[1]易能,薛延丰,石志琦,等.微囊藻毒素对水稻种子萌发和幼苗生长的胁迫作用[J].江苏农业学报,2016,(04):729-735.[doi:10.3969/j.issn.100-4440.2016.04.003]
　YI Neng,XUE Yan-feng,SHI Zhi-qi,et al.Inhibitory effect of microcystins on seed germination and seedling growth of rice[J].,2016,(04):729-735.[doi:10.3969/j.issn.100-4440.2016.04.003]
点击复制

微囊藻毒素对水稻种子萌发和幼苗生长的胁迫作用()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年04期
页码:
729-735
栏目:
遗传育种·生理生化
出版日期:
2016-10-31

文章信息/Info

Title:
Inhibitory effect of microcystins on seed germination and seedling growth of rice
作者:
易能1; 薛延丰2; 石志琦2; 陈健2
1.江苏省农业科学院农业资源与环境研究所, 江苏 南京 210014; 2.江苏省农业科学院食品质量安全与检测研究所,江苏 南京 210014
Author(s):
YI Neng1; XUE Yan-feng2; SHI Zhi-qi2; CHEN Jian2
1.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2.Institute of Food Quality Safety and Detection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
关键词:
微囊藻毒素; 水稻; 淀粉酶; 氧化损伤; 生物富集系数
Keywords:
microcystin (MC-LR);  rice;  amylase;  oxidative injury;  bioconcentration factor
分类号:
X524
DOI:
10.3969/j.issn.100-4440.2016.04.003
文献标志码:
A
摘要:
为了明确微囊藻毒素污染的农用灌溉水对水稻生长的潜在威胁，研究了微囊藻毒素MC-LR对水稻种子萌发和水稻幼苗生长的影响。结果显示：低浓度MC-LR(0.05～1.00 μg/ml)对水稻种子萌发无显著影响，高浓度MC-LR (5.00 μg/ml) 则显著抑制种子萌发(P<0.05)；水稻种子淀粉酶活性随着MC-LR处理浓度的升高而逐渐降低(P<0.05)；随着MC-LR处理浓度的升高，MC-LR在水稻幼苗体内的累计量逐渐升高，但生物富集系数逐渐降低，水稻幼苗地下部分的MC-LR累积量高于地上部； MC-LR处理显著抑制水稻幼苗的生长(P<0.05)，且水稻幼苗的长势与其体内累积的MC-LR量呈显著负相关(P＜0.05)； 0.05μg/ml的MC-LR处理不影响水稻幼苗体内MDA含量和抗氧化酶 (SOD、CAT、POD) 的活性，而 0.10～5.00 μg/ml的MC-LR处理则导致上述生化指标的显著上升。表明,MC-LR胁迫能够抑制水稻种子萌发，并在水稻幼苗体内显著累积，诱导氧化损伤，抑制幼苗生长。
Abstract:
To investigate the potential risk of microcyntincontaminated water on the growth of rice, the effect of MC-LR on seed germination and seedling growth of rice was investigated. MC-LR at low concentration (0.05-1.00 μg/ml) exhibited no influence on the germination of rice seed while MC-LR at high concentration (5.00 μg/ml) significantly inhibited the germination (P<0.05). Treatment with MC-LR significantly inhibited the activity of amylase in rice seed in a dosedependent manner (P<0.05). The increase in MC-LR concentration resulted in the increase in MC-LR concentration in rice seedlings but the decrease in bioconcentration factor of MC-LR in rice seedlings. The roots accumulated more MC-LR than shoots. Treatment with MC-LR remarkably inhibited the growth of rice seedlings (P<0.05), and a significantly negative correlation was found between MC-LR concentration in seedlings and the growth of seedlings (P＜0.05). The oxidative indices, such as MDA concentration and the activities of antioxidative enzymes (SOD, CAT, and POD), were not affected by MC-LR at low concentration (0.05 μg/ml). However, MC-LR at 0.1-5.00 μg/ml significantly induced the increase in the oxidative indices. Taken together, MC-LR stress inhibited seed germination and seedling growth of rice by decreasing the activity of amylase in rice seed and inducing oxidative injury in rice seedling.

