[1]刘雪梅,王悦,李玮琦,等.辣椒miR169及其靶基因的生物信息学分析[J].江苏农业学报,2021,(06):1510-1515.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]
 LIU Xue-mei,WANG Yue,LI Wei-qi,et al.Bioinformatics analysis of miR169 and its target genes in pepper[J].,2021,(06):1510-1515.[doi:doi:10.3969/j.issn.1000-4440.2021.05.020]
点击复制

辣椒miR169及其靶基因的生物信息学分析()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年06期
页码:
1510-1515
栏目:
园艺
出版日期:
2021-12-30

文章信息/Info

Title:
Bioinformatics analysis of miR169 and its target genes in pepper
作者:
刘雪梅王悦李玮琦魏继承任如意郝爱平
(牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011)
Author(s):
LIU Xue-meiWANG YueLI Wei-qiWEI Ji-chengREN Ru-yiHAO Ai-ping
( College of Life Sciences and Technology, Mudanjiang Normal College, Mudanjiang 157011, China)
关键词:
miR169辣椒生物信息学NF-YA
Keywords:
miR169pepperbioinformaticsNF-YA
分类号:
S641.3
DOI:
doi:10.3969/j.issn.1000-4440.2021.05.020
文献标志码:
A
摘要:
为了解miR169基因家族在辣椒生长发育过程的重要调控作用,通过生物信息学的方法对can-miR169基因家族的成员进行了染色体定位、序列保守性分析、系统发育进化树分析、前体序列二级结构预测和靶基因预测。结果表明:can-miR169家族成员定位在4条染色体上;成熟序列高度保守,前体序列在产生成熟序列的位置高度保守;预测结果显示can-miR169家族成员均有靶基因,这些靶基因主要以靶基因核因子A(NF-YA)复合物为主,且大体相同。这些靶基因可以参与到种子萌发、植株发育和对外界环境胁迫的应答等多种生命活动中。
Abstract:
In order to understand the important regulatory role of miR169 gene family in the growth and development of pepper, the members of can-miR169 gene family were analyzed by bioinformatics, including chromosome mapping, sequence conservation analysis, phylogenetic tree analysis, precursor sequence secondary structure prediction and target gene prediction. The results showed that the members of can-miR169 family were located on four chromosomes, the mature sequence was highly conserved. The precursor sequence was highly conserved in the position where the mature sequence was produced. The forecasting results indicated that all members of can-miR169 family had target genes, which were mainly composed of nuclear factor Y (NF-YA) complexes and were roughly the same. These target genes can be involved in a variety of life activities such as seed germination, plant development and response to external environmental stress.

参考文献/References:

