[1]王楠艺,范高领,付文婷,等.辣椒C2H2型锌指蛋白家族基因鉴定及其在涝胁迫下的表达分析[J].江苏农业学报,2025,(01):134-149.[doi:doi:10.3969/j.issn.1000-4440.2025.01.016]
　WANG Nanyi,FAN Gaoling,FU Wenting,et al.Identification of the C2H2-type zinc finger protein family genes in Capsicum annuum L. and their expression analysis under waterlogging stress[J].,2025,(01):134-149.[doi:doi:10.3969/j.issn.1000-4440.2025.01.016]
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辣椒C2H2型锌指蛋白家族基因鉴定及其在涝胁迫下的表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年01期
页码:
134-149
栏目:
园艺
出版日期:
2025-01-31

文章信息/Info

Title:
Identification of the C2H2-type zinc finger protein family genes in Capsicum annuum L. and their expression analysis under waterlogging stress
作者:
王楠艺1; 范高领1; 付文婷1; 吴迪1; 杨娅1; 黄冬福1; 王立浩2; 吴康云3; 何建文1
(1.贵州省农业科学院辣椒研究所,贵州贵阳550006；2.中国农业科学院蔬菜花卉研究所,北京100081；3.农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵州贵阳550006)
Author(s):
WANG Nanyi1; FAN Gaoling1; FU Wenting1; WU Di1; YANG Ya1; HUANG Dongfu1; WANG Lihao2; WU Kangyun3; HE Jianwen1
(1.Institute of Pepper, Guizhou Academy of Agricultural Sciences, Guiyang 550006, China;2.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;3.Key Laboratory of Crop Genetic Resources and Germplasm Innovation in Karst Region, Ministry of Agriculture and Rural Affairs, Guiyang 550006, China)
关键词:
辣椒; C2H2型锌指蛋白; 涝胁迫; 生物信息学
Keywords:
Capsicum annuum L.; C2H2-type zinc finger protein; waterlogging stress; bioinformatics
分类号:
S641.3
DOI:
doi:10.3969/j.issn.1000-4440.2025.01.016
文献标志码:
A
摘要:
为了探究辣椒C2H2型锌指蛋白家族在响应涝胁迫中的作用,本研究通过生物信息学方法对辣椒C2H2型锌指蛋白家族蛋白理化性质、基因结构、染色体定位、顺式作用元件进行了全面分析。结果表明,辣椒C2H2型锌指蛋白家族包含91个成员,多数成员定位于细胞核。91个成员可被分为6个亚族,不同亚族的成员基因结构存在差异。C2H2型锌指蛋白家族基因启动子区域主要包含植物生长发育元件、植物激素响应元件及逆境胁迫响应元件。对耐涝辣椒资源GC41和涝胁迫敏感型辣椒资源GC39在涝胁迫下的转录组数据进行分析，结果表明，在GC41中,CaC2H2-17、CaC2H2-87在涝胁迫处理3 d时相对表达量高,CaC2H2-17、CaC2H2-87在GC39中未表达。筛选出CaC2H2-16(Capana01g003556)、CaC2H2-32(Capana04g001616)、CaC2H2-72(Capana11g000176)、 CaC2H2-73(Capana11g000178)、CaC2H2-87(Capana12g000773)5个转录因子,这5个转录因子分别调控了419个、526个、566个、305个、480个差异表达基因。本研究结果为辣椒C2H2家族成员的功能解析及耐涝辣椒资源的分子育种提供了重要的理论依据和数据支持。
Abstract:
To investigate the role of the Capsicum annuum L. C2H2-type zinc finger protein family in response to waterlogging stress, the physical and chemical properties, gene structure, chromosome localization, and cis-acting elements of the C2H2-type zinc finger protein family were comprehensively analyzed by bioinformatics methods. The results showed that the Capsicum annuum L. C2H2-type zinc finger protein family contained 91 members, most of which were located in the nucleus. The 91 members could be divided into six subfamilies, and the gene structure of members in different subamilies was different. The promoter regions of C2H2-type zinc finger protein family genes mainly included plant growth and development elements, plant hormone response elements, and abiotic stress response elements. The transcriptome data of the waterlogging-tolerant pepper GC41 and the waterlogging-sensitive pepper GC39 under waterlogging stress were analyzed. In GC41, CaC2H2-17 and CaC2H2-87 were highly expressed at 3 d under waterlogging stress, while CaC2H2-17 and CaC2H2-87 were not expressed in GC39. In this study, five transcription factors CaC2H2-16 (Capana01g003556), CaC2H2-32 (Capana04g001616), CaC2H2-72 (Capana11g000176), CaC2H2-73 (Capana11g000178), and CaC2H2-87 (Capana12g000773) were screened out, and these five transcription factors regulated 419, 526, 566, 305, and 480 differentially expressed genes, respectively. The results of this study provide important theoretical basis and data support for the functional analysis of C2H2 family members in Capsicum annuum L. and molecular breeding of waterlogging-tolerant pepper.

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备注/Memo

备注/Memo:
收稿日期：2024-06-03基金项目：贵州省农业科学院一般基金项目[黔农科一般基金(2024)26号]；贵州省山地农业关键核心技术攻关项目(GZNYGJHX-2023008)；贵州省辣椒产业技术体系遗传育种功能实验室项目(GZLJCYJSTX-02)作者简介：王楠艺(1997-),男,贵州毕节人,硕士,助理研究员,主要从事辣椒种质资源遗传改良与创新研究。(E-mail)wangnanyi0202@163.com通讯作者：何建文,(E-mail)569809092@qq.com
更新日期/Last Update: 2025-02-28