[1]赵小慧,刘冲,郁凯,等.利用小RNA深度测序技术鉴定江苏盐城辣椒病毒种类[J].江苏农业学报,2023,(01):37-43.[doi:doi:10.3969/j.issn.1000-4440.2023.01.005]
　ZHAO Xiao-hui,LIU Chong,YU Kai,et al.Identification of viruses from peppers in Yancheng, Jiangsu province by small RNA deep sequencing[J].,2023,(01):37-43.[doi:doi:10.3969/j.issn.1000-4440.2023.01.005]
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利用小RNA深度测序技术鉴定江苏盐城辣椒病毒种类()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2023年01期
页码:
37-43
栏目:
植物保护
出版日期:
2023-02-28

文章信息/Info

Title:
Identification of viruses from peppers in Yancheng, Jiangsu province by small RNA deep sequencing
作者:
赵小慧1; 刘冲1; 郁凯1; 钟明娟2; 郑佳秋1; 邢锦城1
（1.江苏沿海地区农业科学研究所,江苏盐城224002;2.诸城市农业农村局, 山东诸城262200）
Author(s):
ZHAO Xiao-hui1; LIU Chong1; YU Kai1; ZHONG Ming-juan2; ZHENG Jia-qiu1; XING Jin-cheng1
(1.Institute of Agricultural Sciences in the Coastal District of Jiangsu Province, Yancheng 224002, China;2.Zhucheng Agricultural and Rural Bureau, Zhucheng 262200, China)
关键词:
辣椒; 小RNA深度测序; 病毒病
Keywords:
pepper; small RNA deep sequencing; viral disease
分类号:
S436.418.1+2
DOI:
doi:10.3969/j.issn.1000-4440.2023.01.005
文献标志码:
A
摘要:
2019年6月江苏省盐城市发生了较为严重的辣椒病毒病，危害症状表现为植株重度矮化、叶片黄化、蕨叶甚至畸形。将采集的11株疑似感染病毒的辣椒植株叶片研磨成浆液，再用其摩擦接种本氏烟植株，发现用其中3株辣椒的叶片浆液摩擦接种本氏烟植株后，本氏烟植株出现病毒侵染的典型症状，初步判断这些辣椒被病毒感染，但不能确定其种类和归属。为了进一步明确辣椒感染的病毒类型，将3株辣椒样品混成2份样品利用小RNA深度测序技术和生物信息学分析法进行检测，结果发现样品1被蚕豆萎蔫病毒2号（Broad bean wilt virus 2，BBWV2）、苜蓿花叶病毒（Alfalfa mosaic virus, AMV）和辣椒脉斑驳病毒（Chilli veinal mottle virus, ChiVMV）3种病毒复合侵染，从样品2中检测到BBWV2 1种病毒，通过RT-PCR检测验证了这一结果的可靠性。最后利用RT-PCR方法对田间采集的11份辣椒样品和接种的本氏烟植株进行毒源鉴定，其中2株辣椒样品及其接种的本氏烟叶片上鉴定出了BBWV2、ChiVMV和AMV，这3种病毒侵染辣椒在江苏省均为首次发现。研究结果为对江苏省辣椒构成潜在严重威胁的病毒的早期诊断和及时防控提供了参考。
Abstract:
In June 2019, an incidence of relatively serious pepper virus disease was reported in Yancheng City, Jiangsu province. The symptoms included plant dwarf stature, yellowing and deformities of pepper leaves. Eleven strains collected from pepper leaves infected with suspected virus were inoculated into Nicotiana benthamiana leaves by surface rubbing and it was found that three strains of peppers had typical symptoms of virus infection after surface inoculation of N. benthamiana. It was preliminarily suggested that these peppers were infected by a virus with unknown identity. In order to further clarify the virus type, two samples obtained by mixing three pepper standard samples were detected by small RNA deep sequencing technology and bioinformatics analysis. It was found that sample 1 was co-infected by broad bean wilt virus 2 (BBWV2), alfalfa mosaic virus (AMV) and chilli veinal mottle virus (ChiVMV). One virus of BBWV2 was detected in sample 2, and RT-PCR detection verified the reliability of the results. Finally, 11 pepper samples collected from the field and inoculated N. benthamiana were identified by RT-PCR. This is the first report of BBWV2, ChiVMV and AMV viruses infecting peppers in Jiangsu. The current study acts as a base line for early diagnosis and timely prevention and control of novel viral strains that pose a potential threat to peppers in Jiangsu province.

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备注/Memo

备注/Memo:
收稿日期：2022-06-24基金项目：国家自然科学基金项目（31901856）；江苏省农业重大新品种创制项目（PZCZ201715)作者简介：赵小慧（1989-），女，山东莱阳人，博士，助理研究员，主要从事植物病毒的检验和检疫研究。（E-mail）zhaoxiaohui_g@163.com通讯作者：邢锦城，（E-mail）sdauxxx@163.com
更新日期/Last Update: 2023-03-21