[1]吴 双,朱善元,王晓泉,等.线粒体蛋白质翻译延伸因子(TUFM)在不同毒力新城疫病毒感染的鸡胚成纤维细胞中的表达水平[J].江苏农业学报,2016,(03):615.[doi:10.3969/j.issn.1000-4440.2016.03.020]
　WU Shuang,ZHU Shan-yuan,WANG Xiao-quan,et al.Expression level of mitochondrial Tu translation elongation factor(TUFM)in chicken embryonic fibroblasts(CEFs)infected with different virulent Newcastle disease viruses[J].,2016,(03):615.[doi:10.3969/j.issn.1000-4440.2016.03.020]
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线粒体蛋白质翻译延伸因子(TUFM)在不同毒力新城疫病毒感染的鸡胚成纤维细胞中的表达水平()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2016年03期
页码:
615
栏目:
畜牧兽医·水产养殖
出版日期:
2016-06-30

文章信息/Info

Title:
Expression level of mitochondrial Tu translation elongation factor(TUFM)in chicken embryonic fibroblasts(CEFs)infected with different virulent Newcastle disease viruses
作者:
吴 双1;  朱善元1;  王晓泉2;  左伟勇1;  王安平1;  王永娟1;  秦 枫1;  洪伟鸣1; 刘秀梵2
1.江苏农牧科技职业学院/江苏省兽用生物制药高技术研究重点实验室,江苏 泰州 225300; 2.扬州大学农业部畜禽传染病学重点开放实验室,江苏 扬州 225009
Author(s):
WU Shuang1;  ZHU Shan-yuan 1;  WANG Xiao-quan2;  ZUO Wei-yong1;  WANG An-ping1;  WANG Yong-juan1; QIN Feng1;  HONG Wei-ming1;  LIU Xiu-fan2
1. Jiangsu Agri-animal Husbandry Vocational College/Jiangsu Provincial Veterinary Bio-pharmaceutical, Key Laboratory of High-tech Research, Taizhou 225300, China; 2. Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China
关键词:
线粒体蛋白质翻译延伸因子Tu;  新城疫病毒;  表达水平;  毒力
Keywords:
mitochondrial Tu translation elongation factor;  Newcastle disease virus;  expression level;  virulence
分类号:
S858.31
DOI:
10.3969/j.issn.1000-4440.2016.03.020
文献标志码:
A
摘要:
为研究线粒体蛋白质翻译延伸因子Tu(TUFM)在不同毒力新城疫病毒(NDV)感染的鸡胚成纤维细胞(CEF)中表达水平的变化,运用比较蛋白质组技术分析感染了NDV的CEF差异表达的蛋白点。与24 h的阴性对照组相比,感染NDV后24 h时TUFM表达量在毒力最弱的WX-10-07-Pi感染组中显著升高; 毒力最强的JS-5-05-Go引起宿主表达TUFM量随感染时间增加逐渐增强。推测TUFM的表达水平与NDV毒力强弱具有一定相关性。
Abstract:
To study the dynamic expression of mitochondrial Tu translation elongation factor(TUFM)in chicken embryo fibroblasts(CEFs),comparative proteomics technique was used to analyze proteomes of CEFs infected with three different virulent Newcastle disease viruses(NDVs)derived from chicken, goose and pigeon. Mass spectrometry results showed the expression level of TUFM was upregulated in the low virulent strain WX-10-07-Pi-infected group compared with negative control group at 24 h post infection. The expression level of TUFM increased over time in the CEFs infected with high virulent strain JS-5-05-Go. These results suggest that the expression level of TUFM has a great deal of correlation with the virulence of NDVs.

参考文献/References:

[1] HIROAKI S, TAKUYA U, HIDEKI T, et al. Chaperone properties of mammalian mitochondrial translation elongation factor Tu [J]. Journal of Biological Chemistry, 2007, 282(6):4076-4084.
[2] 何成武. TUFM在膀胱癌中的表达及对T24细胞生物特性的影响[D]. 广州:南方医科大学, 2014.
[3] 詹 媛. 新城疫病毒通过激活真核翻译起始因子4F促进病毒增殖[D]. 北京:中国农业科学院, 2014.
[4] 詹 媛, 仇旭升, 曲昱蓉, 等. 新城疫病毒在感染早期通过Akt/mTOR/4E-BP1和p38/Erk/Mnk1通路激活宿主真核翻译起始因子eIF4E的研究[J]. 中国预防兽医学报, 2015, 37(1):1-5.
[5] 吴永平. 传染性法氏囊病毒感染鸡靶器官的比较蛋白质组研究[D]. 杭州:浙江大学, 2009.
[6] 吴 双, 开 妍, 王晓泉, 等. 新城疫病毒感染细胞的双向凝胶电泳方法建立及其初步分析[J]. 中国家禽, 2010, 32(10):25-29.
[7] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantifies of protein utilizing the principle of protein-dye binding [J]. Anal Biochem, 1976, 72:248-54.
[8] CANDIANO G, BRUSCHI M, MUSANTE L, et al. Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis [J]. Electrophoresis, 2004, 25:1327-1333.
[9] RIIS B, RATTAN S I S, CLARK B F C, et al. Eukaryotic protein elongation factors [J]. Trends in Biochemical Sciences, 1990, 15(11):420-424.
[10] NAGAO A, SUZUKI T, SUZUKI T. Aminoacyl-tRNA surveillance by EF-Tu in mammalian mitochondria [J]. Nucleic Acids Symp Ser(Oxf), 2007(51):41-42.
[11] PANTUA H D, MCGINNES L W, PEEPLES M E, et al. Requirements for the assembly and release of Newcastle disease virus-like particles [J]. J Virol, 2006, 80(22):11062-11073.
[12] 吴 双. 2005-2008年华东地区NDV遗传进化分析和代表性毒株感染细胞的比较蛋白质组研究[D]. 扬州:扬州大学, 2010.

备注/Memo

备注/Memo:
收稿日期:2015-09-30
基金项目:国家自然科学基金项目(31440083); 江苏省科技支撑计划项目(BE2012366); 江苏农牧科技职业学院科研项目(NSFPT201501)
作者简介::吴 双(1983-), 女, 江苏徐州人, 博士, 副教授, 主要从事动物传染病防控和兽用基因工程疫苗研究。(Tel)0523-86356818;(E-mail)jswushuang@sina.com.cn
通讯作者:刘秀梵,(Tel)0514-87991416;(E-mail)xfliu@yzu.edu.cn
更新日期/Last Update: 2016-06-30