[1]孙萌萌,王莹慧,汪育文,等.南粳5055及其亲本的光合特性[J].江苏农业学报,2020,(01):1-9.[doi:doi:10.3969/j.issn.1000-4440.2020.01.001]
　SUN Meng-meng,WANG Ying-hui,WANG Yu-wen,et al.Photosynthetic characteristics of japonica rice cultivar Nanjing 5055 and its parents[J].,2020,(01):1-9.[doi:doi:10.3969/j.issn.1000-4440.2020.01.001]
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南粳5055及其亲本的光合特性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2020年01期
页码:
1-9
栏目:
遗传育种·生理生化
出版日期:
2020-02-29

文章信息/Info

Title:
Photosynthetic characteristics of japonica rice cultivar Nanjing 5055 and its parents
作者:
孙萌萌1; 2; 王莹慧1; 汪育文1; 陈国祥1; 赵凌3; 张亚东3
(1.南京师范大学生命科学学院，江苏南京210023；2.南京农业大学农学院，江苏南京210014；3.江苏省农业科学院粮食作物研究所，江苏南京210014)
Author(s):
SUN Meng-meng1; 2; WANG Ying-hui1; WANG Yu-wen1; CHEN Guo-xiang1; ZHAO Ling3; ZHANG Ya-dong3
(1. College of Life Sciences, Nanjing Normal University, Nanjing 210023, China;2.College of Agriculture, Nanjing Agricultural University, Nanjing 210014, China;3.Institute of Food&Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
优良食味粳稻; 叶绿素荧光参数; 类囊体膜蛋白复合物
Keywords:
japonica rice with good taste quality; chlorophyll fluorescence parameters; thylakoid membrane protein complexes
分类号:
S511.01
DOI:
doi:10.3969/j.issn.1000-4440.2020.01.001
文献标志码:
A
摘要:
为探究优良食味粳稻的光合特性，进一步挖掘南粳系列水稻光合潜力，对优良食味粳稻南粳5055及其母本武粳13和父本日本优质粳稻关东194的叶绿素荧光参数、类囊体膜蛋白组分与含量进行分析。结果显示：南粳5055在生殖生长期的叶绿素和类胡萝卜素含量、净光合速率、量子产额、光合性能指数高于关东194，略低于武粳13；南粳5055在完熟期的结实率与千粒质量接近武粳13，高于关东194；温和蓝绿胶电泳结果表明，南粳5055在孕穗期和灌浆期光系统I复合物含量较高；蛋白质免疫印迹显示南粳5055具有较高的Lhca1和Lhca2蛋白含量，而光系统II中心复合物和捕光色素II复合物等其他复合物含量均低于武粳13。说明南粳5055的光合特性在一定程度上与母本武粳13较为接近，南粳5055在孕穗期和乳熟期保持相对较高的光系统I复合物含量，且在生育后期具有高效的热耗散能力可以避免光抑制的发生，推测这可能是该品种能保持高效光合特性的物质和代谢基础。
Abstract:
In order to explore the photosynthetic characteristics and photosynthetic potentiality of Nanjing rice, the chlorophyll fluorescence parameters and the components and contents of thylakoid membrane protein of Nanjing 5055, its female parent Wujing 13 and male parent Guandong 194 were studied. The results showed that the chlorophyll content, carotenoid content, net photosynthesis rate, quantum yield and photosynthetic performance index of Nanjing 5055 were higher than those of Guandong 194 and slightly lower than those of Wujing 13 in the reproductive period. The seed setting rate and 1000-grain weight of Nanjing 5055 were higher than those of Guandong 194 and chose to those of Wujing 13 in the full stage. Blue native polyacrylamide gel electrophoresis (BN-PAGE) results showed that the contents of photosystem I complexes in Nanjing 5055 were higher at the booting and milking stages. The results of western blotting indicated that the contents of Lhca1 and Lhca2 protein in Nanjing 5055 were higher, while the contents of other complexes such as photosystem II core and light-harvesting pigment II were slightly lower than those in Wujing 13. The above results indicated that the photosynthetic performance of Nanjing 5055 was close to its female parent Wujing 13, and the contents of photosystem I complexes were higher in the booting and milk stages. Moreover, Nanjing 5055 had high heat dissipation performance to avoid light inhibition in the later growth stage, so it was speculated that those were material and metabolic basis for Nanjing 5055 to maintain high photosynthetic efficiency.