参考文献/References:

[1] DUAN H, MA R, XU X, et al. Twodecade reconstruction of algal blooms in China′s Lake Taihu[J].Environmental Science & Technology, 2009, 43:3522-3528.
[2]MAO X W, XU F, XU B,et al. Changes of water quality and eutrophication in Taihu Lake[J]. Water Resources Protection, 2009, 25:48-51.
[3]XIAO F G, ZHAO X L, TANG J, et al. Determination of microcystinLR in water from Lake Tai, China[J]. Bulletin of Environmental Contamination and Toxicology, 2009,82:23-233.
[4]CAMPOS A, VASCONCELOS V. Molecular mechanisms of microcystin toxicity in animal cells[J].International Journal of Molecular Sciences, 2010, 11:268-287.
[5]胡乐琴，吴春燕，杨秀文，等． 微囊藻毒素ELISA 检测方法的建立与评估[J]． 江苏农业科学，2015，43(9): 34-343．
[6]DITTMANN E, WIEGAND C. Cyanobacterial toxins occurrence, biosynthesis and impact on human affairs[J]. Molecular Nutrition & Food Research, 2006, 50:7-17.
[7]MOHAMED Z A, SHEHRI A M. Microcystins in groundwater wells and their accumulation in vegetable plants irrigated with contaminated waters in Saudi Arabia[J]. Journal of Hazardous Materials, 2009, 172:31-315.
[8]CHEN J, SONG L, DAI J,et al. Effects of microcystins on the growth and the activity of superoxide dismutase and peroxidase of rape (Brassica napus L.) and rice (Oryza sativa L.)[J].Toxicon, 2004, 43:393-400.
[9]易能,薛延丰,李慧明,等. 微囊藻毒素对油菜幼苗生长及抗氧化酶活性的影响[J]. 西北植物学报, 2009, 29(6):1207-1213.
[10] 李慧明,薛延丰,易能,等. 藻毒素对青菜生长的影响及其在幼苗体内的积累[J].江苏农业学报, 2009, 25(3):68-684.
[11] WANG Z, XIAO B, SONG L, et al. Effects of microcystinLR, linear alkyl benzene sulfonate and their mixture on lettuce (Lactuca sativa L.) seeds and seedlings[J].Ecotoxicology, 2011, 20:803-814.
[12] CRUSH J R, BRIGGS L R, SPROSEN J M, et al. Effect of irrigation with lake water containing microcystin on the content and growth of ryegrass, clover, rape, and lettuce[J].Environmental Toxicology, 2008, 23:246-252.
[13] PEUTHERT A, CHAKRABARTI S, PFLUGMACHER S. Uptake of microcystinsLR andLF (cyanobacterialtoxins) in seedlings of several important agricultural plant species and the correlation with cellular damage (lipid peroxidation)[J]. Environmental Toxicology, 2007, 22:436-442.
[14] JRVENPS, LUNDBERGNIINIST C, SPOOF L, et al. Effects of microcystins on broccoli and mustard, and analysis of accumulated toxin by liquidchromatographymass spectrometry[J]. Toxicon, 2007, 49:865-874.
[15] CHEN J, HU L B, ZHOU W, et al. Degradation of microcystinLR and RR by a Stenotrophomonas sp. strain EMS isolated from Lake Taihu, China[J]. International Journal of Molecular Sciences, 2010, 11: 896-911.
[16] ZHANG H, TAN Z Q, HU L Y, et al. Hydrogen sulfide alleviates aluminum toxicity in germinating wheat seedlings[J]. Journal of Integrative Plant Biology, 2010, 52 (6): 556-567.
[17] McCORD J M，FRIDOVICH I. Superoxide dismutase an enzymic function for erythrocuprein (hemocuprein)[J]. Journal of Biological Chemistry, 1969, 244(22):6049-6055.
[18] XUE Y F, LIU L, LIU Z P, et al. Protective role of Ca against NaCl toxicity in Jerusalem artichoke by upregulation of antioxidant enzymes[J]. Pedosphere, 2008, 18(6): 766-774.
[19] CHEN J, SHIYAB S, HAN F X, et al. Bioaccumulation and physiological effects of mercury in Pterisvittata and Nephrolepisexaltata[J]. Ecotoxicology, 2009, 18:11-121.
[20] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding[J].Analytical Biochemistry, 1976, 72:248-254.
[21] NURATA T, AKAZAWA T, FUKUCHI S. Enzymic mechanism of starch breakdown in germinating rice seeds I. an analytical study[J]. Plant Physiology, 1968, 43(12): 1899-1905.
[22] 王娓敏，邓玙，邹华，等. 微囊藻毒素对水稻根系生长和抗氧化系统的影响[J]. 环境科学, 2014(4):1468-1472.
[23] GILL S S, TUTEJA N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J]. Plant Physiology and Biochemistry,2010, 48: 909-930.
[24] 尹干，李慧明，陈健，等． 外源一氧化氮对微囊藻毒素诱导青菜氧化胁迫的缓解[J]． 江苏农业学报， 2015， 31(2): 253-259．
[25] AMADO L L, MONSERRAT J M. Oxidative stress generation by microcystins in aquatic animals: why and how[J]. Environmental International, 2010, 36: 226235.

