[1]宝华宾,梁帅强,吕远大,等.玉米籽粒蛋白含量Meta-QTL及候选基因分析[J].江苏农业学报,2016,(04):736-745.[doi:10.3969/j.issn.100-4440.2016.04.004]
　BAO Hua-bin,LIANG Shuai-qiang,LYU Yuan- da,et al.Analysis of meta-QTL and candidate genes related to protein concentration in maize grain[J].,2016,(04):736-745.[doi:10.3969/j.issn.100-4440.2016.04.004]
点击复制

玉米籽粒蛋白含量Meta-QTL及候选基因分析()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年04期
页码:
736-745
栏目:
遗传育种·生理生化
出版日期:
2016-10-31

文章信息/Info

Title:
Analysis of meta-QTL and candidate genes related to protein concentration in maize grain
作者:
宝华宾1; 2; 梁帅强2; 吕远大2; 赵涵2
1. 南京农业大学农学院，江苏 南京 210095；2. 江苏省农业科学院农业生物技术研究所/江苏省农业生物学重点实验室，江苏 南京 210014
Author(s):
BAO Hua-bin1; 2; LIANG Shuai-qiang2; LYU Yuan-da2; ZHAO Han2
1.College of Agriculture, Nanjing Agricultural University, Nanjing 210095， China; 2.Provincial Key Laboratory of Agrobiology/Institute of Agricultural Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014， China
关键词:
玉米; 籽粒蛋白; 元分析; meta-QTL; 候选基因
Keywords:
maize;  grain protein content;  meta-analysis;  meta-QTL;  candidate gene
分类号:
S513.035.3
DOI:
10.3969/j.issn.100-4440.2016.04.004
文献标志码:
A
摘要:
玉米的蛋白含量与品质直接相关，可通过各种遗传群体对籽粒蛋白进行QTL分析。但是由于使用不同的遗传图谱和分子标记，结果之间无法直接比较分析，数据也无法整合和利用。为了综合、系统分析玉米籽粒蛋白的QTL位点，收集和整理了289个已报道的玉米籽粒蛋白含量QTL相关信息，采用BioMercator4.2软件整合不同遗传图谱，通过元分析发掘到44个meta-QTL（MQTL），研究确定了13个在不同遗传群体间具有一致性的QTL位点。利用生物信息学注释一致性QTL位点区段相关候选基因的功能，获得了847个候选基因，功能富集化分析发现13个候选基因显著富集在9个生物学过程中。这些meta-QTL位点及相关候选基因分析可以为玉米籽粒蛋白的分子标记辅助育种、精细定位和基因克隆提供有用的信息。
Abstract:
Although a variety of studies has been conducted for detecting the maize grain protein QTL with the different segregating populations and molecular markers, the consensus map and QTL are still unavailable which hampered the genetically pinpointing the loci responsible for phenotypic variations of protein concentrations.In this study, the information of 289 QTLs related to protein concentration in maize were collected from the published papers and were integrated into the reference map of IBM2 2008 Neighbors which is a high density genetic map. With meta-analysis method, 44 meta-QTL（MQTL）were detected. A total of 847 functional genes bracketed by the MQTL were predicted and annotated. Function annotation showed that the candidate genes were enriched significantly in nine biological processes. The analysis of meta-QTL and function enrichment will be beneficial for markerassisted selection breeding, fine mapping and gene isolation of grain protein concentration in maize.

参考文献/References:

[1]刘金龙，李波，王铁良，等． 沈阳地区滴施条件下施肥比例对玉米产量和品质的影响[J]． 江苏农业科学，2015，43(2): 95-98．
[2]柯永培, 石海春, 杨志荣. 玉米营养品质的遗传研究与育种进展 [J]. 玉米科学, 2004, 12(4):16-20.
[3]王敏杰. 玉米品质育种研究进展 [J]. 农业科技与装备, 2009(6): 1-3.
[4]GOLDMAN I L, ROCHEFORD T R, DUDLEY J W. Quantitative trait loci influencing protein and starch concentration in the Illinois Long Term Selection maize strains [J]. Theor Appl Genet, 1993, 87(1-2): 217-224.
[5]DUDLEY J W, CLARK D, ROCHEFORD T R, et al. Genetic analysis of corn kernel chemical composition in the random mated 7 generation of the cross of generations 70 of IHP×ILP [J]. Crop Science, 2007, 47(47): 45-57.
[6]DONG Y B, ZHANG Z W, SHI Q L, et al. QTL identification and metaanalysis for kernel composition traits across three generations in popcorn [J]. Euphytica, 2015, 204: 649-660.
[7]栗文娟, 刘志斋, 石云素, 等. 基于元分析和生物信息学分析的玉米抗旱相关性状 QTL 一致性区间定位 [J]. 作物学报, 2010, 36(9): 1457-1467.
[8]王帮太, 吴建宇, 丁俊强, 等. 玉米产量及产量相关性状 QTL 的图谱整合 [J]. 作物学报, 2009, 35(10): 1836-1843.
[9]江培顺, 张焕欣, 吕香玲, 等. 玉米产量相关性状 Meta-QTL 及候选基因分析 [J]. 作物学报, 2013, 39(6): 969-978.
[10] SOSNOWSKI O, CHARCOSSET A, JOETS J. BioMercator V3: an upgrade of genetic map compilation and QTL metaanalysis algorithms [J]. Bioinformatics, 2012, 28(15): 2082-2083.
[11] LV Y D, LIU Y H, ZHAO H. mInDel: a highthroughput and efficient pipeline for genomewide InDel marker development [J]. BMC Genomics, 2016, 17(1): 1-5.
[12] CONESA A, GOTZ S. Blast2GO:a comprehensive suite for functional analysis in plant genomics[J]. International Journal of Plant Genomics, 2008,2008:1-12.
[13] MELCHINGER A E, UTZ H. F, SCHN C C. Quantitative trait locus (QTL) mapping using different testers and independent population samples in maize reveals low power of QTL detection and large bias in estimates of QTL effects [J]. Genetics, 1998, 149(1): 383-403.
[14] WILLMOT D B, DUDLEY J W, ROCHEFORD T R, et al. Effect of random mating on markeroil associations for grain quality traits in the cross of Illinois high oil ×Illinois low oil [J]. Maydica, 2006, 51(2): 187-199.
[15] YANG G H, DONG Y B, LI Y L, et al. QTL verification of grain protein content and its correlation with oil content by using connected RIL populations of highoil maize [J]. Genetics and Molecular Research, 2014, 13 (1): 881-894.
[16] ZHANG J, LU X Q, SONG X F, et al. Mapping quantitative trait loci for oil, starch, and protein concentrations in grain with high-oil maize by SSR markers [J]. Euphytica, 2008, 162(3): 335-344.
[17] WASSOM J J, WONG J C, MARTINEZ E, et al. QTL associated with maize kernel oil, protein, and starch concentrations; kernel mass; and grain yield in Illinois high oil × B73 backcross-derived lines [J]. Crop Science, 2008, 48(1): 243-252.
[18] LI Y L, WANG Y Z, WEI M G, et al. QTL identification of grain protein concentration and its genetic correlation with starch concentration and grain weight using two populations in maize (Zea mays L.) [J]. Journal of Genetics, 2009, 88(1): 61-67.
[19] GUO Y Q, YANG X H, CHANDER S, et al. Identification of unconditional and conditional QTL for oil, protein and starch content in maize [J]. Crop Journal, 2013, 1(1): 34-42.
[20] 董永彬. 普通×爆裂玉米群体单性状及多性状联合QTL分析 [D]. 郑州: 河南农业大学, 2006.
[21] 冯晓曦. 玉米灌浆期不同阶段籽粒品质性状及灌浆速度的QTL分析 [D]. 郑州: 河南农业大学, 2008.
[22] 李学慧, 申顺先, 李玉玲, 等. 利用种子性状QTL定位高油玉米蛋白质含量QTL [J]. 作物杂志, 2011 (4): 40-42.
[23] 李玉玲, 王延召, 董永彬, 等. 利用利用三倍体胚乳遗传模型定位爆裂玉米子粒蛋白含量QTL [J]. 玉米科学, 2006, 14(6): 13-16.
[24] 石海春, 余学杰, 袁继超, 等. 玉米营养品质性状的QTL定位 [J]. 四川大学学报:自然科学版, 2009, 46(5): 1454-1460.