[1]ZHANG B, WANG Q. MicroRNA-based biotechnology for plant improvement[J]. J Cell Physiol, 2015,230(1):1-15.
[2]SZWEYKOWSKA-KULINSKA Z, JARMOLOWSKI A. Post-transcriptional regulation of microRNA accumulation and function: New insights from plants[J]. Mol Plant,2018,11(8):1006-1007.
[3]李尧尧,常怀成,周海霞,等. 黄瓜CsamiR156a及其靶基因CsSPLs的生物信息学分析[J].分子植物育种,2021,19(7):2169-2176.
[4]LI Y, ZHAO S L, LI J L, et al. Osa-miR169 negatively regulates rice immunity against the blast fungus magnaporthe oryzae[J].Front Plant Sci, 2017,8:2.
[5]陈禹彤,陈华民,余超,等. 水稻 miR169o及其靶基因 OsNF-YAs 对缺水胁迫的早期表达模式[J].生物技术通报,2015,31(8):76-81.
[6]舒李露,孙志超,张玮,等. 山核桃 miR169克隆及功能分析[J].核农学报,2017,31(8):1453-1462.
[7]阮先乐,王俊生,刘红占,等. 油菜miR169基因家族的生物信息学分析及靶基因预测[J].浙江农业学报,2018,30(8):1273-1280.
[8]董云,王毅,靳丰蔚,等. 油菜Bna-miR169基因的分离与过表达初步分析[J].西北农业学报,2016,25(12):1809-1815.
[9]方辉,曲俊杰,孙嘉曼,等. 葡萄miR169及其靶基因的生物信息学分析[J].南方农业学报,2017,48(8):1329-1334.
[10]SORIN C, DECLERCK M, CHRIST A,et al. A miR169 isoform regulates specific NF-YA targets and root architecture in Arabidopsis[J]. New Phytol, 2014,202(4):1197-1211.
[11]SERIVICHYASWAT P T, SUSILA H, AHN J H. Elongated hypocotyl 5-homolog (HYH) negatively regulates expression of the ambient temperature-responsive microRNA gene MIR169[J].Front Plant Sci,2017,8:2087.
[12]HANEMIAN M, BARLET X, SORIN C, et al. Arabidopsis CLAVATA1 and CLAVATA2 receptors contribute to Ralstonia solanacearum pathogenicity through a miR169-dependent pathway[J].New Phytol, 2016,211(2):502-515.
[13]YU Y, NI Z, WANG Y, et al. Overexpression of soybean miR169c confers increased drought stress sensitivity in transgenic Arabidopsis thaliana[J]. Plant Sci,2019,285:68-78.
[14]ZHANG X, ZOU Z, GONG P,et al. Over-expression of microRNA169 confers enhanced drought tolerance to tomato[J]. Biotechnol Lett,2011,33(2):403-409.
[15]MARCOANTONIO L G, ENRIQUE I L, ALFREDO C R,et al. Functional and transcriptome analysis reveals an acclimatization strategy for abiotic stress tolerance mediated by arabidopsis NF-YA family members[J],PLoS One, 2012,7(10):e48138.
[16]NI Z, HU Z, JIANG Q, et al. GmNFYA3, a target gene of miR169, is a positive regulator of plant tolerance to drought stress[J]. Plant Mol Biol, 2013,82(1/2):113-129.
[17]李小鹏,徐刚,郭世荣,等. 外源生长调节剂控制番茄和辣椒徒长及对产量和品质的影响[J]. 江苏农业科学,2020,48(5):126-131.
[18]潘宝贵,钱恒彦,戈伟,等.辣椒应答冷信号转导机制研究进展[J].江苏农业学报,2019,35(3):743-748.
[19]余小兰,张静,邹雨坤,等.不同施硒量对辣椒硒、磷、钾含量及产量的影响[J].江苏农业科学,2019,47(24):146-149.
[20]陈传亮,司素琴,张焕丽,等. 辣椒幼苗对干旱胁迫的生理响应[J].安徽农业科学,2018,46(14):60-61,63.
[21]LUAN M, XU M, LU Y, et al. Expression of zma-miR169 miRNAs and their target ZmNF-YA genes in response to abiotic stress in maize leaves[J]. Gene,2015,555(2):178-185.
[22]蒋梦琦,苏立遥,黄倏祺,等. 龙眼miR156家族及其调控靶标SPL的生物信息学和表达模式[J].应用与环境生物学报,2020,26(3):558-565.
[23]XING L, ZHU M, ZHANG M, et al. High-throughput sequencing of small RNA transcriptomes in maize kernel identifies miRNAs involved in embryo and endosperm development[J]. Genes (Basel),2017, 8(12):385.
[24]李崇奇,沈文涛,言普,等. 猴面花microRNA及靶基因的生物信息学预测[J].贵州农业科学,2015,43(1):8-12.
[25]徐妙云,朱佳旭,张敏,等. 植物miR169/NF-YA调控模块研究进展[J].遗传,2016,38(8):700-706.
[26]ZHAO B, GE L, LIANG R, et al. Members of miR-169 family are induced by high salinity and transiently inhibit the NF-YA transcription factor[J]. BMC Mol Biol,2009,10:29.
[27]LI W X, OONO Y, ZHU J, et al. The Arabidopsis NFYA5 transcription factor is regulated transcriptionally and posttranscriptionally to promote drought resistance[J].Plant Cell,2008,20(8):2238-2251.

相似文献/References:

[1]吕敏,苏建坤,白和盛,等.桃蚜取食和机械损伤对番茄和辣椒 PAL、LOX 和 PPO 活性的诱导作用[J].江苏农业学报,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
 Lv?Min,SU Jian-kun,BAI He-sheng,et al.The activities of PAL, LOX and PPO in tomato and pepper plants induced by aphid herbivory and mechanical damage[J].,2016,(06):1273.[doi:doi:10.3969/j.issn.1000-4440.2016.06.013]
[2]吴淑华,赵文浩,李廷芳,等.南京辣椒上一种斑驳类型病毒病的分子鉴定[J].江苏农业学报,2015,(06):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
 WU Shu-hua,ZHAO Wen-hao,LI Ting-fang,et al.Molecular identification of a virus causing mottle symptoms in pepper leaves in Nanjing[J].,2015,(06):1284.[doi:doi:10.3969/j.issn.1000-4440.2015.06.014]
[3]郭广君,孙茜,刘金兵,等.基于辣椒基因组重测序的InDel标记开发及应用[J].江苏农业学报,2015,(06):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
 GUO Guang-jun,SUN Qian,LIU Jin-bing,et al.Development and application of pepper InDel markers based on genome re-sequencing[J].,2015,(06):1400.[doi:doi:10.3969/j.issn.1000-4440.2015.06.032]
[4]李廷芳,吴淑华,赵文浩,等.青海海东设施辣椒轻斑驳病毒的分子检测[J].江苏农业学报,2017,(04):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
 LI Ting-fang,WU Shu-hua,ZHAO Wen-hao,et al.Molecular detection of mild mottle virus isolated from pepper in Haidong, Qinghai province[J].,2017,(06):958.[doi:doi:10.3969/j.issn.1000-4440.2017.04.036]
[5]刘潮,韩利红,宋培兵,等.辣椒类甜蛋白基因家族鉴定及表达分析[J].江苏农业学报,2018,(01):122.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
 LIU Chao,HAN Li-hong,SONG Pei-bing,et al.Identification and expression analysis of thaumatin-like protein gene in pepper[J].,2018,(06):122.[doi:doi:10.3969/j.issn.1000-4440.2018.01.018]
[6]王运儒,秦玉燕,杨秀娟,等.40%氯虫·噻虫嗪水分散粒剂在辣椒及土壤中的残留消解动态[J].江苏农业学报,2018,(01):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
 WANG Yun-ru,QIN Yu-yan,YANG Xiu-juan,et al.Dissipation of chlorantraniliprole and thiamethoxam in pepper and soil after field application in the form of 40% water dispersible granules[J].,2018,(06):207.[doi:doi:10.3969/j.issn.1000-4440.2018.01.030]
[7]潘宝贵,钱恒彦,戈伟,等.辣椒应答冷信号转导机制研究进展[J].江苏农业学报,2019,(03):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
 PAN Bao-gui,QIAN Heng-yan,GE Wei,et al.Research progress of cold signal transduction mechanisms in pepper[J].,2019,(06):743.[doi:doi:10.3969/j.issn.1000-4440.2019.03.034]
[8]高晶霞,吴雪梅,牛勇琴,等.辣根素水乳剂对连作辣椒生长及土壤酶活性的影响[J].江苏农业学报,2021,(01):116.[doi:doi:10.3969/j.issn.1000-4440.2021.01.015]
 GAO Jing-xia,WU Xue-mei,NIU Yong-qin,et al.Effect of athomin water emulsion on growth and soil enzyme activities of continuous cropping pepper[J].,2021,(06):116.[doi:doi:10.3969/j.issn.1000-4440.2021.01.015]
[9]郭广君,朱雪梅,潘宝贵,等.利用InDel分子标记辅助选育辣椒抗黄瓜花叶病毒病种质[J].江苏农业学报,2021,(05):1251.[doi:doi:10.3969/j.issn.1000-4440.2021.05.021]
 GUO Guang-jun,ZHU Xue-mei,PAN Bao-gui,et al.Innovation of pepper germplasm resource with resistance to cucumber mosaic virus by InDel molecular marker assisted selection[J].,2021,(06):1251.[doi:doi:10.3969/j.issn.1000-4440.2021.05.021]
[10]朱雪梅,郭广君,潘宝贵,等.辣椒抗PMMoV基因L4连锁标记的验证分析[J].江苏农业学报,2022,38(06):1627.[doi:doi:10.3969/j.issn.1000-4440.2022.06.022]
 ZHU Xue-mei,GUO Guang-jun,PAN Bao-gui,et al.Test analysis of L4-linked markers for PMMoV resistant gene in pepper[J].,2022,38(06):1627.[doi:doi:10.3969/j.issn.1000-4440.2022.06.022]

备注/Memo

备注/Memo:
收稿日期:2021-04-20基金项目:牡丹江师范学院科研项目(YB2020005);黑龙江省省属高等学校基本科研业务费科研项目(1353MSYYB011)作者简介:刘雪梅(1996-)女,贵州黔东南人,硕士,研究方向为分子生物学与生物化学。(E-mail)lxm12252021@163.com通讯作者:郝爱平,(E-mail)swxhap@126.com
更新日期/Last Update: 2022-01-07