相似文献/References:

[1]王士磊,丁正权,黄海祥.水稻隐性早熟突变体ref早熟性的遗传分析和基因定位[J].江苏农业学报,2016,(04):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
　WANG Shi-lei,DING Zheng-quan,HUANG Hai-xiang.Inheritance and gene mapping of recessive earliness in rice (Oryza sativa L.)[J].,2016,(04):721.[doi:10.3969/j.issn.100-4440.2016.04.001]
[2]王在满,郑乐,张明华,等.不同播种方式对直播水稻倒伏指数和根系生长的影响[J].江苏农业学报,2016,(04):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
　WANG Zai-man,ZHENG Le,ZHANG Ming-hua,et al.Effects of seeding manners on lodging index and root growth of directseeded rice[J].,2016,(04):725.[doi:10.3969/j.issn.100-4440.2016.04.002]
[3]刘凯,王爱民,严国红,等.一个水稻显性矮秆突变体的遗传特性与降株高能力[J].江苏农业学报,2016,(05):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
　LIU Kai,WANG Ai-min,YAN Guo-hong,et al.Genetic analysis and plant height reduction of a dominant dwarf mutant of rice[J].,2016,(04):968.[doi:10.3969/j.issn.1000-4440.2016.05.002]
[4]王红,杨镇,裴文琪,等.功能性微生物制剂对镉胁迫下水稻生长及生理特性的影响[J].江苏农业学报,2016,(05):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
　WANG Hong,YANG Zhen,PEI Wen-qi,et al.Growth and physiological characteristics of cadmium-stressed rice influenced by functional microorganism agent[J].,2016,(04):974.[doi:10.3969/j.issn.1000-4440.2016.05.003]
[5]孙玲,单捷,毛良君,等.基于遥感和Moran's I指数的水稻面积变化空间自相关性研究[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
　SUN Ling,SHAN Jie,MAO Liang-jun,et al.Spatial autocorrelation of changes in paddy rice area based on remote sensing and Moran’s I index[J].,2016,(04):1060.[doi:10.3969/j.issn.1000-4440.2016.05.017]
[6]张晓忆,李卫国,景元书,等.多种光谱指标构建决策树的水稻种植面积提取[J].江苏农业学报,2016,(05):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
　ZHANG Xiao-yi,LI Wei-guo,JING Yuan-shu,et al.Extraction of paddy rice area by constructing the decision tree with multiple spectral indices[J].,2016,(04):1060.[doi:10.3969/j.issn.1000-4440.2016.05.018]
[7]裔传灯,李玮,王德荣,等.水稻GW5基因的1212-bp Indel变异对粒形的影响[J].江苏农业学报,2016,(06):1201.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
　YI Chuan-deng,LI Wei,WANG De-rong,et al.Effect of 1212-bp Indel variation of gene GW5 on rice grain shape[J].,2016,(04):1201.[doi:doi:10.3969/j.issn.1000-4440.2016.06.001]
[8]刘红江,陈虞雯,张岳芳,等.不同播栽方式对水稻叶片光合特性及产量的影响[J].江苏农业学报,2016,(06):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
　LIU Hong-jiang,CHEN Yu-wen,ZHANG Yue-fang,et al.Effects of planting pattern on leaf photosynthetic characteristics and yield of rice[J].,2016,(04):1206.[doi:doi:10.3969/j.issn.1000-4440.2016.06.002]
[9]郭保卫,许轲,魏海燕,等.钵苗机插水稻茎秆的抗倒伏能力[J].江苏农业学报,2016,(06):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]
　GUO Bao-wei,XU Ke,WEI Hai-yan,et al.Culm lodging resistance characteristics of bowl seedling mechanical-transplanting rice[J].,2016,(04):1280.[doi:doi:10.3969/j.issn.1000-4440.2016.06.014]
[10]董海霞,赵明柳,唐守寅,等.石灰对土壤中Cd 和Zn 形态及对水稻有效性的影响[J].江苏农业学报,2016,(06):1320.[doi:doi:10.3969/j.issn.1000-4440.2016.06.020]
　DONG Hai-xia,ZHAO Ming-liu,TANG Shou-yin,et al.The effects of liming on the fraction and bioavailability to rice of Cd and Zn in a contaminated soil[J].,2016,(04):1320.[doi:doi:10.3969/j.issn.1000-4440.2016.06.020]

备注/Memo

备注/Memo:
收稿日期：2015-11-10基金项目：国家自然科学基金项目（21207054）；江苏省农业科技自主创新基金项目[CX(13)5052]作者简介：易能(1984-)，女，湖南邵阳人，博士，副研究员，主要从事水体面源污染治理的基础研究。（E-mail）yineng5288ys@163.com通讯作者：陈健，（E-mail）dreamchenjian@163.com
更新日期/Last Update: 2016-11-01