[1]杨柳,任春梅,缪倩,等.黄瓜绿斑驳花叶病毒的双抗夹心ELISA检测[J].江苏农业学报,2018,(04):769-774.[doi:doi:10.3969/j.issn.1000-4440.2018.04.008]
 YANG Liu,REN Chun-mei,MIAO Qian,et al.DAS-ELISA detection against cucumber green mottle mosaic virus[J].,2018,(04):769-774.[doi:doi:10.3969/j.issn.1000-4440.2018.04.008]
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黄瓜绿斑驳花叶病毒的双抗夹心ELISA检测()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2018年04期
页码:
769-774
栏目:
植物保护
出版日期:
2018-08-25

文章信息/Info

Title:
DAS-ELISA detection against cucumber green mottle mosaic virus
作者:
杨柳任春梅缪倩陆芳程兆榜
(江苏省农业科学院植物保护研究所,江苏南京210014)
Author(s):
YANG LiuREN Chun-meiMIAO QianLU FangCHENG Zhao-bang
(Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
黄瓜绿斑驳花叶病毒(CGMMV)单克隆抗体 双抗夹心ELISA
Keywords:
cucumber green mottle mosaic virus (CGMMV)monoclonal antibodyDAS-ELISA
分类号:
S41-30
DOI:
doi:10.3969/j.issn.1000-4440.2018.04.008
文献标志码:
A
摘要:
为建立快速、灵敏、准确且易操作的黄瓜绿斑驳花叶病毒(CGMMV)检测方法以实现早期诊断与控制病害,制备了8株效价大于 1×10-7的单克隆抗体,配对筛选结果表明经辣根过氧化物酶标记的A8H与单抗A3配对检测的灵敏度最高,并据此创制了双抗夹心ELISA试剂盒。该试剂盒对CGMMV具有特异反应,灵敏度达 1∶12 800(g∶ml),准确度与RT-PCR结果吻合,检测周期小于5 h,可用于生产上病害发生初期的快速诊断与鉴定。
Abstract:
To establish a cucumber green mottle mosaic virus (CGMMV) detection method which was rapid, sensitive, accurate and easy to operate for diagnosing and controlling the disease in the early stage, eight hybridoma cells lines were obtained, which could secrete anti CGMMV monoclonal antibody. The titer of the monoclonal antibody was more than 1×10-7. Monoclonal antibody A3 and A8H which labeled by horseradish peroxidase were used to establish DAS-ELISA kit because of their high sensitivity. The kit had a specific response to CGMMV and the accuracy was consistent with the RT-PCR results. The detection sensitivity of leaves was 1∶12 800 (g∶ml), and the whole process was within five hours. The DAS-ELISA detection could be used for rapid diagnosis at the early stage of production.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2017-12-14 基金项目:国家自然科学基金项目(31501610);公益性行业(农业)科研专项(201303028) 作者简介:杨柳(1984-),女,江苏徐州人,博士,助理研究员,研究方向为蔬菜病毒病。(E-mail)suiyangy@126.com 通讯作者:程兆榜,(E-mail)onlyone8501@126.com
更新日期/Last Update: 2018-09-04