[1]万文涛,赵霈泽,陈甜甜,等.含抗白粉病基因PmV小麦-簇毛麦补偿性插入易位系Dv6-IT2的创制与鉴定[J].江苏农业学报,2024,(11):2013-2020.[doi:doi:10.3969/j.issn.1000-4440.2024.11.004]
　WAN Wentao,ZHAO Peize,CHEN Tiantian,et al.Creation and identification of compensating intercalary translocation line Dv6-IT2 carrying the powdery mildew resistance gene PmV from Dasyprum villosum[J].,2024,(11):2013-2020.[doi:doi:10.3969/j.issn.1000-4440.2024.11.004]
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含抗白粉病基因PmV小麦-簇毛麦补偿性插入易位系Dv6-IT2的创制与鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年11期
页码:
2013-2020
栏目:
植物保护
出版日期:
2024-11-30

文章信息/Info

Title:
Creation and identification of compensating intercalary translocation line Dv6-IT2 carrying the powdery mildew resistance gene PmV from Dasyprum villosum
作者:
万文涛1; 赵霈泽1; 2; 陈甜甜1; 王玲1; 汪尊杰1; 张旭3; 陈一鸣3; 别同德1; 赵仁慧1
(1.农业农村部长江中下游小麦生物学与遗传育种重点实验室/江苏里下河地区农业科学研究所,江苏扬州225007；2.云南农业大学农学与生物技术学院,云南昆明650000；3.南京农业大学作物遗传与种质创新利用国家重点实验室,江苏南京210095)
Author(s):
WAN Wentao1; ZHAO Peize1; 2; CHEN Tiantian1; WANG Ling1; WANG Zunjie1; ZHANG Xu3; CHEN Yiming3; BIE Tongde1; ZHAO Renhui1
(1.Key Laboratory of Wheat Biology and Genetic Improvement for Low Middle Yangtze Valley, Ministry of Agriculture and Rural Affairs/Institute of Agricultural Sciences of the Lixiahe District in Jiangsu Province, Yangzhou 225007, China;2.College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650000, China;3.State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
小麦; 簇毛麦; 抗白粉病基因PmV; 插入易位
Keywords:
wheat; Dasyprum villosum; powdery mildew resistance gene PmV; intercalary translocation
分类号:
S512.1
DOI:
doi:10.3969/j.issn.1000-4440.2024.11.004
文献标志码:
A
摘要:
白粉病是中国小麦主要病害之一。抗白粉病基因PmV和Pm21是来自不同簇毛麦种质的同源基因,分别以T6V#4S·6DL和T6V#2S·6AL易位系的形式被育种利用。Pm21基因在中国已得到广泛应用,而作为其后备抗源的PmV基因却极少被利用,主要原因是T6V#4S·6DL易位染色体的外源片段过大,在亲子代传递率低,育种利用困难。创制含PmV的补偿性小片段易位是提高传递率、减少外源基因冗余的重要途径。本研究利用均携带PmV基因的顶端易位系Dv6T25与近着丝粒易位系Dv6T36杂交,根据外源重叠区重组原理,在F2代分离群体中筛选出1个携PmV基因的补偿性中间插入易位系Dv6-IT2。利用簇毛麦参考基因组序列信息进行标记加密分析,结果表明易位片段双侧断裂重接位点分别介于35.1～36.3 Mb和85.1～85.3 Mb,外源易位片段长度约为50 Mb。将Dv6-IT2与大面积推广品种扬麦23杂交构建F2分离群体,标记结果显示,抗感比符合3∶1分离比,说明PmV基因在新型易位中可正常传递。本研究成功创制了Dv6-IT2,将促进PmV基因在小麦抗白粉病育种中的利用。
Abstract:
Powdery mildew is one of the main diseases of wheat in China. The powdery mildew resistance genes PmV and Pm21 are homologous genes from different Dasyprum villosum germplasm resources, which are used in breeding in the form of T6V#4S·6DL and T6V#2S·6AL translocation lines, respectively. Pm21 gene has been widely used in China, while PmV gene is rarely used. The main reason is that the exogenous fragment of T6V#4S·6DL translocation chromosome is too large, the transmission rate in the parent-offspring generation is low, and it is difficult to use in breeding. The creation of compensating small fragment translocation containing PmV is an important way to improve the transmission rate and reduce the redundancy of exogenous genes. In this study, we crossed the distal translocation line Dv6T25 carrying PmV with the proximal translocation line Dv6T36 carrying PmV. According to the principle of exogenous overlap region recombination, a compensatory intercalary translocation line Dv6-IT2 carrying PmV was screened in the F2 segregating population. Marker encryption analysis was performed using the reference genome sequence information of D. villosum. The results showed that the double-sided breakpoints of the translocation fragments were 35.1-36.3 Mb and 85.1-85.3 Mb, respectively, and the length of the exogenous translocation fragment was about 50 Mb. The F2 segregating population was constructed by crossing Dv6-IT2 with Yangmai 23. The marker results indicated that the ratio of resistance to susceptibility was consistent with the segregation ratio of 3∶1, indicating that the PmV gene could be transmitted normally in the new translocation. In this study, Dv6-IT2 was successfully created, which promoted the utilization of PmV gene in wheat powdery mildew resistance breeding.

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备注/Memo

备注/Memo:
收稿日期：2024-03-13 基金项目：国家自然科学基金项目(32072065、32201808)；江苏省重点研发计划项目(BE2022346)；扬州市“绿扬金凤计划”优秀博士项目作者简介：万文涛 (1991-),男,江苏连云港人,博士,助理研究员,主要从事小麦遗传育种研究。(E-mail)wentaowan2021@126.com 通讯作者：赵仁慧,(E-mail)zhaorh86@163.com
更新日期/Last Update: 2025-01-20