[1]高营,林云,袁星星,等.蚕豆VfGASA1基因的异源过表达延迟拟南芥开花[J].江苏农业学报,2021,(01):44-52.[doi:doi:10.3969/j.issn.1000-4440.2021.01.006]
 GAO Ying,LIN Yun,YUAN Xing-xing,et al.Heterologous overexpression of Vicia faba VfGASA1 gene delays flowering in transgenic Arabidopsis[J].,2021,(01):44-52.[doi:doi:10.3969/j.issn.1000-4440.2021.01.006]
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蚕豆VfGASA1基因的异源过表达延迟拟南芥开花()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2021年01期
页码:
44-52
栏目:
遗传育种·生理生化
出版日期:
2021-02-28

文章信息/Info

Title:
Heterologous overexpression of Vicia faba VfGASA1 gene delays flowering in transgenic Arabidopsis
作者:
高营12林云2袁星星2薛晨晨2陈新2朱月林1
(1.南京农业大学园艺学院,江苏南京210095;2.江苏省农业科学院经济作物研究所,江苏南京210014)
Author(s):
GAO Ying12 LIN Yun2 YUAN Xing-xing2 XUE Chen-chen2 CHEN Xin2 ZHU Yue-lin1
(1.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;2.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
蚕豆VfGASA1拟南芥开花时间赤霉素
Keywords:
Vicia fabaVfGASA1Arabidopsisflowering timegibberellin
分类号:
S643.6
DOI:
doi:10.3969/j.issn.1000-4440.2021.01.006
文献标志码:
A
摘要:
GASA(Gibberellic acid-stimulated in Arabidopsis)蛋白是一类受赤霉素调控的小分子蛋白质,参与植物生长发育的多条途径。本研究在蚕豆(Vicia faba)中同源克隆了VfGASA1基因,该基因的开放阅读框全长为363 bp,编码1个包含120个氨基酸残基的蛋白质,VfGASA1在蚕豆叶片和嫩荚中高表达,亚细胞定位结果显示,VfGASA1是一个定位于质外体的分泌蛋白。异源过表达VfGASA1导致转基因拟南芥开花延迟、莲座叶数量增多,外施赤霉素能够恢复这一现象。实时荧光定量结果显示,转基因拟南芥植株中FT基因显著下调,而DELLA基因中的GAI基因显著上调,说明VfGASA1可能是通过间接抑制DELLA蛋白的表达从而调控植物开花。本研究结果为蚕豆的开花调控育种提供了理论依据。
Abstract:
Gibberellic acid-stimulated in Arabidopsis (GASA) protein is a kind of protein with small molecular weight regulated by gibberellin and participates in various pathways of plant growth and development. In this study, the GASA gene VfGASA1 in faba bean (Vicia faba) was got by homologous cloning. The open reading frame(ORF) of VfGASA1 was 363 bp in length and encoded a protein containing 120 amino acid residues. VfGASA1 was highly expressed in the leaves and capsules of faba beans, and subcellular localization showed that VfGASA1 was a secretory protein located in the apoplast. Heterologous overexpression of VfGASA1 in Arabidopsis could delay flowering and increase the number of rosette leaves, while the phenomenon could be recovered by the application of exogenous gibberellin. Results of real-time PCR (RT-PCR) showed that the expressional level of ‘florigen’ gene (FT) was significantly down-regulated in transgenic Arabidopsis while GAI (one of DELLA genes) was significantly up-regulated. Therefore, VfGASA1 might regulate the flowering of plants by indirect inhibiting the expression of DELLA genes. This study provides theoretic basis for breeding programs targeted on the flowering control of faba bean.

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备注/Memo

备注/Memo:
收稿日期:2020-04-29基金项目:国家食用豆产业技术体系生物防治与综合防控岗位科学家项目(CARS-08-G15);江苏特粮特经产业技术体系集成创新中心项目[JATS(2019)399]作者简介:高营(1994-),女,河南南阳人,硕士研究生,从事豆类作物分子育种研究。(E-mail)3099618289@qq.com通讯作者:朱月林,(E-mail)ylzhu@njau.edu.cn
更新日期/Last Update: 2021-03-15