River, Ministry of Agriculture and Rural Affairs, Yangzhou 225007, China;3.College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China;4.College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650000, China;5.College of Agriculture, Yangtze University, Jingzhou 434025, China)
关键词:
小麦; 株高; 抗病基因; 分子标记
Keywords:
wheat; plant height; resistance genes; molecular markers
分类号:
S512.103.4
DOI:
doi:10.3969/j.issn.1000-4440.2025.04.002
文献标志码:
A
摘要:
长江中下游麦区是中国第二大麦区,也是小麦赤霉病、白粉病和锈病等病害的流行区。培育矮秆抗倒伏、综合抗病性强的小麦新品种是小麦绿色生产的保障。扬麦38是多抗高产小麦新品种,高抗白粉病(含Pm21基因)和条锈病(含Yr26基因),中抗-抗赤霉病,但其株高偏高,抗倒伏性一般。周麦22是黄淮麦区的矮秆多抗骨干亲本,携带抗叶锈病Lr13基因和T1RS·1BL易位染色体。本研究以扬麦38/周麦22重组自交系(Recombinant inbred lines, RIL)群体为试验材料,利用小麦55K SNP（单核苷酸多态性）芯片获取基因型,对株高进行数量性状座位(Quantitative trait loci, QTL)分析,共检测到6个QTL,分布于2B、4B、4D、5A和7B染色体上,其中QPH.yaas-4B、QPH.yaas-4D在多个环境中均可稳定检测到,分别可解释8.97%～16.20%、23.72%～28.35%的表型变异,通过物理图比对及竞争性等位基因特异性PCR（KASP）标记验证,确定它们分别为矮秆基因Rht1、Rht2。遗传效应分析结果表明,Rht1、Rht2均可显著降低小麦株高,Rht2对株高的降低效应大于Rht1,二者共存时可使株高降低42.98%～47.16%。RIL群体的抗病基因分布结果显示,Pm21、Lr13、Yr26基因和T1RS·1BL易位染色体的纯合基因型频率分别为40.77%、37.41%、63.07%和29.98%,均显著偏离了期望值(50.00%)。结合田间农艺评价,筛选出5份聚合Pm21、Yr26和Lr13基因的矮秆小麦育种新材料。研究结果可为长江中下游麦区小麦抗病聚合育种提供理论支撑。
Abstract:
The middle and lower reaches of the Yangtze River (MLRYR) is the second largest wheat-growing region in China and also an epidemic area for multiple wheat diseases, such as Fusarium head blight (FHB), powdery mildew, and rust. Cultivating new dwarf wheat varieties with resistance to lodging and multiple diseases is essential for sustainable wheat production. Yangmai 38 is a high-yielding wheat variety with multiple disease resistances, including high resistance to powdery mildew (carrying Pm21) and stripe rust (carrying Yr26), and moderate resistance to FHB. However, it has relatively tall plant height and moderate lodging resistance. Zhoumai 22 is a dwarf and multi-resistant foundation parent carrying the leaf rust resistance gene Lr13 and the T1RS·1BL translocation chromosome, and is widely used in the Huang-Huai wheat region. In this study, the Yangmai 38/Zhoumai 22 recombinant inbred line (RIL) population was genotyped using the wheat 55K single nucleotide polymorphism (SNP) array for quantitative trait loci (QTL) mapping of plant height (PH). Six QTL for PH were detected on chromosomes 2B, 4B, 4D, 5A, and 7B. Two major and stable QTL, QPH.yaas-4B and QPH.yaas-4D, were identified across multiple environments, explaining 8.97%-16.20% and 23.72%-28.35% of the phenotypic variance, respectively. These QTL were verified as dwarfing genes Rht1 and Rht2 through physical map comparison and kompetitive allele-specific PCR (KASP) markers. Genetic effect analysis showed that both Rht1 and Rht2 significantly reduced plant height, with Rht2 exhibiting a greater dwarfing effect than Rht1. The coexistence of Rht1 and Rht2 could reduce plant height by 42.98%-47.16%. The distribution of resistance genes in the RIL population was also investigated, with homozygous genotype frequencies for Pm21, Lr13, Yr26, and T1RS·1BL being 40.77%, 37.41%, 63.07%, and 29.98%, respectively, all of which significantly deviated from the expected value (50.00%). Based on genotyping and agronomic evaluation, five dwarf wheat breeding lines with pyramided Pm21, Yr26, and Lr13 were selected for their better comprehensive traits. The results of this study provide theoretical support for breeding wheat varieties with pyramided multi-disease resistance in the MLRYR wheat region.

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备注/Memo

备注/Memo:
收稿日期：2024-11-11基金项目：国家自然科学基金项目(32101737、32472102)；江苏里下河地区农业科学研究所科研专项[SJ(22)114]作者简介：刘颖(1999-),女,山东德州人,硕士研究生,主要从事小麦遗传育种研究。(E-mail)ly15628620431@163.com通讯作者：赵仁慧,(E-mail)zhaorh86@163.com;刘炳亮,(E-mail)blliu@yzu.edu.cn
更新日期/Last Update: 2025-05-26