[1]蒋薇,靳容,刘明,等.甘薯IbHKT-like基因的克隆与表达分析[J].江苏农业学报,2021,(04):831-838.[doi:doi:10.3969/j.issn.1000-4440.2021.04.003]
　JIANG Wei,JIN Rong,LIU Ming,et al.Cloning and expression analysis of IbHKT-like gene in sweet potato[J].,2021,(04):831-838.[doi:doi:10.3969/j.issn.1000-4440.2021.04.003]
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甘薯IbHKT-like基因的克隆与表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年04期
页码:
831-838
栏目:
遗传育种·生理生化
出版日期:
2021-08-28

文章信息/Info

Title:
Cloning and expression analysis of IbHKT-like gene in sweet potato
作者:
蒋薇1; 靳容1; 刘明1; 赵鹏1; 张爱君1; 王丹凤1; 李铁鑫2; 范文静2; 唐忠厚1
（1.江苏徐淮地区徐州农业科学研究所/中国农业科学院甘薯研究所，江苏徐州221131；2.安徽农业大学农学院，安徽合肥230036）
Author(s):
JIANG Wei1; JIN Rong1; LIU Ming1; ZHAO Peng1; ZHANG Ai-jun1; WANG Dan-feng1; LI Tie-xin2; FAN Wen-jing2; TANG Zhong-hou1
(1.Xuzhou Institute of Agricultural Sciences of Xuhuai District of Jiangsu Province/Sweet Potato Research Institute，Chinese Academy of Agricultural Sciences，Xuzhou 221131，China；2.College of Agronomy，Anhui Agricultural University，Hefei 230036，China)
关键词:
甘薯; IbHKT-like基因; 基因克隆; 表达分析; 亚细胞定位
Keywords:
sweet potato; IbHKT-like gene; gene cloning; expression analysis; subcellular localization
分类号:
Q786
DOI:
doi:10.3969/j.issn.1000-4440.2021.04.003
文献标志码:
A
摘要:
植物高亲和钾转运体HKT基因具有Na+（或K+）单向运输或Na+-K+共转运作用。为了探究甘薯高亲和钾转运体HKT的离子转运情况及其对非生物胁迫的响应，本研究克隆得到1个甘薯钾离子转运体IbHKT-like 基因。生物信息学分析结果表明，IbHKT-like基因序列全长为1 647 bp，编码548个氨基酸。IbHKT-like蛋白有2个TrkH（细菌钾转运系统Trk亚基）保守结构域，10个跨膜片段。进化树分析结果表明，IbHKT-like蛋白与旋花科的矮牵牛InHKT6氨基酸序列十分相似，相似度为90.63%。亚细胞定位结果显示，IbHKT-like蛋白主要定位在细胞质膜，在叶绿体中存在少量分布。组织特异性分析结果表明，IbHKT-like基因在叶中表达量最高。实时荧光定量PCR结果显示，IbHKT-like基因的表达受到低温、干旱、高盐及过氧化氢胁迫的诱导，说明IbHKT-like基因可能在甘薯抵御非生物胁迫中发挥着重要的作用。
Abstract:
The plant high affinity potassium transporter HKT gene had Na+ (or K+) unidirectional transport effect or Na+-K+co-transport effect. To explore the ion transport of high affinity potassium transporter HKT in sweet potato and its response to abiotic stress, a potassium transporter IbHKT-like gene of sweet potato was cloned in this study. Results of bioinformatics analysis showed that, the full length of IbHKT-like gene sequence was 1 647 bp, encoding 548 amino acids. The IbHKT-like protein had two TrkH (Trk subunit of potassium transport system in bacteria) conservative domains and ten transmembrane fragments. Results of evolutionary tree analysis showed that, the amino acid sequence of IbHKT-like protein was very similar to that of InHKT6 in petunia of Convolvulaceae, with the similarity of 90.63%. Subcellular localization showed that, the IbHKT-like protein mainly located in the cytoplasmic membrane and rarely located in the chloroplast. Results of tissue-specific analysis showed that, the expression quantity of IbHKT-like gene was the highest in the leaves. The results of real-time fluorescent quantitative PCR showed that, the expression of IbHKT-like gene was induced by low temperature, drought, high salinity and hydrogen peroxide, indicating that IbHKT-like gene may play an important role in the resistance of sweet potato to abiotic stress.

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备注/Memo

备注/Memo:
收稿日期：2020-12-06基金项目：国家重点研发计划项目（2018YFD1000704）；国家自然科学基金项目（31771721）；国家甘薯产业技术体系项目（CARS-11）作者简介：蒋薇（1996- ），女，江苏常州人，硕士研究生，主要从事甘薯栽培生理与生态研究。（E-mail）1298081288@qq.com通讯作者：唐忠厚，（E-mail）zhonghoutang@sina.com
更新日期/Last Update: 2021-09-06