[1]周俊明,何孔旺,倪艳秀,等.铁离子限制下猪胸膜肺炎放线杆菌外膜囊泡的制备及其抗原性分析[J].江苏农业学报,2017,(01):119-123.[doi:10.3969/j.issn.1000-4440.2017.01.019 ]
 ZHOU Jun-ming,HE Kong-wang,NI Yan-xiu,et al.Preparation of out membrane vesicles released by Actinobacillus pleuropneumoniae grown under iron-restricted conditions and evaluation of their antigenicity in a murine model[J].,2017,(01):119-123.[doi:10.3969/j.issn.1000-4440.2017.01.019 ]
点击复制

铁离子限制下猪胸膜肺炎放线杆菌外膜囊泡的制备及其抗原性分析()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2017年01期
页码:
119-123
栏目:
畜牧兽医·水产养殖
出版日期:
2017-02-28

文章信息/Info

Title:
Preparation of out membrane vesicles released by Actinobacillus pleuropneumoniae grown under iron-restricted conditions and evaluation of their antigenicity in a murine model
作者:
周俊明何孔旺倪艳秀祝昊丹温立斌俞正玉茅爱华吕立新
(江苏省农业科学院兽医研究所,农业部兽用生物制品工程技术重点实验室,国家兽用生物制品工程技术研究中心,江苏 南京 210014)
Author(s):
ZHOU Jun-mingHE Kong-wangNI Yan-xiuZHU Hao-danWEN Li-binYU Zheng-yuMAO Ai-huaL Li-xin
(Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Engineering Research of Veterinary Bio-products, Ministry of Agriculture/ National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China)
关键词:
猪胸膜肺炎放线杆菌外膜囊泡IgA黏膜免疫
Keywords:
Actinobacillus pleuropneumoniaeouter membrane vesicleIgAmucosal immune
分类号:
S859.79+6
DOI:
10.3969/j.issn.1000-4440.2017.01.019
文献标志码:
A
摘要:
为评估猪胸膜肺炎放线杆菌外膜囊泡对小鼠免疫系统的刺激水平,以铁离子限制下体外培养猪胸膜肺炎放线杆菌shope菌株,经离心、0.22 μm过滤处理后获取无细胞培养上清,继而超速离心制备细菌释放的外膜囊泡(Outer membrane vesicles,OMV),以OMV作为亚单位疫苗2次鼻腔免疫小鼠。结果表明,通过透射电镜观察可见大部分OMV直径在50 nm至100 nm之间,OMV免疫小鼠长期增质量与PBS免疫组无显著差异,同时OMV不仅能够刺激小鼠血清产生高水平的IgG,而且在小鼠肺脏中产生高水平的IgA。可见,OMV是一种高效免疫刺激物,能有效地刺激小鼠肺脏黏膜产生免疫反应。
Abstract:
In order to investigate the contribution of outer membrane vesicles (OMV) to adaptive immune responses in mice, Actinobacillus pleuropneumoniae serotype 1(shope) was grown under iron restriction with respect to its immunogenic and protective potential. OMV released by shope were concentrated by ultracentrifugation and analyzed by electron microscopy, and OMVs were injected intranasally into Balb/c mice twice with 21 d intervals. The result showed the majority of OMVs’ size ranged from 50-100 nm. There was no significant differences between OMV immunized animals and the PBS treated group. The OMV induced a higher IgG response in serum and a higher IgA response in mice lungs. In conclusion, OMV was efficient immunostimulant and could stimulate mice lungs to produce mucosal immune.

参考文献/References:

