[1]金昆,丛龙琦,穆东祺,等.干旱胁迫对冷蒿生长、生理指标和药效物质含量的影响[J].江苏农业学报,2025,(03):493-500.[doi:doi:10.3969/j.issn.1000-4440.2025.03.008]
　JIN Kun,CONG Longqi,MU Dongqi,et al.Impact of drought stress on the growth, physiological indices, and pharmacodynamic substance content of Artemisia frigida Willd.[J].,2025,(03):493-500.[doi:doi:10.3969/j.issn.1000-4440.2025.03.008]
点击复制

干旱胁迫对冷蒿生长、生理指标和药效物质含量的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年03期
页码:
493-500
栏目:
遗传育种·生理生化
出版日期:
2025-03-31

文章信息/Info

Title:
Impact of drought stress on the growth, physiological indices, and pharmacodynamic substance content of Artemisia frigida Willd.
作者:
金昆; 丛龙琦; 穆东祺; 曹阳; 张奥; 薛焱
(内蒙古医科大学药学院,内蒙古呼和浩特010110)
Author(s):
JIN Kun; CONG Longqi; MU Dongqi; CAO Yang; ZHANG Ao; XUE Yan
(School of Pharmacy, Inner Mongolia Medical University, Hohhot 010110, China)
关键词:
干旱胁迫; 冷蒿; 阿给; 生理指标; 药效物质
Keywords:
drought stress; Artemisia frigida Willd.; Agi; physiological parameters; pharmacodynamic substance
分类号:
R931.2
DOI:
doi:10.3969/j.issn.1000-4440.2025.03.008
文献标志码:
A
摘要:
本研究以冷蒿(Artemisia frigida Willd.)为试验材料,分别探究轻度干旱胁迫处理(土壤相对含水率保持在75%±5%)、中度干旱胁迫处理(土壤相对含水率保持在50%±5%)、重度干旱胁迫处理(土壤相对含水率保持在25%±5%)对冷蒿生长发育、抗氧化酶活性、活性氧含量、抗氧化物含量和药用物质含量的影响。结果表明,随干旱胁迫程度的提高,冷蒿叶片游离脯氨酸(Pro)含量、还原型抗坏血酸(AsA)含量、还原型谷胱甘肽(GSH)含量整体呈上升趋势,谷胱甘肽还原酶(GR)、过氧化氢酶(CAT)活性呈上升趋势。表明在受到干旱胁迫后,冷蒿能够维持动态平衡和相对稳定的内环境。随干旱胁迫程度的提高,冷蒿叶片叶周长、叶面积、叶长、叶宽、株高、茎粗等生长指标整体呈下降趋势,总黄酮含量呈上升趋势。相关性分析结果表明,冷蒿株高、茎粗等生长指标与干旱胁迫程度呈显著负相关(P＜0.05),总黄酮含量与干旱胁迫程度呈显著正相关(P＜0.05)。对冷蒿产量-品质线性回归模型进行分析,结果表明在含水率为57%～59%的土壤中栽培冷嵩可以较好地平衡其产量与质量。本研究结果可为蒙药药材冷蒿的优质高效栽培提供理论依据。
Abstract:
This study used Artemisia frigida Willd. as the experimental material to investigate the effects of mild drought stress (soil relative water content maintained at 75%±5%), moderate drought stress (soil relative water content maintained at 50%±5%), and severe drought stress (soil relative water content maintained at 25%±5%) on the growth and development of Artemisia frigida Willd., as well as its antioxidant enzyme activities, reactive oxygen species content, antioxidant substance content and pharmacodynamic substance content. The results showed that with the increase in drought stress intensity, the overall contents of free proline (Pro), reduced ascorbic acid (AsA), and reduced glutathione (GSH) in the leaves of Artemisia frigida Willd. increased, and the activities of glutathione reductase (GR) and catalase (CAT) also increased. The above results indicated that after being subjected to drought stress, Artemisia frigida Willd. could maintain dynamic equilibrium and a relatively stable internal environment. As the drought stress intensity increased, the growth indicators of Artemisia frigida Willd., such as leaf perimeter, leaf area, leaf length, leaf width, plant height, and stem diameter, showed an overall downward trend, while the total flavonoids content increased. The correlation analysis results showed that the growth indicators of Artemisia frigida Willd., such as plant height and stem diameter, were significantly negatively correlated with drought stress intensity (P＜0.05), while the total flavonoids content was significantly positively correlated with drought stress intensity (P＜0.05). The analysis results of the yield-quality linear regression model of Artemisia frigida Willd. indicated that cultivating it in soil with a water content of 57%-59% could achieve a better balance between yield and quality. This study provides a theoretical basis for the high-quality and efficient cultivation of the Mongolian medicinal herb Artemisia frigida Willd..

