[1]汤纯,史云娇,卞欢,等.食源性克雷伯氏菌的分离鉴定与生物膜形成特性[J].江苏农业学报,2019,(05):1216-1221.[doi:doi:10.3969/j.issn.1000-4440.2019.05.031]
　TANG Chun,SHI Yun-jiao,BIAN Huan,et al.Isolation，identification and biofilm formation characteristics of Klebsiella species in fresh duck blood[J].,2019,(05):1216-1221.[doi:doi:10.3969/j.issn.1000-4440.2019.05.031]
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食源性克雷伯氏菌的分离鉴定与生物膜形成特性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年05期
页码:
1216-1221
栏目:
加工贮藏·质量安全
出版日期:
2019-10-31

文章信息/Info

Title:
Isolation，identification and biofilm formation characteristics of Klebsiella species in fresh duck blood
作者:
汤纯1; 2; 史云娇1; 2; 卞欢1; 孙芝兰1; 刘芳1; 朱云龙2
(1.江苏省农业科学院农产品加工研究所，江苏南京210014；2.扬州大学旅游烹饪学院，江苏扬州225127)
Author(s):
TANG Chun1; 2; SHI Yun-jiao1; 2; BIAN Huan1; SUN Zhi-lan1; LIU Fang1; ZHU Yun-long2
(1.Institute of Agricultural Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；2.College of Tourism and Cuisine, Yangzhou University, Yangzhou 225127, China)
关键词:
新鲜鸭血; 克雷伯氏菌; 生物膜; 胞外多糖
Keywords:
fresh duck blood; Klebsiella sp.; biofilm; exopolysaccharides
分类号:
TS251.7
DOI:
doi:10.3969/j.issn.1000-4440.2019.05.031
文献标志码:
A
摘要:
为探究新鲜鸭血中的优势腐败菌及其生物膜成膜特性，通过传统分离培养法分离获得59株腐败菌，经16S rRNA序列鉴定发现肠杆菌是其中的优势菌，并且克雷伯氏菌是其中的优势腐败菌。通过结晶紫染色法检测了所分离肠杆菌的生物膜形成能力，发现1株克雷伯氏菌K6的成膜能力最强。通过分析该菌在不同培养阶段生物膜内的菌数及胞外多糖含量变化，发现该菌在培养3 d和5 d时其生物膜内菌数和胞外多糖含量分别达到最高值，进一步证实了克雷伯氏菌较强的成膜特性。本研究结果表明在鸭血加工过程中应该对克雷伯氏菌进行重点控制。
Abstract:
To explore the composition and biofilm formation characteristics of dominant spoilage bacteria isolated from fresh duck blood, fifty-nine strains were isolated by the culture-dependent method and formation identified by 16S rRNA gene analysis. The results showed that Klebsiella was one of the dominant spoilage bacteria. The biofilm-forming ability of the isolated stains was determined by crystal violet staining, and it was found that one strain of Klebsiella K6 had the strongest biofilm-forming ability. The number of bacteria in the biofilm and content of extracellular polysaccharide reached the highest value at 3 d and 5 d, respectively. The results of this study indicated that Klebsiella had strong biofilm-forming ability and should be controlled during duck blood processing.

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备注/Memo

备注/Memo:
收稿日期：2019-01-17 基金项目：国家自然科学基金面上项目(31871866)；国家重点研发计划项目(2016YFC1300200) 作者简介：汤纯(1995-)，女，江苏镇江人，硕士研究生，研究方向为营养与食品卫生。(E-mail)826034822@qq.com 通讯作者：刘芳，(E-mail)fangliu82@163.com；朱云龙，(E-mail)498051574@qq.com
更新日期/Last Update: 2019-11-11