[1]朱璐,闻婧,马秋月,等.鸡爪槭金陵丹枫和金陵黄枫叶片呈色分析[J].江苏农业学报,2022,38(02):521-527.[doi:doi:10.3969/j.issn.1000-4440.2022.02.028]
 ZHU Lu,WEN Jing,MA Qiu-yue,et al.Analysis on leaf colour generation of Acer palmatum Jinling Danfeng and Jinling Huangfeng[J].,2022,38(02):521-527.[doi:doi:10.3969/j.issn.1000-4440.2022.02.028]
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鸡爪槭金陵丹枫和金陵黄枫叶片呈色分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
38
期数:
2022年02期
页码:
521-527
栏目:
园艺
出版日期:
2022-04-30

文章信息/Info

Title:
Analysis on leaf colour generation of Acer palmatum Jinling Danfeng and Jinling Huangfeng
作者:
朱璐闻婧马秋月颜坤元杜一鸣李淑顺李倩中
(江苏省农业科学院休闲农业研究所,江苏南京210014)
Author(s):
ZHU LuWEN JingMA Qiu-yueYAN Kun-yuanDU Yi-mingLI Shu-shunLI Qian-zhong
(Institute of Leisure Agriculture,Jiangsu Academy of Agricultural Sciences, Nanjing 210014,China)
关键词:
鸡爪槭叶片色素
Keywords:
Acer palmatumleafpigment
分类号:
S687
DOI:
doi:10.3969/j.issn.1000-4440.2022.02.028
文献标志码:
A
摘要:
以江苏省农业科学院自主选育的鸡爪槭新品种金陵丹枫和金陵黄枫为研究对象,对其4月至7月观赏期叶片进行叶色对比观察,发现金陵丹枫新叶为亮红色,而后红色逐渐变淡,而金陵黄枫新叶为黄色,边缘呈浅珊瑚色,而后逐渐变为金黄色。比对金陵丹枫和金陵黄枫叶片中的叶绿素、类胡萝卜素、花青素和黄酮类物质含量,发现金陵丹枫亮红色叶片中各种色素含量均高于同时期的金陵黄枫金黄色叶片,且花青素和黄酮生物合成途径中一系列关键基因(ApPAL、ApCHS、ApCHI、ApF3H、ApF3′H、ApDFR和ApANS)的相对表达量在金陵丹枫叶片中上调。研究结果提示,这些色素含量的不同可能是导致金陵丹枫和金陵黄枫叶片呈色不同的主要原因。尤其是3种花青素(飞燕草素、矢车菊素和矢车菊半乳糖苷)在金陵丹枫亮红色叶片中的含量远高于金陵黄枫金黄色叶片,表明花青素在金陵丹枫叶片呈色中起到关键作用。
Abstract:
New varieties of Acer palmatum Thunb. named Jinling Danfeng and Jinling Huangfeng breeded by Jiangsu Academy of Agricultural Sciences independently were used as experimental materials and colours of the leaves during ornamental period from April to July were observed and compared. It was found that the new leaves of Jinling Danfeng were bright red and then the colour gradually faded, while the new leaves of Jinling Huangfeng were yellow with light coral on the edges, which gradually turned into golden yellow. After comparing the contents of chlorophyll, carotenoids, anthocyanins and flavonoids in the leaves of Jinling Danfeng and Jinling Huangfeng, it was found that contents of various pigments in the bright red leaves of Jinling Danfeng were higher than that in golden yellow leaves of Jinling Huangfeng at the same period, and the relative transcript levels of a series of key genes (ApPAL, ApCHS, ApCHI, ApF3H, ApF3′H, ApDFR and ApANS) in anthocyanin and flavonoid biosynthetic pathways in the leaves of Jinling Danfeng were up-regulated. The results indicated that, differences in pigments content may be the main reason for the different colours generation in Jinling Danfeng and Jinling Huangfeng leaves. In particular, the contents of three anthocyanins (delphinidin, cyanidin and cyanidin 3-galactoside) in the bright red leaves of Jinling Danfeng were much higher than those in the golden yellow leaves of Jinling Huangfeng, indicating that the anthocyanins played a key role in the colour generation in the leaves of Jinling Danfeng.

