[1]杨宗英,房文红,周俊芳,等.高效液相色谱/串联质谱法研究溴氰菊酯在中华绒螯蟹体内的富集消除规律[J].江苏农业学报,2019,(03):709-715.[doi:doi:10.3969/j.issn.1000-4440.2019.03.029]
　YANG Zong-ying,FANG Wen-hong,ZHOU Jun-fang,et al.Study on the accumulation and elimination of deltamethrin in Eriocheir sinensis by high performance liquid chromatography/tandem mass spectrometry[J].,2019,(03):709-715.[doi:doi:10.3969/j.issn.1000-4440.2019.03.029]
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高效液相色谱/串联质谱法研究溴氰菊酯在中华绒螯蟹体内的富集消除规律()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年03期
页码:
709-715
栏目:
加工贮藏·质量安全
出版日期:
2019-06-30

文章信息/Info

Title:
Study on the accumulation and elimination of deltamethrin in Eriocheir sinensis by high performance liquid chromatography/tandem mass spectrometry
作者:
杨宗英1; 2; 房文红1; 周俊芳1; 胡鲲3; 杨先乐3
（1.农业部东海与远洋渔业资源开发利用重点实验室，上海200090；2.南昌市农业科学院，江西南昌330038；3.上海海洋大学国家水生动物病原库，上海201306）
Author(s):
YANG Zong-ying1; 2; FANG Wen-hong1; ZHOU Jun-fang1; HU Kun3; YANG Xian-le3
(1.Key Laboratory of East China Sea & Oceanic Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Shanghai 200090, China;2.Nanchang Academy of Agricultural Sciences, Nanchang 330038, China;3.State Collection Center of Aquatic Pathogen, Shanghai Ocean University, Shanghai 201306, China)
关键词:
中华绒螯蟹; 溴氰菊酯; 富集消除规律; 液相色谱/串联质谱法
Keywords:
Eriocheir sinensis; deltamethrin; rule of accumulation and elimination; liquid chromatography/tandem mass spectrometry
分类号:
S966.16
DOI:
doi:10.3969/j.issn.1000-4440.2019.03.029
文献标志码:
A
摘要:
本研究建立了中华绒螯蟹（Eriocheir sinensis）体内溴氰菊酯残留的液相色谱/串联质谱检测方法，并选择溴氰菊酯对中华绒螯蟹的安全质量浓度066 μg/L，在（20±1） ℃下，对中华绒螯蟹进行浸浴，研究溴氰菊酯在中华绒螯蟹体内的富集消除规律。样品经乙腈提取，采用电喷雾正离子源（ESI+）和多重反应监测（MRM）模式测定，基质匹配标准曲线定量。结果表明，溴氰菊酯标准曲线在01～500 μg/L质量浓度线性关系良好，决定系数为0999 8，检测限为0035 μg/L。010 μg/kg（μg/L）、1000 μg/kg（μg/L）、2000 μg/kg（μg/L）和5000 μg/kg（μg/L）添加水平下，溴氰菊酯回收率为8263%～10100%。各组织对溴氰菊酯的富集能力不同，其中血淋巴和鳃富集能力较强。血淋巴、肝胰腺和鳃等组织中药物质量浓度同时达到峰值，达到峰值时间（Tmax）为05 h，峰值质量浓度或质量分数（Cmax）分别为2780 μg/L，0567 μg/kg和1165 μg/kg。为了最大限度地避免溴氰菊酯残留对人体造成的危害，除了选择中华绒螯蟹重要的可食组织肝胰腺作为检测对象外，还应选择血淋巴等组织进行进一步的药残检测。
Abstract:
A method for the determination of deltamethrin in Eriocheir sinensis by liquid chromatography/tandem mass spectrometry was established. The crabs were given a immersion bath in the safe concentration of deltamethrin at the dose of 066 μg/L and temperature of (20±1) ℃ to investigate the rule of accumulation and elimination of deltamethrin in E sinensis The samples were extracted by acetonitrile Electrospray positive ion source (ESI+), multiple reaction monitoring mode (MRM) and matrixmatched external standard method were applied to sample analysis Results indicated that the standard curve of deltamethrin was in good linearity in the range of 0.1-50.0 μg/L, the coefficient of determination was 0999 8, and the detection limit was 0035 μg/L The recoveries for deltamethrin at the addition of 010 μg/kg(μg/L), 1000 μg/kg(μg/L), 2000 μg/kg(μg/L) and 5000 μg/kg(μg/L) ranged from 8263% to 10100% The enrichment capacities of different tissues of E sinensis for deltamethrin were different, the capacities of hemolymph and gills were comparatively stronger The concentrations of deltamethrin in hemolymph, hepatopancreas and gills reached their Cmax(2780 μg/L, 0567 μg/kg and 1165 μg/kg) at the same time at 05 h (Tmax) In order to avoid the harm caused by deltamethrin residue to the human body, the hemolymph and other tissues should be also selected as the object of drug residue detection except for hepatopancreas, which is the important edible tissue of E. sinensis.

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备注/Memo

备注/Memo:
收稿日期：2018-09-11 基金项目：农业部东海与远洋渔业资源开发利用重点实验室开放课题 作者简介：杨宗英（1988-），女，河南驻马店人，博士，工程师，研究方向为水产生物免疫学与病害控制。（E-mail） lxyangzongying@163.com 通讯作者：胡鲲，（E-mail）khu@shou.edu.cn
更新日期/Last Update: 2019-06-30