[25] 孙海艳, 蔡一林, 王久光, 等. 玉米籽粒主要品质的QTL定位 [J]. 农业生物技术学报, 2011, 19(4): 616-623.
[26] 孙海艳. 玉米籽粒主要品质的QTL定位 [D]. 重庆: 西南大学, 2008.
[27] 王延召. 玉米籽粒品质性状QTL定位及其遗传相关研究 [D]. 郑州: 河南农业大学, 2007.[28] 谢惠玲, 段柳静, 吴欣, 等. 不同发育时期玉米籽粒蛋白质含量的QTL分析 [J]. 河南农业大学学报, 2008, 42(4): 372-374.
[29] 杨国虎. 玉米两个相关RILs群体遗传图谱构建及主要性状QTL分析 [D]. 郑州: 河南农业大学, 2011.
[30] 杨美丽. 利用RILs群体定位玉米不同时期籽粒灌浆及品质性状QTL [D]. 郑州: 河南农业大学, 2010.
[30] 张淑贞. 玉米DH群体品质性状的分离及QTL分析 [D]. 保定: 河北农业大学, 2008.
[31] 张中伟. 普通×爆裂玉米RILs构建及主要性状QTL分析 [D]. 郑州: 河南农业大学, 2009.
[32] 张姿丽, 蒋锋, 刘鹏飞, 等. 鲜食甜玉米籽粒蛋白质含量的QTL定位 [J]. 西北农林科技大学:自然科学版, 2013, 41(3): 10-104.
[34] 赵素贞. 不同发育时期玉米籽粒品质性状的QTLs动态分析 [D]. 乌鲁木齐: 新疆农业大学, 2007.
[35] DONG J Q, FENG Y P, KUMAR D, et al. Analysis of tandem gene copies in maize chromosomal regions reconstructed from long sequence reads [J]. PNAS, 2016, 113(29): 7949-7956.[36] SONG R T, MESSING J. Gene expression of a gene family in maize based on noncollinear haplotypes [J]. PNAS, 2003, 100(15): 9055-9060.[37] ZHANG H W, UDDIN M S, ZOU C, et al. Meta-analysis and candidate gene mining of low-phosphorus tolerance in maize [J]. Journal of Integrative Plant Biology, 2014, 56(3): 262-270.[38] CHARDON F, VIRLON B, MOREAU L, et al. Genetic architecture of flowering time in maize as inferred from quantitative trait loci metaanalysis and synteny conservation with the rice genome [J]. Genetics, 2004, 168(4): 21692185.[39] HARTHILL J E, MEEK S E M, MORRICE N, et al. Phosphorylation and 14-3-3 binding of Arabidopsis trehalose-phosphate synthase 5 in response to 2-deoxyglucose [J]. Plant Journal, 2006, 47(2): 211-223.[40] ZHANG Y H, PRIMAVESI L F, JHURREEA D, et al. Inhibition of SNF1-related protein kinase1 activity and regulation of metabolic pathways by trehalose-6-phosphate [J]. Plant Physiology, 2009, 149(4): 186-1871.[41] SONG J N, JI C G, ZHANG J Z H. Insights on Na+-binding and conformational dynamics in multidrug and toxic compound extrusion transporter NorM [J]. Proteins, 2014, 82(2): 24-249.[42] MCHARDY A C, TAUCH A, RCKERT C, et al. Genome-based analysis of biosynthetic aminotransferase genes of corynebacterium glutamicum [J]. Journal of Biotechnology, 2003, 104(1-3): 229-240.[43] KRAKER J W D, LUCK K, TEXTOR S, et al. Two Arabidopsis genes (IPMS1 and IPMS2) encode isopropylmalate synthase, the branchpoint step in the biosynthesis of leucine [J]. Plant Physiology, 2007, 143(2): 97-986.[44] MCKEL B, EGGELING L, SAHM H. Functional and structural analyses of threonine dehydratase from corynebacterium glutamicum [J]. Journal of Bacteriology, 1992, 174(24): 8065-8072.[45] WU Y R, WANG W Q, MESSING J. Balancing of sulfur storage in maize seed [J]. BMC Plant Biology, 2012, 12: 77.[46] SAID J I, LIN Z X, ZHANG X L, et al. A comprehensive meta QTL analysis for fiber quality, yield, yield related and morphological traits, drought tolerance, and disease resistance in tetraploid cotton [J]. BMC Genomics, 2013, 14(1): 776.[47] WANG Y J, XU J, DENG D X, et al. A comprehensive metaanalysis of plant morphology, yield, staygreen,and virus disease resistance QTL in maize (Zea mays L.) [J]. Planta, 2016, 243(2): 459-471.[48] SALVI S, SPONZA G, MORGANTE M, et al. Conserved noncoding genomic sequences associated with a floweringtime quantitative trait locus in maize [J]. PNAS, 2007, 104(27): 11376-11381.[49] DUDLEY J W, LAMBERT R J. Ninety generations of selection for oil and protein in maize[J]. Maydica, 1992, 37: 1-7.[50] 魏良明, 戴景瑞, 刘占先, 等. 普通玉米蛋白质、淀粉和油分含量的遗传效应分析 [J]. 中国农业科学, 2008, 41(11): 3845-3850.

