[1]李晓霞,贾淼,金君华,等.原料乳中金黄色葡萄球菌环介导等温扩增(LAMP)检测方法的建立及应用[J].江苏农业学报,2017,(05):1171-1175.[doi:doi:10.3969/j.issn.1000-4440.2017.05.032]
 LI Xiao-xia,JIA Miao,JIN Jun-hua,et al.Establishment and application of loop-mediated isothermal amplification(LAMP) of Staphylococcus aureus in raw milk[J].,2017,(05):1171-1175.[doi:doi:10.3969/j.issn.1000-4440.2017.05.032]
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原料乳中金黄色葡萄球菌环介导等温扩增(LAMP)检测方法的建立及应用()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年05期
页码:
1171-1175
栏目:
加工贮藏·质量安全
出版日期:
2017-10-30

文章信息/Info

Title:
Establishment and application of loop-mediated isothermal amplification(LAMP) of Staphylococcus aureus in raw milk
作者:
李晓霞贾淼金君华张红星刘慧谢远红
(北京农学院食品科学与工程学院,农产品有害微生物及农残安全检测与控制北京市重点实验室/食品质量与安全北京实验室,北京102206)
Author(s):
LI Xiao-xiaJIA MiaoJIN Jun-huaZHANG Hong-xingLIU HuiXIE Yuan-hong
(College of Food Science and Engineering, Beijing Agricultural College/Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residues in Agricultural Products/Beijing Laboratory of Food Quality and Safety, Beijing 102206, China)
关键词:
环介导等温扩增(LAMP)金黄色葡萄球菌耐热核酸酶基因(nuc)灵敏度特异性
Keywords:
loop-mediated isothermal amplification(LAMP)Staphylococcus aureusthermostable nuclease genesensitivityspecificity
分类号:
TS207.4
DOI:
doi:10.3969/j.issn.1000-4440.2017.05.032
文献标志码:
A
摘要:
为了建立原料乳中金黄色葡萄球菌的环介导等温扩增(LAMP)检测方法,本研究根据金黄色葡萄球菌的耐热核酸酶基因(nuc)在保守区域设计特异性引物,建立LAMP检测方法,并用于原料乳中金黄色葡萄球菌的快速检测。结果表明,针对金黄色葡萄球菌的基因组DNA,使用设计合成的4条引物和建立的LAMP方法能够有效检测金黄色葡萄球菌DNA,每250 μl反应体系的灵敏度为1100 fg,并且此方法针对金黄色葡萄球菌具有良好的特异性。针对原料乳中金黄色葡萄球菌的污染,建立的LAMP检测方法的检测限为67 CFU/ml。本研究结果为进一步将LAMP方法应用于食品中金黄色葡萄球菌的安全检测奠定了基础。
Abstract:
The loop-mediated isothermal amplification(LAMP) method was established and applied in the rapid detection of Staphylococcus aureus in raw milk according to the conserved sequence of the heat-tolerant nuclease gene(nuc). The genomic DNA of S. aureus could be detected using the four primers designed and synthesized, and the detection limit was 1100 fg per 250 μl reaction system. The method was S. aureus specific, with the detection limit of 67 CFU/ml in raw milk. This study provided a basis for further application of LAMP method in the safety detection of S.aureus in food.

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备注/Memo

备注/Memo:
收稿日期:2017-04-25 基金项目:北京市长城学者培养计划项目(CIT&TCD20140315);北京农学院研究生改革与发展项目(2016YJS048) 作者简介:李晓霞(1990-),女,甘肃定西人,硕士研究生,主要从事食品生物检测研究。(Tel)15652319099;(E-mail)lixiaoxia0154 @163.com 通讯作者:谢远红,(Tel)010-80761345;(E-mail) xieyuanh@163.com
更新日期/Last Update: 2017-11-03