[1]刘大同,邢潇悦,李东升,等.小麦穗分化不同阶段对低温胁迫的生理响应[J].江苏农业学报,2017,(06):1212-1219.[doi:doi:10.3969/j.issn.1000-4440.2017.06.003]
　LIU Da-tong,XING Xiao-yue,LI Dong-sheng,et al.The physiological response to low temperature of wheat during young spike development[J].,2017,(06):1212-1219.[doi:doi:10.3969/j.issn.1000-4440.2017.06.003]
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小麦穗分化不同阶段对低温胁迫的生理响应()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年06期
页码:
1212-1219
栏目:
遗传育种·生理生化
出版日期:
2017-12-30

文章信息/Info

Title:
The physiological response to low temperature of wheat during young spike development
作者:
刘大同1; 邢潇悦2; 李东升1; 胡文静1; 程晓明1; 高德荣1; 2; 程顺和1; 2
Author(s):
LIU Da-tong1; XING Xiao-yue2; LI Dong-sheng1; HU Wen-jing1; CHENG Xiao-ming1; GAO De-rong1; 2; CHENG Shun-he1; 2
(1.Institute of Agricultural Science of the Lixiahe District in Jiangsu Province/Key Laboratory of Wheat Biology and Genetic Improvement for Low and Middle Yangtze Valley, Ministry of Agriculture, Yangzhou 225007, China;2.Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China)
关键词:
小麦; 低温; 幼穗分化; 生理特性
Keywords:
wheat; low temperature; spike differentiation; physiological characteristics
分类号:
S512.1
DOI:
doi:10.3969/j.issn.1000-4440.2017.06.003
文献标志码:
A
摘要:
为明确不同冬春性小麦品种在相同穗分化进程下对低温的生理响应，选用冬性品种烟农19和春性品种宁麦4号、扬糯麦1号、宁麦13、扬麦11、镇10375，研究了-10 ℃人工低温胁迫下，护颖分化期、雌雄蕊分化期、药隔分化期和四分体形成期叶片中可溶性蛋白含量、丙二醛含量、超氧化物歧化酶、过氧化物酶和过氧化氢酶活性变化。结果表明，低温处理后，4个穗分化时期的可溶性蛋白含量和抗氧化酶活性均较0 h对照升高，但随着低温处理时间延长而下降；丙二醛含量呈先升后降再上升的趋势。药隔分化期对低温胁迫最为敏感。四分体形成期，叶片生理响应不宜作为评价抗寒性的指标。与0 h对照相比，烟农19的可溶性蛋白含量、超氧化物歧化酶、过氧化物酶和过氧化氢酶活性在低温处理后，上升幅度和响应速率大于其他5个春性品种，且丙二醛含量的增幅较小，对低温胁迫表现出较强抗性。
Abstract:
In order to define the physiological response of leaf to freezing temperature of different wheat cultivars during the same period of young spike development, the winterness cultivar Yannong 19 and springness cultivars Ningmai 4, Yangnuomai 1, Ningmai 13, Yangmai 11, Zhenmai 10375 were studied for the content of soluble protein and malondialdehyde (MDA), as well as the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), with the leaves treated under the low temperature stress of -10 ℃ during the glume differentiation stage, pistil and stamen differentiation stage, anther differentiation stage and the tetrad formation stage. The results showed that the soluble protein content and antioxidant activity during the four stages in the treatment of low temperature increased compared with the control, but decreased with the prolongation of the treatment. The content of MDA increased firstly, then decreased, and finally increased. Leaves of wheat in anther differentiation stage were most sensitive to low temperature stress. While the physiological response of leaves, which in the tetrad formation stage, should not be used to evaluate the cold resistance of wheat. Compared with the control, the soluble protein content, SOD, POD and CAT activities of Yannong 19 were higher than those of the other five cultivars after low temperature treatment, and the increase of MDA content was small. Thus the resistance to low temperature stress showed by Yannong 19 was the strongest in these six cultivars.

参考文献/References:

[1]王夏. 冬小麦低温灾害影响与诊断方法研究[D]. 北京：中国农业科学院, 2012: 1-3.
[2]姜丽娜,张黛静,宋飞,等. 不同品种小麦叶片对拔节期低温的生理响应及抗寒性评价[J]. 生态学报, 2014, 34 (15):4251-4261.
[3]王连臻，杜小凤，吴传万，等．小麦抗低温胁迫复配植物生长调节剂研发[J]．江苏农业科学，2015，43(3)：67-69.
[4]张志伟,王法宏,李升东,等. 不同类型小麦品种孕穗期低温生理反应极其抗寒性分析[J]. 麦类作物学报, 2012, 32(5):900-906.
[5]张自阳,冯素伟,茹振刚,等. 河南省主推小麦品种越冬期生理生化特性比较研究[J]. 河南科技学院学报, 2012, 40(4): 1-6.
[6]李玉刚，宫明波，王瑞英，等. 孕穗期低温对冬小麦穗部及籽粒的影响[J]．山东农业科学，2016，48（8）：55-59.
[7]张军,鲁敏,孙树贵,等. 拔节期低温胁迫对小麦生理生化特性和产量的影响[J]. 西北农业学报, 2014, 23(2): 73-79.
[8]皇甫自起,常守乾,李秀花,等. 小麦晚霜冻害的特点及预防补救技术[J]. 河南农业科学, 1996, 25(9): 6-9.
[9]李茂松,王道龙,张强, 等. 2004-2005年黄淮海地区冬小麦冻害成因分析[J]. 自然灾害学报, 2005, 24(4):51-55.
[10]郭天财,王永华. 小麦高产与防灾减灾技术[M]. 郑州：中原农民出版社, 2016: 66-68.
[11]孙苗苗,王志强,高翔,等. 河南主推小麦品种对低温胁迫的生理响应及耐寒性分析[J]. 麦类作物学报, 2016, 36 (3): 316-324.
[12]康国章,岳彩凤,彭慧芳,等. 冻害胁迫对小麦叶片抗寒生理生化指标的影响[J]. 河南农业科学, 2011, 40 (12): 56-60.
[13]SUSAN W, ATUL M, VAIDAYA G B, et al. High specific activity of whole cells in an aqueous organic two phase membrane bioreactor[J]. Enzyme and Microbial Technology, 1998, 22(7):575-577.
[14]DAT J, VANDENABEEL S, VRANOVA E, et al．Dual action of the active oxygen species during plant stress responses[J]．Cellular and Molecular Life Sciences, 2000, 57(5):779-795.
[15]潘晓云. 膜脂过氧化作为扁桃品种抗寒性鉴定指标研究[J]. 生态学报, 2002, 22(11):1902-1911.
[16]刘艳阳,李俊周,陈磊,等. 低温胁迫对小麦叶片细胞膜脂质过氧化产物及相关酶活性的影响[J]. 麦类作物学报, 2006, 26(4):70-73.
[17]胡新,黄绍华,黄建英. 晚霜冻害与小麦品种的关系—1998年霜冻害调查报告之一[J]. 中国农业气象, 1999, 20(3)：34-48.
[18]王春艳,李茂松,胡新,等. 黄淮地区冬小麦的抗晚霜冻害能力[J]. 自然灾害学报, 2006, 15(6):211-215.
[19]皇甫自起,常守乾,李秀花,等. 豫东地区小麦冻害调查分析[J]. 河南农业科学, 1996, 25 (4): 3-6.
[20]张志良. 植物生理学实验指导[M]. 北京：高等教育出版社, 1990:259-261
[21]王树刚,王振林,王平,等. 不同小麦品种对低温胁迫的反应及抗冻性评价[J]. 生态学报, 2011, 31(4): 1064-1072.
[22]潘杰,简令成. 植物寒害和抗寒机制中膜与蛋白质研究的进展[J]. 植物学通报, 1990, 7(1): 1-5.
[23]李春燕,徐雯,刘立伟,等. 药隔至开花期低温对小麦产量和生理特性的影响[J]. 麦类作物学报, 2016, 36(1): 77-85.
[24] PRASAD T K. Mechanism of chilling-induced oxidative stress injury and tolerance: changes in antioxidant system, oxidation of proteins and lipids and protease activities [J]. Plant Journal, 1996, 10(6):1017-1026.
[25]BEWLEY J D. Physiological aspects of desiccation tolerance[J]. Annual Review of Plant Physiology, 1979, 30(1): 195-238.
[26]孙大业. 植物细胞信号转导研究进展[J]. 植物生理学通讯， 1996, 32(2):81-91.
[27]陈贵,胡文玉,谢捕绨,等. 提取植物体内MDA的溶剂及MDA作为衰老指标的探讨[J].植物生理学通讯, 1991, 27(1): 44-46.
[28]PRASAD T K. Role of Catalase in inducing Chilling tolerance in pre-emergent maize Seedlings[J]. Plant Physiology, 1997, 114(4):1369-1376.

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备注/Memo

备注/Memo:
收稿日期：2017-07-25 基金项目：国家现代农业产业技术体系建设专项（CARS-3-1-1、CARS-3-2-11）；扬州市绿扬金凤人才计划项目（yzlyjfjh2015YB109）；江苏里下河地区农业科学研究所科研基金项目（lxh201504）；扬州市现代农业项目（YZ2016033） 作者简介：刘大同（1986-），男，山东宁阳人，博士，助理研究员，主要从事小麦的发育和生理研究。（Tel）15062799250；（E-mail）15062799250@163.com 通讯作者：高德荣，（E-mail）gdr@wheat.org.cn；程顺和，（E-mail）13905273926@126.com
更新日期/Last Update: 2018-01-03