[1]徐建伟,张小均,李志博,等.幼苗期大田棉花耐旱性的叶绿素荧光和光合特性辅助鉴定[J].江苏农业学报,2019,(01):1-8.[doi:doi:10.3969/j.issn.1000-4440.2019.01.001]
　XU Jian-wei,ZHANG Xiao-jun,LI Zhi-bo,et al.Assistant identification of chlorophyll fluorescence and photosynthesis in cotton seedlings with drought-tolerance under field condition[J].,2019,(01):1-8.[doi:doi:10.3969/j.issn.1000-4440.2019.01.001]
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幼苗期大田棉花耐旱性的叶绿素荧光和光合特性辅助鉴定()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2019年01期
页码:
1-8
栏目:
遗传育种·生理生化
出版日期:
2019-02-26

文章信息/Info

Title:
Assistant identification of chlorophyll fluorescence and photosynthesis in cotton seedlings with drought-tolerance under field condition
作者:
徐建伟1; 2; 张小均1; 3; 李志博1; 3; 魏亦农1; 3
（1.石河子大学农学院，新疆石河子832003；2.石河子大学教务处，新疆石河子832003；3.新疆生产建设兵团绿洲生态农业重点实验室，新疆石河子832003）
Author(s):
XU Jian-wei1; 2; ZHANG Xiao-jun1; 3; LI Zhi-bo1; 3; WEI Yi-nong1; 3
（1.College of Agronomy, Shihezi University, Shihezi 832003, China;2.Teaching Administration Section, Shihzi University, Shihezi 832003, China;3.The Key Laboratory of Oasis Eco-agriculture of Xinjiang Production and Construction Group, Shihezi 832003, China）
关键词:
棉花; 光合作用; 叶绿素荧光; 耐旱性; 鉴定方法
Keywords:
cotton; photosynthesis; chlorophyll fluorescence; drought tolerance; identification method
分类号:
S562.01
DOI:
doi:10.3969/j.issn.1000-4440.2019.01.001
文献标志码:
A
摘要:
为了建立大田棉花耐旱性叶绿素荧光和光合特性的辅助鉴定方法，以北疆棉区2类不同耐旱基因型的4个棉花品种为材料，在大田条件下研究了棉花幼苗主要光合特性和叶绿素荧光参数对不同干旱胁迫的响应。结果表明：轻度干旱胁迫时，棉花幼苗最大光化学效率（Fv/Fm）、PSII实际光化学效率（ΦPSII）、光化学猝灭系数（qP）、中短期干旱的净光合速率（Pn）、气孔导度（Gs）、蒸腾速率（Tr）随胁迫时间的延长上升，非光化学猝灭系数（qN）随胁迫时间的延长下降，但只有胁迫5 d的Fv/Fm与15 d 的Gs、胞间CO2浓度（Ci）、Tr在不同基因型间有显著差异；中度胁迫时，4个品种的ΦPSII、Pn、Gs和Tr随胁迫时间的延长略呈上升趋势，胁迫10 d 的Gs和15 d的Gs、Ci、Tr在不同基因型间显著差异；重度胁迫时，棉花幼苗Fv/Fm随胁迫时间的延长轻微上升，而ΦPSII、qP、qN、Pn和Gs呈上升-下降的变化趋势，Ci和Tr呈下降-上升-下降的趋势，且胁迫5 d的Fv/Fm，15 d的Fv/Fm、qN，10 d的Gs、Ci以及15 d的Pn、Gs、Ci、Tr均在不同基因型间有显著差异。从总体看，耐旱型棉苗的Fv/Fm高于干旱敏感型棉苗，耐旱棉苗的qN低于干旱敏感型棉苗，耐旱型棉苗的光合参数均低于干旱敏感型棉苗。建议苗期大田棉花耐旱性鉴定的叶绿素荧光辅助方法为：胁迫程度重度，处理时长15 d，参数为Fv/Fm和qN；光合特性辅助方法为：胁迫程度重度，处理时长15 d，参数为Pn、Gs、Ci、Tr。
Abstract:
In order to establish on assistant identification method for chlorophyll fluorescence parameters and photosynthesis characteristics of drought-tolerant cotton, response of photosynthesis and chlorophyll fluorescence of cotton seedlings to drought stress was studied using four cotton varieties with different drought-tolerant genotype under field experiment in northern Xinjiang. The results showed that the maximal photochemical efficiency(Fv/Fm), the actual photochemical efficiency of PSII (ΦPSII), photochemical quenching(qP), net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (Tr) increased with the prolongaction of drought stress time, but non-photochemical quenching (qN) decreased. There were significant differences between Fv/Fm value measured at the fifth day and Gs, intercellular CO2 concentration (Ci), Tr measured at the fifteenth day in cotton with different genotypes under mild drought stress. Under medium drought stress, the ΦPSII, Pn, Gs and Tr of all seedlings increased slightly with the prolongation of stress time, there were significant difference between the Gs at the tenth day and the Gs, Ci, Tr at the fifteenth day in cotton with different genotypes. If treated with severe stress, the Fv/Fm showed slight rising trend, but the change trends of the ΦPSII, qP, qN, Pn and Gs were rising-declining, and the trends of Ci and Tr were declining-rising-declining. There were significant differences among Fv/Fm at the fifth and fifteenth day, the qN at the fifteenth day, the Gs and Ci at the tenth and fifteenth day, the Pn and Tr at the fifteenth day in cotton with different genotype. Regardless of the intensity of stress, the Fv/Fm value of drought- tolerant cotton seedlings was higher than that of drought-sensitive cotton seedlings. The qN value of drought-tolerant cotton seedlings was lower than that of drought-sensitive cotton seedlings, and photosynthetic parameters of drought-tolerant cotton seedlings were lower than those of drought-sensitive cotton seedlings. The recommended assistant identification methods of chlorophyll fluorescence were intensity of stress: servere, stress time:15 days, chlorophyll fluorescence parameters: Fv/Fm and qN. The recommended assistant identification system of photosynthesis were intensity of stress: servere, stress time:15 days, chlorophyll fluorescence parameters: Pn, Gs, Ci, Tr.

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备注/Memo

备注/Memo:
收稿日期：2018-04-18 基金项目：国家自然科学基金项目（31360265、30800695）；石河子大学新品种培育专项（YZZX201602） 作者简介：徐建伟（1976-），男，甘肃临夏人，硕士，讲师，主要从事作物遗传育种及教学管理工作，(E-mail)438120660@qq.com 通讯作者：李志博，(E-mail)lzb_oea@shzu.edu.cn
更新日期/Last Update: 2019-02-27