[1]SEBUNYA T N, SAUNDERS J R. Haemophilus pleuropneumoniae infection in swine: a review [J]. J Am Vet Med Assoc, 1983, 182: 1331-1337.
[2]PILETTE C, OUADRHIRI Y, GODDING V,et al. Lung mucosal immunity: immunoglobulin-A revisited [J]. European Respiratory Journal, 2001, 18: 571-588.
[3]WOOF J M, KERR M A. The function of immunoglobulin A in immunity [J]. Journal of Pathology, 2006, 208: 270-282.
[4]JOLIE R A V, MULKS M H, THACKER B J. Cross-protection experiments in pigs vaccinated with actinobacillus-pleuropneumoniae subtypes 1a and 1b [J]. Vet Microbiol, 1995, 45: 383-391.
[5]FURESZ S E, MALLARD B A, BOSSE J T, et al. Antibody- and cell-mediated immune responses of Actinobacillus pleuropneumoniae-infected and bacterin-vaccinated pigs [J]. Infect Immun, 1997, 65: 358-365.
[6]VAN DEN BOSCH H, FREY J. Interference of outer membrane protein PalA with protective immunity against Actinobacillus pleuropneumoniae infections in vaccinated pigs [J]. Vaccine, 2003, 21: 3601-3607.
[7]HENSEL A, STOCKHOFEZURWIEDEN N, PETZOLDT K, et al. Oral Immunization of pigs with viable or inactivated Actinobacillus pleuropneumoniae serotype-9 induces pulmonary and systemic antibodies and protects against homologous aerosol challenge [J]. Infect Immun, 1995, 63: 3048-3053.
[8]FENWICK B, HENRY S. Porcine pleuropneumonia [J]. J Am Vet Med Assoc, 1994, 204: 1334-1340.
[9]O′HAGAN D T, VALIANTE N M. Recent advances in the discovery and delivery of vaccine adjuvants [J]. Nature Reviews Drug Discovery, 2003, 2: 727-735.
[10]KOSER M L, MCGETTIGAN J P, TAN G S, et al. Rabies virus nucleoprotein as a carrier for foreign antigens [J]. Proc Natl Acad Sci USA, 2004, 101: 9405-9410.
[11]SINGH M, CHAKRAPANI A, O′HAGON D. Nanoparticles and microparticles as vaccine-delivery systems [J]. Expert Review of Vaccines, 2007, 6: 797-808.
[12]GUY B. The perfect mix: recent progress in adjuvant research [J]. Nature Reviews Microbiology, 2007, 5: 505-517.
[13]JABBAL-GILL I, LIN W, KISTNER O,et al. Polymeric lamellar substrate particles for intranasal vaccination [J]. Advanced Drug Delivery Reviews, 2001, 51: 97-111.
[14]CHABALGOITY J A. Paving the way for the introduction of new vaccines into developing countries [J]. Expert Review of Vaccines, 2005, 4: 147-150.
[15]ELLIS T N, KUEHN M J. Virulence and immunomodulatory roles of bacterial outer membrane vesicles [J]. Microbiology and Molecular Biology Reviews, 2010, 74: 81-94.
[16]VAN DER POL L, STORK M, VAN DER LEY P. Outer membrane vesicles as platform vaccine technology [J]. Biotechnology Journal, 2015, 10: 1689-1706.
[17]O′RYAN M, STODDARD J, TONEATTO D, et al. A multicomponent meningococcal serogroup B vaccine (4CMenB): The clinical development program [J]. Drugs, 2014, 74: 15-30.
[18]GERLACH G F, ANDERSON C, POTTER A A, et al. Cloning and expression of a transferrin-binding protein from Actinobacillus pleuropneumoniae [J]. Infection and Immunity, 1992, 60: 892-898.
[19]BAUMGARTEN T, SPERLING S, SEIFERT J, et al. Membrane vesicle formation as a multiple-stress response mechanism enhances pseudomonas putida DOT-T1E Cell surface hydrophobicity and biofilm formation[J]. Applied and Environmental Microbiology, 2012, 17: 6217-6224. [20]BUETTNER F F R, KONZE S A, MAAS A, et al. Proteomic and immunoproteomic characterization of a DIVA subunit vaccine against Actinobacillus pleuropneumoniae [J]. Proteome Science, 2011, 9: 23.
[21]NEGRETE-ABASCAL E, GARCIA R M, REYES M E, et al. Membrane vesicles released by Actinobacillus pleuropneumoniae contain proteases and Apx toxins [J]. FEMS Microbiology Letters, 2000, 191: 109-113.
[22]GOTHE R, GONZLES O F, LINDNER T, et al. A novel strategy for protective Actinobacillus pleuropneumoniae subunit vaccines: detergent extraction of cultures induced by iron restriction [J]. Vaccine, 2001, 19: 966-975.
[23]BACHMANN M F, JENNINGS G T. Vaccine delivery: A matter of size, geometry, kinetics and molecular patterns [J]. Nature Reviews Immunology, 2010, 10: 787-796.
[24]ACEVEDO R, CALLIC A, ARANGUREN Y, et al. Immune adjuvant effect of V. cholerae O1 derived proteoliposome coadministered by intranasal route with Vi polysaccharide from Salmonella Typhi [J]. BMC Immunology, 2013, 14: 10.
[25]MURALINATH M, KUEHN M J, ROLAND K L, et al. Immunization with Salmonella enterica serovar typhimurium-derived outer membrane vesicles delivering the pneumococcal protein PspA confers protection against challenge with Streptococcus pneumoniae [J]. Infection and Immunity, 2011, 79: 887-894.

备注/Memo

备注/Memo:
收稿日期:2016-03-10 基金项目:公益性行业(农业)科研专项经费项目(201303034);江苏省自主创新探索性研究项目[CX(11)2060] 作者简介:周俊明(1983-),男,江苏东台人,硕士,副研究员,主要研究方向为动物细菌疫病防治。(Tel)025-84390988;(E-mail)zhoujm075@163.com
更新日期/Last Update: 2017-04-12