参考文献/References:

[1]LI S S, LI Q Z, Rong L P, et al. Gene expressing and sRNA sequencing show that gene differentiation associates with a yellow Acer palmatum mutant leaf in different light conditions[J]. Biomed Research International, 2015, 2015:1-10.
[2]KIM J H, LEE B C, SIM G S, et al. The isolation and antioxidative effects of vitexin from Acer palmatum [J]. Archives of Pharmacal Research, 2005,28:195.
[3]徐明远,何鹏,赖伟,等. 植物叶色变异分子机制研究进展[J].分子植物育种, 2021, 19(10):3448-3455.
[4]TANAKA Y, SASAKI N, OHMIYA A. Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids[J]. The Plant Journal, 2008,54:733-749.
[5]ZHAO D Q, TAO J. Recent advances on the development and regulation of flower color in ornamental plants[J]. Frontiers in Plant Science, 2015,6:261.
[6]KRAUTLER B. Breakdown of chlorophyll in higher plants-phyllobilins as abundant, yet hardly visible signs of ripening, senescence, and cell death[J]. Angewandte Chemim-International Edition, 2016, 55(16):4882-4907.
[7]SCHELBERT S, AUBYR S, BURTA B, et al. Pheophytin pheophorbide hydrolase (pheophytinase) is involved in chlorophyll breakdown during leaf senescence in Arabidopsis[J]. Plant Cell, 2009, 21(3):767-785.
[8]ZHANG T, FENG P, LI Y F, et al. VIRESCENT-ALBINO LEAF 1 regulates leaf colour development and cell division in rice[J]. Journal of Experimental Botany, 2018, 69(20): 4791-4804.
[9]ZHENG H,WANG Z R, TIAN Y L, et al. Rice albino 1, encoding a glycyl-tRNA synthetase, is involved in chloroplast development and establishment of the plastidic ribosome system in rice[J]. Plant Physiology and Biochemistry, 2019, 139:495-503.
[10]CAZZONELLI C I, POGSON B J. Source to sink: regulation of carotenoid biosynthesis in plants[J]. Trends in Plant Science, 2010, 15(5): 266-274.
[11]GADY A L F, VRIEZEN W H, VAN DE WAL M H B J, et al. Induced point mutations in the phytoene synthase 1 gene cause differences in carotenoid content during tomato fruit ripening[J]. Molecular Breeding, 2012, 29(3): 801.
[12]LI W X, YANG S B, LU Z G, et al. Cytological, physiological, and transcriptomic analyses of golden leaf coloration in Ginkgo biloba L[J]. Horticulture Research, 2018, 5:12.
[13]刘恺媛,王茂良,辛海波,等. 植物花青素合成与调控研究进展[J]. 中国农学通报, 2021, 37(14):41-51.
[14]SMERIGLIO A, BARRECA D, BELLOCCO E. et al. Chemistry, pharmacology and health benefits of anthocyanins[J]. Phytotherapy Research, 2016, 30(8):1265-1286.
[15]ITOH Y, HIGETA D, SUZUKI A, et al. Excision of transposable elements from the chalcone isomerase and dihydroflavonol 4-reductase genes may contribute to the variegation of the yellow-flowered carnation (Dianthus caryophyllus)[J]. Plant and Cell Physiology, 2002, 43(5):578-585.
[16]NISHIHARA M, NAKATSUKA T, YAMAMURA S. Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene[J]. FEBS Letters, 2005, 579(27): 579.
[17]SUN W, MENG X Y, LIANG L J, et al. Molecular and biochemical analysis of chalcone synthase from freesia hybrid in flavonoid biosynthetic pathway[J]. Plos One, 2015, 10(3):e0119054.
[18]TAI D Q, TIAN J, ZHENG J, et al. A malus crabapple chalcone synthase gene, mcchs, regulates red petal color and flavonoid biosynthesis[J]. Plos One, 2014, 9(10): e110570.
[19]WANG H L, WANG W, LI H, et al. Expression and tissue and subcellular localization of anthocyanidin synthase (ANS) in grapevine[J]. Protoplasma, 2011, 248(2): 267.
