[1]刘青涛,李 银,黄欣梅,等.坦布苏病毒对小鼠的神经致病力[J].江苏农业学报,2016,(03):619.[doi:10.3969/j.issn.1000-4440.2016.03.021]
 LIU Qing-tao,LI Yin,HUANG Xin-mei,et al.Neuropathogenicity of Tembusu virus in mice[J].,2016,(03):619.[doi:10.3969/j.issn.1000-4440.2016.03.021]
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坦布苏病毒对小鼠的神经致病力()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
619
栏目:
畜牧兽医·水产养殖
出版日期:
2016-06-30

文章信息/Info

Title:
Neuropathogenicity of Tembusu virus in mice
作者:
刘青涛 李 银 黄欣梅 韩凯凯 赵冬敏 刘宇卓 杨 婧
江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心,江苏 南京 210014
Author(s):
LIU Qing-tao LI Yin HUANG Xin-mei HAN Kai-kai ZHAO Dong-min LIU Yu-zhuo YANG Jing
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Animal Disease Diagnostic and Immunology/ Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio-products, Nanjing 210014, China
关键词:
坦布苏病毒 小鼠 神经致病力
Keywords:
Tembusu virus mouse neuropathogenicity
分类号:
S852.65+9.6
DOI:
10.3969/j.issn.1000-4440.2016.03.021
文献标志码:
A
摘要:
为评价坦布苏病毒(TMUV)对哺乳动物和人类健康的潜在危害,测定TMUV的增殖能力、神经毒力和神经侵袭力,并对小鼠脑组织进行病理学观察和炎性细胞因子测定,研究TMUV对小鼠的神经致病力。结果显示,TMUV可以在小鼠的脑内进行有效的复制; 3日龄和3周龄的小鼠经脑内注射TMUV后,可以导致小鼠的发病和死亡,其半致死剂量分别为1×100.5 ELD50和1×102.5 ELD50; 3周龄的小鼠经腹腔注射TMUV后,同样可以引起小鼠的死亡,并且可以在脑内检测到病毒; 此外,TMUV还在小鼠脑内引起了神经原的变性、炎性细胞的侵润和过度的炎性细胞因子应答。以上结果说明,TMUV可以在小鼠脑内进行有效复制并导致强烈的炎性应答,对小鼠具有较强的神经毒力和侵袭力。因此应该加强TMUV的流行病学监测和防控,防止该病毒的跨种间传播。
Abstract:
To evaluate the potential threat to mammals and human health caused by Tembusu virus(TMUV), the neuropathogenicity of TMUV to mice was detected by evaluation of the replication, neurovirulence and neuroinvasiveness of the virus, the brain histopathology and inflammatory cytokine responses. TMUV could replicate in mouse brain and showed half mouse lethal dose(MLD50)of 1×100.5 50% egg lethal doses(ELD50)and 1×102.5 ELD50 by intracerebral inoculation in 3-day- and 3-week-old mice, respectively. TMUV was also able to cause 3-week-old mice death and spread to mouse brain by intraperitoneally inoculation. In addition, neuronal degeneration, inflammatory cell infiltration,and high levels of infiammatory cytokine responses were induced in mice brains infected with TMUV. Taken together, TMUV could replicate and result in elevated inflammatory response in mouse brain, which led to strong neurovirulence and neuroinvasiveness in mice. It is necessary to improve the control of TMUV to prevent interspecies transmission.

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备注/Memo

备注/Memo:
收稿日期:2016-01-13
基金项目:中国博士后科学基金项目(2015M571714),江苏省博士后科研资助计划项目(1402025B),江苏省农业科技自主创新基金项目[CX(14)2091]
作者简介::刘青涛(1981-), 男,山东滨州人,博士,助理研究员,主要从事分子病毒学与免疫学研究。(Tel)025-84390047;(E-mail)taoqingliu2013@163.com
通讯作者:李 银,(Tel)025-84391687;(Email)muziyin08@163.com
更新日期/Last Update: 2016-06-30