[1]张牧焓,张淼,孙冲,等.磷脂与Hsp90 相互作用的荧光和红外光谱[J].江苏农业学报,2016,(04):922-926.[doi:10.3969/j.issn.100-4440.2016.04.033]
 ZHANG Mu-han,ZHANG Miao,SUN Chong,et al.Interaction between phosphatidylcholine and heat shock protein 90 (Hsp90) by fluorescence and infrared spectroscopy[J].,2016,(04):922-926.[doi:10.3969/j.issn.100-4440.2016.04.033]
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磷脂与Hsp90 相互作用的荧光和红外光谱()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年04期
页码:
922-926
栏目:
加工贮藏·质量安全
出版日期:
2016-10-31

文章信息/Info

Title:
Interaction between phosphatidylcholine and heat shock protein 90 (Hsp90) by fluorescence and infrared spectroscopy
作者:
张牧焓1张淼12孙冲1王道营1卞欢1吴海虹1诸永志1耿志明1徐为民1
1.江苏省农业科学院农产品加工研究所,江苏 南京 210014;2.南京财经大学,食品科学与工程学院,江苏 南京 210046
Author(s):
ZHANG Mu-han1ZHANG Miao12SUN Chong1WANG Dao-ying1BIAN Huan1WU Hai-hong1ZHU Yong-zhi1GENG Zhi-ming1XU Wei-min1
1. Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2. College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing, 210046,China
关键词:
热休克蛋白90(Hsp90)磷脂酰胆碱荧光光谱红外光谱
Keywords:
heat shock protein 90 (Hsp90)phosphatidylcholinefluorescence spectroscopyinfrared spectroscopy
分类号:
TS251.5
DOI:
10.3969/j.issn.100-4440.2016.04.033
文献标志码:
A
摘要:
为研究磷脂酰胆碱(PC)与热休克蛋白90(Hsp90)的相互作用机制,分别采用荧光光谱和红外光谱法研究Hsp90内源荧光的变化和磷脂主要基团的变化。结果显示:PC对Hsp90内源荧光有较强的猝灭作用,PC对Hsp90的猝灭属于形成复合物产生的静态猝灭,结合位点数为0.92,结合的主要作用力为氢键和范德华力;Hsp90与PC的相互作用发生在磷脂极性头端和非极性侧链上,并使磷脂的结构发生改变。磷脂可以稳定地与Hsp90结合。
Abstract:
To investigate the mechanism underlying the interaction between phosphatidylcholine (PC) and heat shock protein 90 (Hsp90), the fluorescence spectroscopy and infrared spectroscopy were applied to study the changes of intrinsic fluorescence of Hsp90 and major groups of phosphatidylcholine respectively. The results showed PC was found to have a strong ability to quench the intrinsic fluorescence of Hsp90. It was a static quenching as the result of the formation of PC-Hsp90 complex. The number of binding sites was 0.92, and based on Van’t Hoff equation, the binding of PC to Hsp90 was driven mainly by hydrogen bond and Van der Waals forces. The infrared spectroscopy indicated the binding of Hsp90 and PC took place in the polar headgroup and nonpolar fatty acid side chains of PC, and the structure of PC molecules changed. In conclusion, PC could stably bind with Hsp90.

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-12-31基金项目:国家自然科学基金面上项目(31271891);国家自然科学基金青年基金项目(31401560);江苏省农业科技创新基金项目[CX(14)5058];江苏省农业科学院基本科研业务专项项目[ZX(15)4046]作者简介:张牧焓(1985-),女,江苏南京人,硕士,助理研究员,研究方向为肉品科学。(E-mail)zhangmh06@sina.com通讯作者:王道营,(E-mail)wdy0373@aliyun.com
更新日期/Last Update: 2016-11-01