[1]李隐侠,冯小品,张莉,等.热应激前后湖羊下丘脑差异表达新基因的筛选与注释[J].江苏农业学报,2019,(02):363-369.[doi:doi:10.3969/j.issn.1000-4440.2019.02.017]
　LI Yin-xia,FENG Xiao-pin,ZHANG Li,et al.Screening and annotation of novel genes differentially expressed in hypothalamus before and after heat stress in Hu sheep[J].,2019,(02):363-369.[doi:doi:10.3969/j.issn.1000-4440.2019.02.017]
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热应激前后湖羊下丘脑差异表达新基因的筛选与注释()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年02期
页码:
363-369
栏目:
畜牧兽医·水产养殖
出版日期:
2019-04-30

文章信息/Info

Title:
Screening and annotation of novel genes differentially expressed in hypothalamus before and after heat stress in Hu sheep
作者:
李隐侠1; 2; 3; 冯小品1; 2; 3; 张莉1; 2; 3; 张俊1; 2; 3; 钱勇1; 2; 3; 孟春花1; 2; 3; 王慧利1; 2; 3; 仲跻峰1; 2; 3; 曹少先1; 2; 3
(1.江苏省农业科学院畜牧研究所，江苏南京210014；2.江苏省农业种质资源保护与利用平台，江苏南京210014；3.农业部种养结合重点实验室，江苏南京210014）
Author(s):
LI Yin-xia1; 2; 3; FENG Xiao-pin1; 2; 3; ZHANG Li1; 2; 3; ZHANG Jun1; 2; 3; QIAN Yong1; 2; 3; MENG Chun-hua1; 2; 3; WANG Hui-li1; 2; 3; ZHONG Ji-feng1; 2; 3; CAO Shao-xian1; 2; 3
（1.Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；2.The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Nanjing 210014, China；3.Key labor atory of Crop and Animal Integrated Farming, Ministry of Agriculture， Nanjing 210014, China)
关键词:
湖羊; 热应激; 下丘脑; 转录组测序; real-time PCR
Keywords:
Hu sheep; heat stress; hypothalamus; transcriptome sequencing; real-time PCR
分类号:
Q781
DOI:
doi:10.3969/j.issn.1000-4440.2019.02.017
文献标志码:
A
摘要:
湖羊是中国南方唯一的绵羊品种，耐湿热是其重要的特色性状之一。但目前关于其耐湿热机制尚不清楚。本研究以湖羊为研究对象，通过转录组测序技术鉴定热应激前后下丘脑组织中的新基因及其差异表达，并通过不同数据库比对和分析，进行新基因功能注释。结果表明，热应激前后在湖羊下丘脑组织中共检测到1 990个新转录本，通过功能注释，发现1 344个新基因。在热应激组和对照组湖羊中，差异表达新基因186个，其中130个新基因表达上调，56个新基因表达下调，通过比对发现这些基因分别与细胞免疫、繁殖、生长代谢及相关信号通路相关。Real-time PCR验证试验结果表明，10个热应激前后表达差异基因中有9个与转录组测序结果一致。这些结果为绵羊基因组的进一步完善及基因功能的挖掘提供了一定的数据基础，为全面解析绵羊热应激的分子机制提供更多的依据。
Abstract:
Hu sheep is the only sheep breed in southern China, and humidity and heat resistance is one of its important characteristics. However, the molecular mechanism of heat stress is still unclear. In this study, Hu sheep was used as the research object to identify new genes and their differential expression in hypothalamus tissue before and after heat stress by transcriptome sequencing technology, ant the new genes were annotated by comparison and analysis of different databases. The results showed that a total of 1 990 new transcripts were detected in the hypothalaum of Hu sheep before and after heat stress, and 1 344 new genes were discovered through functional annotation. In the heat stress group and control group, 186 differentially expressed new genes were identified, of which 130 new genes were up-regulated and 56 genes were down-regulated. These genes were found to be associated with cellular immunity, reproduction, growth and metabolism, and related signaling pathways. Real-time PCR results showed that nine of the ten differentially expressed genes before and after heat stress were consistent with the results of transcriptome sequencing. These results provide a basis for further improvement of sheep genome and excavation of gene function, and provide more basis for comprehensive analysis of the molecular mechanism of heat stress in sheep.

参考文献/References:

[1]黄艳,周阿容,池文文,等. 果蝇热应激生理机制研究进展[J]. 南方农业学报，2018，49（5）：912-921.
[2]HULME P E. Adapting to climate change: is there scope for ecological management in the face of a global threat [J]. Journal of Applied Ecology, 2005, 42:784-294.
[3]MCMANUS C, HERMUCHE P, PAIVA S R, et al. Geographical distribution of sheep breeds in brazil and their relationship with climatic and environmental factors as risk classification for conservation [J]. Brazilian Journal Science and Technology, 2014, 1:1-15.
[4]刘梅.急性热应激对肉仔鸡生长性能及脂肪代谢的影响[J].动物营养学报，2011，23（5）：862-868.
[5]唐姣玉，谢宇洁，刘兆辉，等. 大蒜素对热应激湘黄鸡生产性能及营养物质代谢的影响[J]. 江苏农业学报，2017，33（3）：638-641.
[6]TU W L, CHENG C Y, WANG S H, et al. Profiling of differential gene expression in the hypothalamus of broiler-type Taiwan country chickens in response to acute heat stress [J]. Theriogenology, 2016, 85(3):483-494.
[7]MARAI I F M, El-DARAWANY A A, FADIEL A, et al. Physiological traits as affected by heat stress in sheep—a review [J].Small Ruminant Research, 2007, 71:1-12.
[8]FINOCCHIARO R, VAN KAAM J B C H M, PORTOLANO B, et al. Effect of heat stress on production of Mediterranean dairy sheep [J].Journal of Dairy Science, 2005, 88:1855-1864.
[9]HAMMEL H T, JACKSON D C, STOLWIJK J A, et al. Temperature regulation by hypothalamic proportional control with an adjustable set point [J]. Journal of Applied Physiology, 1963, 18:1146-1154.
[10]MILLER D B, O′CALLAGHAN J P. Neuroendocrine aspects of the response to stress [J]. Metabolism, 2002, 51:5-10.
[11]DA COSTA M J, DA SOLVA R G, DE SOUZA R C. Effect of air temperature and humidity on ingestive behavior of sheep [J]. International Journal of Biometeorology, 1992, 36(4):218-222.
[12]李隐侠，王慧利，邓唯，等. 高温环境下湖羊直肠温度和血液生化指标的监测 [J]. 中国畜牧兽医，2018,45（1）：271-277.
[13]MARAI I F M, AYYAT M S, ABD El-MONEM U M. Growth performance and reproductive traits at first parity of New Zealand White female rabbits as affected by heat stress and its alleviation, under Egyptian conditions [J]. Tropical Animal Health and Production, 2001，33:451-462.
[14]FUQUAY J W. Heat stress as it affects animal production [J].Journal of Animal Science, 1981, 52(1):164-174.
[15]SRIKANDAKUMAR A, JOHNSON E H, MAHGOUB O. Effect of heat stress on respiratory rate, rectal temperature and blood chemistry in Omani and Australian Merino sheep [J]. Small Ruminant Research, 2003，49:193-198.
[16]李隐侠，张俊，钱勇，等. 乌骨绵羊NR5A2基因编码区序列克隆及特征分析 [J]. 江苏农业学报，2014， 30（6）：1375-1382.
[17]张春香，张国林，郭丽娜，等. 基于高通量转录组测序的山羊睾丸和附睾头差异表达基因分析 [J]. 畜牧兽医学报，2014, 45（3）：391-401.
[18]FEDER M E, HOFMANN G E. Heat-shock proteins, molecular chaperones, and the stress response: evolutionary and ecological physiology [J]. Annual Review of Physiology, 1999, 61:243-282.
[19]KREGEL K C. Heat shock proteins: modifying factors in physiological stress responses and acquired thermotolerance [J]. Journal of Applied Physiology, 2002, 92(5):2177-2186.
[20]ALEMU T W, PANDEY H O, SALILEW WONDIM D, et al. Oxidative and endoplasmic reticulum stress defense mechanisms of bovine granulosa cells exposed to heat stress [J].Theriogenology, 2018, 110:130-141.
[21]PRAGNA P, SEJIAN V, BAGATH M, et al. Comparative assessment of growth performance of three different indigenous goat breeds exposed to summer heat stress [J].Journal of Animal Physiology and Animal Nutrition (Berl), 2018, 102(4): 825-836.
[22]RAHMAN M B, SCHELLANDER K, LUCENO N L, et al. Heat stress responses in spermatozoa: Mechanisms and consequences for cattle fertility [J].Theriogenology, 2018, 113:102-112.
[23]GANESAN S, SUMMERS C M, PEARCE S C, et al. Short-term heat stress altered metabolism and insulin signaling in skeletal muscle [J].Journal of Animal Science, 2018, 96(1):154-167.
[24]SAEED-ZIDANE M, LINDEN L, SALILEW-WONDIM D, et al. Cellular and exosome mediated molecular defense mechanism in bovine granulosa cells exposed to oxidative stress [J]. PLoS ONE, 2017 ,12(11):e0187569.
[25]WADA T, PENNINGER J M. Mitogen-activated protein kinases in apoptosis regulation [J]. Oncogene, 2004, 23(16):2838-2849.
[26]RAHMAN M B, VANDAELE L, RIJSSELAERE T, et al. Bovine spermatozoa react to in vitro heat stress by activating the mitogen-activated protein kinase 14 signalling pathway [J]. Reproduction Fertility and Development, 2014, 26(2):245-257.

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备注/Memo

备注/Memo:
收稿日期：2018-07-16 基金项目：国家自然科学基金项目（31501934）；江苏省农业科技自主创新基金项目[CX(18)3004]；江苏现代农业（肉羊）产业技术体系项目[JATS(2018)258] 作者简介：李隐侠(1979-)，女，河南固始人，博士，副研究员，主要从事动物遗传育种与繁殖方面的研究。（E-mail） liyxmh@126.com 通讯作者：曹少先，（E-mail）caoshaoxian@163.com
更新日期/Last Update: 2019-05-05