相似文献/References:

[1]印志同,秦秋霞,阚欣,等.玉米快速叶绿素荧光参数全基因组关联分析[J].江苏农业学报,2016,(04):746.[doi:10.3969/j.issn.100-4440.2016.04.005]
　YIN Zhi-tong,QIN Qiu-xia,KAN Xin,et al.Genome-wide association analysis of fast chlorophyll fluorescence parameters in maize[J].,2016,(04):746.[doi:10.3969/j.issn.100-4440.2016.04.005]
[2]岳海旺,陈淑萍,彭海成,等.玉米籽粒灌浆特性品种间比较[J].江苏农业学报,2016,(05):1043.[doi:10.3969/j.issn.1000-4440.2016.05.014]
　YUE Hai-wang,CHEN Shu-ping,PENG Hai-cheng,et al.Grain filling characteristics in maize materials[J].,2016,(04):1043.[doi:10.3969/j.issn.1000-4440.2016.05.014]
[3]周玲,梁帅强,林峰,等.玉米二态性 InDel 位点的鉴定和分子标记开发[J].江苏农业学报,2016,(06):1223.[doi:doi:10.3969/j.issn.1000-4440.2016.06.005]
　ZHOU Ling,LIANG Shuai-qiang,LIN Feng,et al.Biallelic InDel loci detection and molecular marker development in maize[J].,2016,(04):1223.[doi:doi:10.3969/j.issn.1000-4440.2016.06.005]
[4]刘朝茂,李成云.玉米与大豆间作对玉米叶片衰老的影响[J].江苏农业学报,2017,(02):322.[doi:doi:10.3969/j.issn.1000-4440.2017.02.013]
　LIU Chao-mao,LI Cheng-yun.Effects of maize/soybean intercropping on maize leaf senescence[J].,2017,(04):322.[doi:doi:10.3969/j.issn.1000-4440.2017.02.013]
[5]江彬,毕银丽,申慧慧,等.氮营养与AM真菌协同对玉米生长及土壤肥力的影响[J].江苏农业学报,2017,(02):327.[doi:doi:10.3969/j.issn.1000-4440.2017.02.014]
　JIANG Bin,BI Yin-li,SHEN Hui-hui,et al.Synergetic effects of Arbuscular mycorrhizal fungus and nitrogen on maize growth and soil fertility[J].,2017,(04):327.[doi:doi:10.3969/j.issn.1000-4440.2017.02.014]
[6]李国锋,葛敏,吕远大.Opaque2转录因子对玉米α-醇溶蛋白基因家族成员表达的影响[J].江苏农业学报,2015,(06):1224.[doi:doi:10.3969/j.issn.1000-4440.2015.06.006]
　LI Guo-feng,GE Min,L Yuan-da.Differential expression of α-zein family genes regulated by Opaque2 transcription factor[J].,2015,(04):1224.[doi:doi:10.3969/j.issn.1000-4440.2015.06.006]
[7]管莉,张阿英.CaM 与 ZmCCaMK 相互作用参与 BR 诱导的玉米叶片抗氧化防护[J].江苏农业学报,2015,(01):10.[doi:10.3969/j.issn.1000-4440.2015.01.002]
　GUAN Li,ZHANG A-ying.CaM-ZmCCaMK interaction involved in brassinosteroid-induced antioxidant defense in leaves of maize[J].,2015,(04):10.[doi:10.3969/j.issn.1000-4440.2015.01.002]
[8]王元琮,何冰,林峰,等.调控玉米阻止授粉后叶片衰老的QTL定位[J].江苏农业学报,2017,(04):747.[doi:doi:10.3969/j.issn.1000-4440.2017.04.004]
　WANG Yuan-cong,HE Bing,LIN Feng,et al.QTL mapping for pollination-prevention on leaf senescence[J].,2017,(04):747.[doi:doi:10.3969/j.issn.1000-4440.2017.04.004]
[9]田礼欣,李丽杰,刘旋,等.外源海藻糖对盐胁迫下玉米幼苗根系生长及生理特性的影响[J].江苏农业学报,2017,(04):754.[doi:doi:10.3969/j.issn.1000-4440.2017.04.005]
　TIAN Li-xin,LI Li-jie,LIU Xuan,et al.Root growth and physiological characteristics of salt-stressed maize seedlings in response to exogenous trehalose[J].,2017,(04):754.[doi:doi:10.3969/j.issn.1000-4440.2017.04.005]
[10]王尔美,李卫国,顾晓鹤,等.基于光谱特征分异的玉米种植面积提取[J].江苏农业学报,2017,(04):822.[doi:doi:10.3969/j.issn.1000-4440.2017.04.015]
　WANG Er-mei,LI Wei-guo,GU Xiao-he,et al.Planting area extraction of maize based on spectral features differentiation[J].,2017,(04):822.[doi:doi:10.3969/j.issn.1000-4440.2017.04.015]

备注/Memo

备注/Memo:
收稿日期：2016-01-31基金项目：国家自然科学基金项目（31401393）作者简介：宝华宾（1989-），男，河南焦作人,硕士研究生,主要从事玉米遗传育种研究。(E-mail)baohuabin_corns@163.com通讯作者：赵涵，(Tel)025-84390751；(E-mail)zhaohan@jaas.ac.cn
更新日期/Last Update: 2016-11-01