[20]ZHU L, MA Q Y, LI S S, et al. Reference gene selection for quantitative real-time PCR analyses of Acer palmatum under abiotic stress[J]. Phyton-International Journal of Experimental Botany, 2020, 89(2):385-403.
[21]TANAKA Y, BRUGLIERA F, CHANDLER S. Recent progress of flower colour modification by biotechnology[J]. International Journal of Molecular Sciences, 2009, 10(12):5350-5369.
[22]CHEN Z, LU X Y, XUAN Y, et al. Transcriptome analysis based on a combination of sequencing platforms provides insights into leaf pigmentation in Acer rubrum[J]. BMC Plant Biology, 2019, 19(1):240.
[23]LI S S, LI Q Z, T L, et al. Pigment comparison and expression of chlorophyll metabolism genes in yellow and green Acer palmatum leaves[J]. Canadian Journal of Plant Scienc, 2017, 97(5), 1-21.
[24]李倩中,李淑顺,荣立苹,等. 鸡爪槭新品种‘金陵黄枫’[J]. 园艺学报, 2011, 38(8):1627-1628.
[25]闻婧,李淑顺,朱璐,等. 鸡爪槭新品种‘金陵丹枫’[J]. 园艺学报, 2019,46(S2):2920-2921.
[26]MASUDA T, FUJITA Y. Regulation and evolution of chlorophyll metabolism[J]. Photochemical & Photobiological Sciences Official Journal of the European Photochemistry Association & the European Society for Photobiology, 2008, 7(10):1131-1149.
[27]DEMMING A B, ADAMS W W. The role of xanthophyll cycle carotenoids in the protection of photosynthesis[J]. Trends in Plant Science, 1996, 1(1): 21-26.
[28]DONG X Y, HUANG L B, CHEN Q S, et al. Physiological and anatomical differences and differentially expressed genes reveal yellow leaf coloration in Shumard oak[J]. Plants, 2020, 9(2):169.
[29]JIANG Y, SONG H Y, HE J R, et al. Comparative transcriptome analysis provides global insight into gene expression differences between two orchid cultivars[J]. PLoS One, 2018,13(7):e0200155.
[30]JUN J H, XIAO X, RAO X, et al. Proanthocyanidin subunit composition determined by functionally diverged dioxygenases[J]. Nature Plants, 2018, 4(12):1034-1043.
[31]GUO J, HAN W, WANG M H. Ultraviolet and environmental stresses involved in the induction and regulation of anthocyanin biosynthesis: a review[J]. African Journal of Biotechnology, 2008, 25:1-4.
[32]练冬梅,赖正锋,姚运法,等.玫瑰茄转录组测序及花青素合成相关基因表达分析[J].江苏农业科学,2020,48(6):41-45.
[33]MANO H, OGASAWARA F, SATO K, et al. Isolation of a regulatory gene of anthocyanin biosynthesis in tuberous roots of purple-fleshed sweet potato[J]. Plant Physiology, 2007, 143(3):1252-1268.
[34]CHIOU C Y, YEH K W. Differential expression of MYB gene (OgMYB1) determines color patterning in floral tissue of Oncidium Gower Ramsey[J]. Plant Molecular Biology, 2008, 66(4):379-388.
[35]DAVIES K M, ALBERT N W, SCHWINN K E. From landing lights to mimicry: the molecular regulation of flower colouration and mechanisms for pigmentation patterning[J]. Functional Plant Biology, 2012, 39:619-638.
[36]RAMSAY N A, GLOVER B J. MYB-b HLH-WD40 protein complex and the evolution of cellular diversity[J]. Trends in Plant Science, 2005, 10:63-70.
[37]ZHU Z X, WANG H L, WANG Y Y, et al. Characterization of the cis elements in the proximal promoter regions of the anthocyanin pathway genes reveals a common regulatory logic that governs pathway regulation[J]. Journal of Experimental Botany, 2015, 66:3775-3789.

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备注/Memo

备注/Memo:
收稿日期:2021-08-19基金项目:国家自然科学基金项目(32001357);江苏省农业科技自主创新资金项目[CX(18)3073]作者简介:朱璐(1987-),女,山东淄博人,博士,副研究员,研究方向为鸡爪槭遗传育种。(E-mail)luzhu@jaas.ac.cn通讯作者:李倩中,(E-mail)qianzhongli@jaas.ac.cn;李淑顺,(E-mail)shushunli@jaas.ac.cn
更新日期/Last Update: 2022-05-07