参考文献/References:

[1]欧志英, 彭长连, 阳成伟, 等. 超高产水稻剑叶的高效光合特性[J]. 热带亚热带植物学报, 2003, 11(1):1-6.
[2]剧成欣, 陶进, 钱希旸, 等. 不同年代中籼水稻品种的叶片光合性状[J]. 作物学报, 2016, 42(3):415-426.
[3]WEI X D. Photosynthesis enhanced oxidative stress tolerance in high-yield rice varieties (Oryza sativa var. japonica L.) in the field[J]. Genetics&Molecular Research, 2016, 15(3):1-14.
[4]刘红江, 陈虞雯, 张岳芳, 等. 不同播栽方式对水稻叶片光合特性及产量的影响[J]. 江苏农业学报, 2016, 32(6):1206-1211.
[5]李晓峰, 程金秋, 梁健, 等. 秸秆全量还田与氮肥运筹对机插粳稻产量及氮素吸收利用的影响[J]. 作物学报, 2017, 43(6):912-924.
[6]周根友, 翟彩娇, 邓先亮, 等. 盐逆境对水稻产量、光合特性及品质的影响[J]. 中国水稻科学, 2018, 32(2):146-154.
[7]叶露幻, 沈唯军, 郑宝刚, 等. 两优培九剑叶衰老过程光合膜功能及蛋白质复合物的变化[J]. 作物学报, 2013, 39(11):2030-2038.
[8]ARNON D I. Copper enzymes in isolated chloroplasts: polyphenoloxidase in Beta vulgaris[J]. Plant Physiology, 1949, 24(1):1.
[9]LICHTENTHALER H, LICHTENTHALER H K. Lichtenthaler,chlorophylls and carotenoids: pigment photosynthetic biomembranes[J]. Methods in Enzymology, 1987, 148(1):350-382.
[10]STRASSER R J, TSIMILLI-MICHAEL M. Activity and heterogeneity of PS II probed in vivo by the chlorophyll a fluorescence rise O-[K]-J-I-P[J].Photosynthesis: Mechanisms and Effects,1998, 5(1):4321-4324.
[11]WANG Y, ZHANG J, YU J, et al. Photosynthetic changes of flag leaves during senescence stage in super high-yield hybrid rice LYPJ grown in field condition[J]. Plant Physiology and Biochemistry, 2014, 82(3):194-201.
[12]KGLER M, JNSCH L, KRUFT V, et al. Analysis of the chloroplast protein complexes by blue-native polyacrylamide gelelectrophoresis (BN-PAGE)[J]. Photosynthesis Research, 1997, 53(1):35-44.
[13]SHAO J, ZHANG Y, YU J, et al. Isolation of thylakoid membrane complexes from rice by a new double-strips BN/SDS-PAGE and bioinformatics prediction of stromal ridge subunits interaction.[J]. PLoS ONE, 2011, 6(5):e20342.
[14]SHEN W J,CHEN G X, XU J G, et al. High light acclimation of Oryza sativa L. leaves involves specific photosynthetic-sourced changes of NADPH/NADP+ in the midvein.[J]. Protoplasma, 2015, 252(1):77-87.
[15]牛东东, 郝育杰, 荣瑞娟, 等. 转基因水稻中GUS蛋白质的检测及其表达特征[J]. 中国农业科学, 2014, 47(14):2715-2722.
[16]HOLLAND V, KOLLER S, BRGGEMANN W. Insight into the photosynthetic apparatus in evergreen and deciduous european oaks during autumn senescence using OJIP fluorescence transient analysis[J]. Plant Biology, 2014, 16(4):801-808.
[17]SRIVASTAVA A, STRASSER R J. Greening of peas: parallel measurements of 77 K emission spectra, OJIP chlorophylla fluorescence transient, period four oscillation of the initial fluorescence level, delayed light emission, and P700[J]. Photosynthetica, 1999, 37(3):365-392.
[18]张笑寒, 赵德刚, 何友勋, 等. 杂交水稻毕粳优210及其亲本光合特性研究[J]. 云南农业大学学报, 2016, 31(3):381-386.
[19]刘自刚, 孙万仓, 方彦, 等. 夜间低温对白菜型冬油菜光合机构的影响[J]. 中国农业科学, 2015, 48(4):672-682.