参考文献/References:

[1]薛焱,刘欣媛,高洪波,等. 蒙药阿给水提物的抗炎作用及其机制研究[J]. 中国药房,2020,31(12):1425-1429.
[2]王青虎,乌仁苏布德,布和巴特尔,等. 蒙药小白蒿的历史沿革与现代研究概述[J]. 中国现代应用药学,2010,27(10):897-900,915.
[3]陈越,宛涛,张晓明,等. 冷蒿资源利用研究进展[J]. 中国草地学报,2024,46(2):134-143.
[4]王昀,王丽娟,陆晓娟,等. 2023年上半年我国干旱的特征及其成因分析[J]. 干旱气象,2023,41(6):884-896.
[5]张一龙,喻启坤,李雯,等. 不同抗旱性狗牙根地上地下表型特征及内源激素对干旱胁迫的响应[J]. 草业学报,2023,32(3):163-178.
[6]孙娜,秦雪龙,梁锋娜,等. 不同干旱胁迫强度下的类芦生长权衡研究[J]. 草业科学,2023,40(6):1582-1591.
[7]刘付松,张开弦,姚秋阳,等. 干旱后复水对党参农艺性状、生理生化及代表组分的影响[J]. 中国药学杂志,2022,57(13):1081-1088.
[8]MACHADO J, FERNANDES A P G, BOKOR B, et al. The effect of silicon on the antioxidant system of tomato seedlings exposed to individual and combined nitrogen and water deficit[J]. Annals of Applied Biology,2024,184（1）:50-60.
[9]何凤,刘攀峰,王璐,等. 干旱胁迫及复水对杜仲苗生理特性的影响[J]. 植物生理学报,2021,57(3):661-671.
[10]张倩倩,李光跃,苏优拉,等. 干旱胁迫对蒙古黄芪和膜荚黄芪不同器官黄酮类成分积累的影响[J]. 西北植物学报,2020,40(7):1201-1208.
[11]杜虹韦,李孟,娄志红,等. 干旱胁迫下黄芩代谢变化揭示药材活性成分复杂性的生物学本质[J]. 中国实验方剂学杂志,2021,27(24):148-156.
[12]谢丰璞,王楠,高静,等. 干旱胁迫下药用大黄根部药效成分及根际土壤微生物变化规律及其相互作用机制研究[J]. 中国中药杂志,2023,48(6):1498-1509.
[13]KUNERT O, ALPERTH F, PABI E, et al. Highly oxidized flavones in Artemisia species - structure revisions and improved UHPLC-MSn analysis[J]. Heliyon,2023,9(11):e22309.
[14]FU Q H, MA X Q, LI S C, et al. New insights into the interactions between the gut microbiota and the inflammatory response to ulcerative colitis in a mouse model of dextran sodium sulfate and possible mechanisms of action for treatment with PE & AFWE[J]. Animal Models and Experimental Medicine,2024,7(2):83-97.
[15]牛雪婧,聂靖,杨自云,等. 河北木蓝叶表型对干旱胁迫的响应[J]. 西北植物学报,2020,40(4):613-623.
[16]布日额,阿拉腾图亚,包金泉,等. 蒙药材阿给的生药学研究[J]. 中国民族医药杂志,1998(增刊1):53.
[17]赵一之,曹瑞,赵利清. 内蒙古植物志 第5卷[M]. 3版. 呼和浩特:内蒙古人民出版社,2020.
[18]YUSUF M, SAEED T, ALMENHALI H A, et al. Melatonin improved efficiency of 24-epibrassinolide to counter the collective stress of drought and salt through osmoprotectant and antioxidant system in pea plants[J]. Scientia Horticulturae,2024,323:112453.
