[1]朱伟峰,谭凯,王芳,等.支气管败血波氏菌截短BcfA表达的重组蛋白及其免疫活性分析[J].江苏农业学报,2021,(03):699-703.[doi:doi:10.3969/j.issn.1000-4440.2021.03.019]
　ZHU Wei-feng,TAN Kai,WANG Fang,et al.Recombinant protein expressed from BcfA in Bordetella bronchiseptica and its immune activity analysis[J].,2021,(03):699-703.[doi:doi:10.3969/j.issn.1000-4440.2021.03.019]
点击复制

支气管败血波氏菌截短BcfA表达的重组蛋白及其免疫活性分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年03期
页码:
699-703
栏目:
畜牧兽医·水产养殖
出版日期:
2021-06-30

文章信息/Info

Title:
Recombinant protein expressed from BcfA in Bordetella bronchiseptica and its immune activity analysis
作者:
朱伟峰1; 2; 3; 谭凯1; 2; 3; 王芳1; 2; 3; 程序1; 2; 3; 仇汝龙1; 2; 3; 范志宇1; 2; 3; 陈露1; 2; 3
（1.江苏省农业科学院兽医研究所，江苏南京210014；2.农业农村部兽用生物制品工程技术重点实验室，江苏南京210014；3.国家兽用生物制品工程技术研究中心，江苏南京210014）
Author(s):
ZHU Wei-feng1; 2; 3; TAN Kai1; 2; 3; WANG Fang1; 2; 3; CHENG Xu1; 2; 3; QIU Ru-long1; 2; 3; FAN Zhi-yu1; 2; 3; CHEN Lu1; 2; 3
(1.Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture and Rural affairs, Nanjing 210014, China;3.National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China)
关键词:
支气管败血波氏菌; 波氏菌黏附因子A（BcfA）; 截短表达; 保护性抗原
Keywords:
Bordetella bronchiseptica; Bordetella adhesion factor A (BcfA); truncated expression; protective antigen
分类号:
S858.291.63
DOI:
doi:10.3969/j.issn.1000-4440.2021.03.019
文献标志码:
A
摘要:
为提高支气管败血波氏菌保护性抗原BcfA重组蛋白的表达量和可溶性，对编码BcfA N端373位氨基酸残基之后的BcfA基因碱基序列进行了克隆和表达，截短后的BcfA表达量提高了10倍，且主要为可溶性表达。Western blot结果显示，截短BcfA表达后的重组蛋白与支气管败血波氏菌感染康复血清反应依然明显。免疫保护力试验结果表明截短BcfA表达的重组蛋白依然对支气管败血波氏菌表现出良好的免疫保护作用。
Abstract:
To improve the expression and solubility of BcfA recombinant protein of a protective antigen against Bordetella bronchiseptica, the base sequence of BcfA gene encoding amino acid residues of the N-terminal after the 373rd position was cloned and expressed. The expression of shortened BcfA was increased by ten times, and the expression form was mainly soluble. Western blot results showed that the truncated protein encoded by BcfA still exhibited obvious reaction with convalescent serum infected by Bordetella bronchiseptica. The results of immunoprotection test showed that recombinant protein expressed from truncated BcfA still showed good immunoprotection against Bordetella bronchiseptica.

参考文献/References:

[1]吴润. 波氏菌属[M]//陆承平. 兽医微生物学. 北京: 中国农业出版社, 2001:275-278.
[2]SAMEED M, SULLIVAN S, DEEPAK J. The curious sough: a case report of cystic lung disease caused by Bordetelia bronchiseptica in a patient with AIDS[J]. Chest, 2018,154(4):138.
[3]MONTI M, DIANO D, ALLEGRINI F, et al. Bordetella bronchiseptica pneumonia in a patient with lung cancer; a case report of a rare infection[J]. BMC Infectious Diseases, 2017,17(1):644.
[4]AI W C, PENG Z, WANG F, et al. A Marker-free Bordetella bronchiseptica aroA/bscN double deleted mutant confers protection against lethal challenge[J]. Vaccines, 2019,7(4): 176.
[5]孟庆文,童光志,韩凌霞. 基因工程疫苗概述[M]//童光志, 王云峰. 动物基因工程疫苗原理与方法. 北京: 化学工业出版社, 2009:81-91.
[6]YOUNT K, JENNINGS-GEE J, CAUTION K, et al. Bordetella colonization factor A (BcfA) elicits protective immunity against Bordetella bronchiseptica in the absence of an additional adjuvant[J]. Infection and Immunity, 2019,87(10): e00506-1910.
[7]SUKUMAR N, LOVE C, CONOVER M, et al. Active and passive immunizations with Bordetella colonization factor A pProtect mice against respiratory challenge with Bordetella bronchiseptica[J]. Infection and Immunity, 2009,77(2):885-895.
[8]范志宇,魏后军,胡波,等. 兔多杀性巴氏杆菌和支气管败血波氏杆菌复合PCR方法的建立及临床应用[J]. 江苏农业学报, 2015,31(2):376-381.
[9]ZHAO Z Q, XUE Y, TANG X B, et al. Immunogenicity of recombinant protective antigen and efficacy against intranasal challenge with Bordetella bronchiseptica[J]. Vaccine, 2009,27(18):2523-2528.
[10]WANG C W, LIU L P, ZHANG Z, et al. Immunological and protective effects of Bordetella bronchiseptica subunit vaccines based on the recombinant N-terminal domain of dermonecrotic toxin[J]. International Immunopharmacology, 2015,28(2SI):952-959.
[11]ZHU W, WU C, KANG C, et al. Evaluation of the protective efficacy of four newly identified surface proteins of Erysipelothrix rhusiopathiae[J]. Vaccine, 2018,36(52):8079-8083.
[12]YANG C Y, PAN H R, WEI M X, et al. Hepatitis E virus capsid protein assembles in 4M urea in the presence of salts[J]. Protein Science, 2013,22(3):314-326.
[13]赵宁,王芳,恽时锋,等. 兔支气管败血波氏杆菌DNT蛋白的分段表达及其免疫保护性研究[J]. 中国预防兽医学报, 2011,33(8):648-652.
[14]LIU Y, CHEN H, WEI Q, et al. Immune efficacy of five novel recombinant Bordetella bronchiseptica proteins[J]. BMC Veterinary Research, 2015,11(1):173.
[15]WANG C W, LIU L P, ZHANG Z, et al. Immunological and protective effects of Bordetella bronchiseptica subunit vaccines based on the recombinant N-terminal domain of dermonecrotic toxin[J]. International Immunopharmacology, 2015,28(2):952-959.
[16]赵战勤,薛云,吴斌,等. 重组百日咳杆菌黏附素蛋白免疫小鼠可完全抵抗支气管败血波氏杆菌的致死性感染[J]. 微生物学报, 2008, 48 (3):337-341.

备注/Memo

备注/Memo:
收稿日期：2020-11-03基金项目：江苏省农业科技自主创新基金项目[CX(19)3016]；江苏省自然科学基金青年基金项目（BK20190270）；财政部和农业农村部国家现代农业产业技术体系资助项目（CARS-43-C-1）作者简介：朱伟峰 (1984-)，男，河北石家庄人，博士，助理研究员，主要从事新型疫苗设计与动物疫病防控基础研究。(Tel)025-84392720；(E-mail)zhuweifeng8@126.com通讯作者：王芳，(E-mail)rwangfang@126.com
更新日期/Last Update: 2021-07-05