[20]张菂, 陈昌盛, 李鹏民, 等. 利用快速荧光、延迟荧光和820 nm光反射同步测量技术探讨干旱对平邑甜茶叶片光合机构的伤害机制[J]. 植物生理学报, 2013, 49(6):551-560.
[21]ZHANG Z S, LI G, GAO H Y, et al. Characterization of photosynthetic performance during senescence in stay-green and quick-leaf-senescence,Zea mays L. inbredlines[J]. PLoS ONE, 2012, 7(8):e42936.
[22]孙小玲, 许岳飞, 马鲁沂, 等.植株叶片的光合色素构成对遮阴的响应[J]. 植物生态学报, 2010, 34(8):989-999.
[23]李霞, 焦德茂. 水稻耐光氧化和耐荫特性的生理基础(英文)[J]. 植物学报, 2000, 42(12):1271-1277.
[24]MORALES F, ABADIA J, ABADIA A. Thermal energy dissipation in plants under unfavorable soil conditions[M]// ADAMS B, GARAB G, ADAMS W. Non-photochemical quenching and energy dissipation in plants, algae and cyanobacteria.New York: Springer Dordrecht HeidelberPress, 2014.
[25]孟力力,柳军,皮杰,等. 不同光质LED光源对彩叶草生长及叶绿素荧光参数的影响[J]. 江苏农业科学,2018,46(20):161-164.
[26]BAKER N R. Chlorophyll fluorescence: a probe of photosynthesis in vivo[J]. Annual Review of Plant Biology, 2008, 59(1):89-113.
[27]王玉丽，孙居文，荀守华，等. 干旱胁迫对东岳红光合特性、叶绿素荧光参数及叶片相对含水量的影响[J]. 山东农业科学,2017,49(4):46-50.
[28]ZHANG M P, ZHANG C J, YU G H, et al. Changes in chloroplast ultrastructure, fatty acid components of thylakoid membrane and chlorophyll a fluorescence transient in flag leaves of a super-high-yield hybrid rice and its parents during the reproductive stage[J]. Journal of Plant Physiology, 2010, 167(4):277-285.
[29]杨万基，蒋欣梅，高欢，等. 28-高芸苔素内酯对低温弱光胁迫辣椒幼苗光合和荧光特性的影响[J]. 南方农业学报，2018，49（4）：741-747.
[30]程占慧, 刘良云. 基于叶绿素荧光发射光谱的光能利用率探测[J]. 农业工程学报, 2010, 26(增刊2): 74-80.
[31]黄相玲．福清龙江不同断面空心莲子草生理生态特征比较［J］．杂草学报，2018,36(1):20-24.
[32]DEKKER J P, BOEKEMA E J. Supramolecular organization of thylakoid membrane proteins in green plants[J]. BBA-Bioenergetics, 2005, 1706(1):12-39.
[33]ZHOU F, LIU S, HU Z H, et al. Effect of monogalactosyldiacylglycerol on the interaction between photosystem II core complex and its antenna complexes in liposomes of thylakoid lipids[J]. Photosynthesis Research, 2009, 99(3):185-193.
[34]OUKARROUM A, SCHANSKER G, STRASSER R J. Drought stress effects on photosystem I content and photosystem II thermotolerance analyzed using Chl a fluorescence kinetics in barley varieties differing in their drought tolerance[J]. Physiol Plant, 2009, 137:188-199.
[35]RUBAN A V, YOUNG A J, PASCAL A A, et al. The effects of illumination on the xanthophyll composition of the photosystem II light-harvesting complexes of spinach thylakoid membranes[J]. Plant Physiol, 1999, 104:227-234.

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备注/Memo

备注/Memo:
收稿日期：2019-05-10基金项目：江苏省农业科技自主创新基金项目[CX(17)3022]；江苏省自然科学基金(青年科技人才专项基金)项目(BK20171034)；江苏高校优势学科建设工程项目(PAPD)作者简介：孙萌萌(1991-)，女，山东滨州人，硕士研究生，研究方向为水稻光合生理生化。(Tel) 18260029722；(E-mail) smmnjnnu@163.com通讯作者：陈国祥，(Tel) 025-85891079；(E-mail) gxchen@njnu.edu.cn
更新日期/Last Update: 2020-03-13