[19]赵晶晶,詹万龙,周浓. 非生物胁迫下植物体内活性氧和丙酮醛代谢的研究进展[J]. 南方农业学报,2022,53(8):2099-2113.
[20]CAO S S, SHI L, SHEN Y, et al. Ecological roles of secondary metabolites of Saposhnikovia divaricata in adaptation to drought stress[J]. PeerJ,2022,10:e14336.
[21]王青虎,武晓兰,王金辉. 蒙药小白蒿化学成分的研究(Ⅱ)[J]. 中草药,2011,42(6):1075-1078.
[22]武曦,张罡,郭华,等. 干旱胁迫对多裂叶荆芥幼苗形态和不同部位生理生化指标的影响[J]. 山西农业科学,2022,50(2):161-169,132.
[23]许彩丽. 遮阴与干旱胁迫对箭叶淫羊藿农艺及生理生化性状的影响研究[D]. 长沙:湖南农业大学,2021.
[24]CHEN Q, QU Z, MA G H, et al. Humic acid modulates growth,photosynthesis,hormone and osmolytes system of maize under drought conditions[J]. Agricultural Water Management,2022,263(4):107447.
[25]WANG Z H, WEI Y Q. Physiological and transcriptomic analysis of antioxidant mechanisms in sweet sorghum seedling leaves in response to single and combined drought and salinity stress[J]. Journal of Plant Interactions,2022,17(1):1006-1016.
[26]RAHMAT A, HASSANI H K, HOSSEIN M R. The simultaneous activity of cytosolic and mitochondrial antioxidant mechanisms in neutralizing the effect of drought stress in soybean[J]. Plant Physiology Reports,2022,28(1):78-91.
[27]郄亚微,贺涛. 褪黑素对模拟干旱胁迫下罗勒幼苗光合性能及抗氧化酶活性的影响[J]. 江苏农业科学,2024,52(3):179-184.
[28]李小玲,华智锐. 温度与水分胁迫下黄芩的渗透调节能力与交叉关系研究[J]. 江苏农业科学,2023,51(21):162-168.
[29]杨秋悦,罗影子,杨洋,等. 干旱胁迫对铁皮石斛生理及不同部位活性成分的影响[J]. 江苏农业科学,2023,51(13):142-149.
[30]JORGE T F, TOHGE T, WENDENBURG R, et al. Salt-stress secondary metabolite signatures involved in the ability of Casuarina glauca to mitigate oxidative stress[J]. Environmental and Experimental Botany,2019,166:103808.
[31]刘同歌,苑海鹏,叶云舒,等. 干旱胁迫和复水对火麻生理及次生代谢产物含量的影响[J]. 节水灌溉,2023,12:41-50.
[32]许丽丽,孟新亚,尤燕平,等. 血叶兰幼苗对干旱胁迫的生理响应及其抗旱性指标筛选[J]. 亚热带植物科学,2024,53(1):12-21.
[33]盛玉章. 水盐胁迫及施肥对甘草和秦艽品质的影响[D]. 咸阳:西北农林科技大学,2022.
[34]顾旭鹏,杨林林,董诚明,等. 中药材品质形成机制及调控研究进展[J]. 北方园艺,2024(6):129-137.

