[1]赵君,刘剑光,吴巧娟,等.棉花种质种仁含油量测定及其遗传多样性分析[J].江苏农业学报,2015,(05):975-983.[doi:doi:10.3969/j.issn.1000-4440.2015.05.006]
 ZHAO Jun,LIU Jian-guang,WU Qiao-juan,et al.Kernel oil content and genetic diversity of upland cotton germplasm[J].,2015,(05):975-983.[doi:doi:10.3969/j.issn.1000-4440.2015.05.006]
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棉花种质种仁含油量测定及其遗传多样性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年05期
页码:
975-983
栏目:
遗传育种·生理生化
出版日期:
2015-10-31

文章信息/Info

Title:
Kernel oil content and genetic diversity of upland cotton germplasm
作者:
赵君刘剑光吴巧娟赵亮许剑文肖松华
(江苏省农业科学院经济作物研究所/农业部长江下游棉花油菜重点实验室,江苏南京210014)
Author(s):
ZHAO JunLIU Jian-guangWU Qiao-juanZHAO LiangXU Jian-wenXIAO Song-hua
(Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed, Ministry of Agriculture, Nanjing 210014, China)
关键词:
棉花含油量遗传多样性关联分析
Keywords:
cottonoil contentgenetic diversityassociation analysis
分类号:
S634.3
DOI:
doi:10.3969/j.issn.1000-4440.2015.05.006
文献标志码:
A
摘要:
棉花是重要的经济作物,提高棉子的含油量具有重要的经济效益和社会意义。本研究以24份高油和低酚棉花品种(系)为材料,测定种仁的含油量及脂肪酸组分。结果显示,近期育成的棉花品种(系)种仁含油量显著低于高油材料的含油量,脂肪酸组分在不同品种(系)间存在显著差异,但与含油量之间没有显著的相关性。利用均匀分布于棉花26条染色体上的370对SSR标记对32个棉花品种(系)进行遗传多样性分析。共获得190个多态性位点,检测到609个等位变异,各位点检测到的等位变异数变幅为2~9个,平均为3.09个,多态信息含量(PIC)变幅为 0.07~0.81,平均为0.40。聚类分析将32份材料分为5个类群,其中12份高含油量种质材料划分成3个类群,剩余的20份种质材料划分成2个类群。关联分析检测到与种仁含油量性状显著关联的位点11个,分布在9条染色体上,用两种方法计算结果显示其解释表型变异为 0.043 6~0.144 9。
Abstract:
Cotton is an important economic crop. It has great economic and social benefits to improve oil content of cotton seeds.In this study, seed oil content and fatty acid composition of 24 cotton varieties were measured. The results showed that the oil contents of recently released cotton varieties were significantly lower than high oil materials, and fatty acid composition varied among different varieties. There was no correlation between fatty acid and oil content. 370 pairs of cotton SSR primers distributed on 26 chromosomes of cotton were used for the diversity analysis of 32 cotton varieties. 190 polymorphic loci and 609 alleles were detected. The allele number was 2-9 for these primers, with the average of 3.09, and polymorphic information content was about 0.07-0.81, with the average of 0.40. 32 varieties were clustered into five sub-branches, among which, all high oil materials were grouped in three sub-branches and the remaining 20 varieties in other two. By association analysis, 11 loci related to oil content traits were detected on nine chromosomes of cotton, which explained 0.043 6-0.144 9 of the phenotypic variation.〖JP〗

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备注/Memo

备注/Memo:
收稿日期:2015-07-16 基金项目:国家科技支撑计划项目(2013BAD01B03);863计划项目(2012AA101108);国家转基因重大专项(2014ZX08005);农业部保种项目(2015NWB039);江苏省科技支撑计划项目(BE2013380);江苏省农业科技自主创新基金项目[CX(13)2024] 作者简介:赵君(1982-),男,山西朔州人,博士,副研究员,研究方向为棉花种质创新与分子育种。(Tel) 025-84390363;(E-mail)sxzhaojun88@aliyun.com 通讯作者:肖松华,(E-mail)njxsh@sina.com
更新日期/Last Update: 2015-10-31