[1]彭凌,林锦铨,李玲慧,等.一株猪丹毒丝菌的全基因组测序及SpaA基因分析[J].江苏农业学报,2021,(03):694-698.[doi:doi:10.3969/j.issn.1000-4440.2021.03.018]
　PENG Ling,LIN Jin-quan,LI Ling-hui,et al.Whole-genome sequencing and SpaA gene analysis of a Erysipelothrix rhusiopahiae strain[J].,2021,(03):694-698.[doi:doi:10.3969/j.issn.1000-4440.2021.03.018]
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一株猪丹毒丝菌的全基因组测序及SpaA基因分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年03期
页码:
694-698
栏目:
畜牧兽医·水产养殖
出版日期:
2021-06-30

文章信息/Info

Title:
Whole-genome sequencing and SpaA gene analysis of a Erysipelothrix rhusiopahiae strain
作者:
彭凌; 林锦铨; 李玲慧; 刘博婷; 蔡巩林
（韶关学院英东生物与农业学院，广东韶关512005)
Author(s):
PENG Ling; LIN Jin-quan; LI Ling-hui; LIU Bo-ting; CAI Gong-lin
(Henry Fok College of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China)
关键词:
猪丹毒丝菌; 全基因组测序; 表面保护性抗原A（SpaA）
Keywords:
Erysipelothrix rhusiopahiae; whole-genome sequencing; surface protective antigen A (SpaA)
分类号:
S852.61
DOI:
doi:10.3969/j.issn.1000-4440.2021.03.018
文献标志码:
A
摘要:
本研究测定猪丹毒丝菌临床毒株SG7的全基因组序列，并运用生物信息学方法对测定的全基因组序列及猪丹毒丝菌表面保护性抗原A基因（SpaA）进行分析。SG7菌株的基因组全长为1 834 291.00 bp，G+C含量为36.3%，基因总数1 846个。将SG7菌株与GenBank中8条完整的猪丹毒丝菌全基因组序列进行比较，发现国内外不同菌株间基因组的基本信息存在不同程度差异。基于全基因组单核苷酸多态性（SNPs）的系统发育分析结果表明，9株菌株聚为3个分支，国内菌株并不完全处于同一分支，SG7菌株与国内常见菌株不属于同一进化分支。SpaA基因高变区的分析结果显示，9株菌株亦可分为3个SpaA型，SG7菌株为携带Met203的高致病性菌株。除SG7菌株外，其他8株菌株的SpaA基因分型结果与基于全基因组SNPs分析的结果一致，说明SG7菌株的遗传背景复杂。本研究结果可为猪丹毒丝菌基因组整体水平研究和疫苗的研发奠定基础。
Abstract:
In this study, the whole genome sequence of Erysipelothrix rhusiopahiae clinical strain SG7 was determined, and the whole genome sequence and the surface protective antigen A (SpaA) gene were analyzed using bioinformatics method. The full length of SG7 genome was 1 834 291.00 bp, and the total quantity of the predicted genes were 1 846 with a G+C content of 36.3%. Different degrees of differences of genomic basic information between different strains were found by comparing the complete genome sequences of SG7 and eight E. rhusiopahiae found in GenBank. Phylogenetic analysis based on genome-wide single nucleotide polymorphisms (SNPs) revealed that nine strains could be clustered into three distinct clades, and strains isolated from China were not in the same clade, while SG7 and common strains isolated from China did not belong to the same clade. The analysis results of hypervariable region of SpaA showed that nine strains could also be divided into three SpaA types, and SG7 was a highly pathogenic strain carrying Met203. Except for SG7, the SpaA genotyping results of other eight strains were consistent with the analytic results obtained based on the genome-wide SNPs analysis, which indicated that the genetic background of SG7 was complex. The results can provide data basis for the research at the whole genome level and development of E. rhusiopahiae vaccine.

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备注/Memo

备注/Memo:
收稿日期：2020-09-07基金项目：广东省自然科学基金项目（2017A030307041）；国家级大学生创新创业训练计划项目（201910576002）作者简介：彭凌（1975-），男，江西奉新人，硕士，副教授，主要从事动物病原微生物及分子生物学研究。（E-mail）308668576@qq.com
更新日期/Last Update: 2021-07-05