相似文献/References:

[1]佚名 佚名 佚名.三才期刊采编系统文章正在整理中…[J].江苏农业学报,2005,(01):5.
　XIE Xin,ZHAO Zhong.三才期刊采编系统文章正在整理中…[J].,2005,(03):5.
[2]佚名 佚名 佚名.三才期刊采编系统文章正在整理中…[J].江苏农业学报,2006,(01):5.
　XIE Xin,ZHAO Zhong.三才期刊采编系统文章正在整理中…[J].,2006,(03):5.
[3]熊洁,邹晓芬,邹小云,等.干旱胁迫对不同基因型油菜农艺性状和产量的影响[J].江苏农业学报,2015,(03):494.[doi:10.3969/j.issn.1000-4440.2015.03.005]
　XIONG Jie,ZOU Xiao-fen,ZOU Xiao-yun,et al.Effects of drought stress on agronomic traits and yield of different rapeseed genotypes[J].,2015,(03):494.[doi:10.3969/j.issn.1000-4440.2015.03.005]
[4]肇莹,杨镇,杨涛,等.植物内生菌醇提取物对草坪草抗旱性的影响[J].江苏农业学报,2015,(01):39.[doi:10.3969/j.issn.1000-4440.2015.01.006]
　ZHAO Ying,YANG Zhen,YANG Tao,et al.Influence of plant endophyte extract on turfgrass drought tolerance[J].,2015,(03):39.[doi:10.3969/j.issn.1000-4440.2015.01.006]
[5]孟力力,张俊,闻婧.干旱胁迫对彩叶草光合特性及叶片超微结构的影响[J].江苏农业学报,2015,(01):180.[doi:10.3969/j.issn.1000-4440.2015.01.028]
　MENG Li-li,ZHANG Jun,WEN Jing.Changes of photosynthetic characteristics of Coleus blumei and mesophyll cell ultrastructure in response to drought stress[J].,2015,(03):180.[doi:10.3969/j.issn.1000-4440.2015.01.028]
[6]张丽丽,徐碧玉,刘菊华,等.MaASR1基因通过乙烯途径提高拟南芥抗旱性的作用机制[J].江苏农业学报,2018,(03):511.[doi:doi:10.3969/j.issn.1000-4440.2018.03.005]
　ZHANG Li-li,XU Bi-yu,LIU Ju-hua,et al.The regulation mechanism of MaASR1 gene for improving the drought resistance of Arabidopsis by ethylene pathway[J].,2018,(03):511.[doi:doi:10.3969/j.issn.1000-4440.2018.03.005]
[7]麻云霞,李钢铁,张宏武,等.外源硅对酸枣生长和生理生化特征的影响[J].江苏农业学报,2018,(05):1113.[doi:doi:10.3969/j.issn.1000-4440.2018.05.021]
　MA Yun-xia,LI Gang-tie,ZHANG Hong-wu,et al.Effects of exogenous silicon on growth， physiological and biochemical characteristics of zizyphus jujube plant[J].,2018,(03):1113.[doi:doi:10.3969/j.issn.1000-4440.2018.05.021]
[8]葛道阔,曹宏鑫,杨余旺,等.干旱胁迫下油菜栽培模拟优化决策系统（Rape-CSODS）的订正及其检验[J].江苏农业学报,2019,(01):56.[doi:doi:10.3969/j.issn.1000-4440.2019.01.008]
　GE Dao-kuo,CAO Hong-xin,YANG Yu-wang,et al.Modification and verification of Rape-CSODS under drought stress[J].,2019,(03):56.[doi:doi:10.3969/j.issn.1000-4440.2019.01.008]
[9]陈丽,焦健,朱绍丹,等.油橄榄对牧草间作与干旱胁迫交互作用的根系生理响应[J].江苏农业学报,2019,(06):1434.[doi:doi:10.3969/j.issn.1000-4440.2019.06.024]
　CHEN Li,JIAO Jian,ZHU Shao-dan,et al.Root physiological response of olive to the interaction of pasture intercropping and drought stress[J].,2019,(03):1434.[doi:doi:10.3969/j.issn.1000-4440.2019.06.024]
[10]陈丽,焦健,朱绍丹,等.牧草间作对干旱胁迫下油橄榄根系形态特征的影响[J].江苏农业学报,2020,(01):39.[doi:doi:10.3969/j.issn.1000-4440.2020.01.006]
　CHEN Li,JIAO Jian,ZHU Shao-dan,et al.Effects of pasture intercropping on root morphological characteristics of olive under drought stress[J].,2020,(03):39.[doi:doi:10.3969/j.issn.1000-4440.2020.01.006]

备注/Memo

备注/Memo:
收稿日期：2024-07-09基金项目：内蒙古自然科学基金面上项目(2022MS08014)；内蒙古高等学校科学研究重点项目(NJZZ22647)；内蒙古医科大学面上项目(YKD2023MS083)作者简介：金昆(1999-),男,山东日照人,硕士研究生,研究方向为中蒙药资源开发。(E-mail)1540848500@qq.com通讯作者：薛焱,(E-mail)xyxy2172@163.com
更新日期/Last Update: 2025-04-27