[1]胡雪丹,张曼,侯茜,等.葫芦小孢子发育时期与花器形态相关性[J].江苏农业学报,2015,(06):1407-1412.[doi:doi:10.3969/j.issn.1000-4440.2015.06.033]
 HU Xue-dan,ZHANG Man,HOU Qian,et al.Relationship between microspore development period and flower organ morphology of bottle gourd (Lagenaria siceraria)[J].,2015,(06):1407-1412.[doi:doi:10.3969/j.issn.1000-4440.2015.06.033]
点击复制

葫芦小孢子发育时期与花器形态相关性()
分享到:

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1407-1412
栏目:
园  艺
出版日期:
2015-12-31

文章信息/Info

Title:
Relationship between microspore development period and flower organ morphology of bottle gourd (Lagenaria siceraria)
作者:
胡雪丹12张曼1侯茜1徐锦华1刘广1姚协丰1李苹芳1陈学好2羊杏平1
(1.江苏省农业科学院蔬菜研究所,江苏南京210014;2.扬州大学园艺与植物保护学院,江苏扬州225000)
Author(s):
HU Xue-dan12ZHANG Man1HOU Qian1XU Jin-hua1LIU Guang1YAO Xie-feng1LI Ping-fang1CHEN Xue-hao2YANG Xing-ping1
(1.Institute of Vegetable,Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;2.College of Horticulture and Plant Protection,Yangzhou 
关键词:
葫芦小孢子发育时期花器形态
Keywords:
gourdmicrosporedevelopmental stageflower organ morphology
分类号:
S642.9
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.033
文献标志码:
A
摘要:
为了能够快速准确判断葫芦小孢子发育时期,通过对葫芦花器外观形态与花药显微结构的观察,并将其与小孢子发育时期进行联系。结果显示:葫芦小孢子经过四分体时期、单核期和二核期后发育成成熟花粉粒;在顶芽生长点肉眼可辨别花芽和叶芽的1 d和2 d时,在 6.3~6.7 mm的花蕾中,小孢子多处于单核期,此时葫芦花雄蕊的花粉管干瘪较适合花药、小孢子培养;发育到第3 d、4 d时,花粉管较饱满,此时基本为发育成熟的花粉粒。表明可以根据葫芦花蕾的形态、大小及小孢子的发育时期,确定花药、小孢子培养最佳时期所对应的选蕾标准。
Abstract:
Researches on the relationship between the gourd microspore development and flower organ morphology were carried out to find a way to identify the microspore developmental period quickly and acccurately through observing bottle gourd flower appearance and anther microstructure. The results showed that bottle gourd microspore went through tetrad stage, uninucleate stage, binucleate stage, and finally developed to the mature pollen grain. In the growth of plant bud, the flower bud and leaf bud could be differentiated in the first one or two days . The bud size in 6.3-6.7 mm were mostly at the uninucleate stage of microspore. At this moment, the staminal tubes were shrivelled and suitable for anther and microspore culture. In the 3rd or 4th day,the tubes were full,and the microspore had basically grown into mature pollen grain. The develpoment period of gourd microspore could be determined by the morphology and size of the buds, which helps to define the standard of bud selection corresponding to the optimal stage of anther and microspore culture.

参考文献/References:

[1]朱德蔚,王德槟,李锡香. 中国作物及其野生近缘植物-蔬菜作物卷[M].北京:中国农业出版社,2008.
[2]SAVIN F,DECOMBLE V,LE COUVIOUR M,et al. The X-ray detection of haploid embryos arisen in muskmelon Cucumis melon L. seeds and resulting from a parthenogenetic development induced by irradiated pollen [J]. Cucurbit Genet Coop,1988,11:39-42.
[3]雷春,陈劲枫. 葫芦科蔬菜作物单倍体材料创制的研究进展[J].中国蔬菜,2006(1):33-36.
[4]詹艳,陈劲枫,AHMED A M. 黄瓜游离小孢子培养诱导成胚和植株再生[J].园艺学报,2009,6(2):221-226.
[5]王薇薇,羊杏平,范淑英,等. 甜瓜小孢子发育时期与花器形态关系研究[J].西北农林科技大学学报:自然科学版,2015,43(4):108-111.
[6]缑艳霞,张明方. 西瓜花药离体培养影响因子研究[J].北方园艺,2010(10):117-120.
[7]李娟. 西瓜花药和小孢子培养研究[D].成都:四川农业大学,2008.
[8]谢淼,秦丽颖,潘俊松,等. 黄瓜花器形态发生、小孢子发育与花药培养[J].西北植物学报,2005,25(6):1096-1100.
[9]张菊平,巩振辉,刘珂珂,等.辣椒小孢子发育时期与花器形态的相关性[J].西北农林大学学报:自然科学版,2007,35(3):153-158.
[10]林宗铿,张天翔,杨俊杰,等. 白芦笋小孢子发育时期与花器形态相关性研究[J].中国农学通报,2011,27(28):200-204.
[11]杨宏光,孙晓梅,王孝鹏,等. 三色堇小孢子发育时期与花器形态相关性分析[J].沈阳农业大学学报,2008,39(5):621-624.
[12]钱春桃,陈劲枫,娄群峰,等. 黄瓜花粉母细胞减数分裂行为的研究[J].武汉植物学研究,2003,21(3):193-197.
[13]任明迅. 植物雄蕊合生的多样性、适应意义及分类学意义初探[J].植物分类学报,2008,46(4):452-466.

相似文献/References:

[1]胡雪丹,张曼,侯茜,等.不同影响因子诱导葫芦花药愈伤组织[J].江苏农业学报,2016,(05):1155.[doi:10.3969/j.issn.1000-4440.2016.05.032]
 HU Xue-dan,ZHANG Man,HOU Qian,et al.Induction of calli in anther culture of gourd influenced by temperature, hormone and activated carbon[J].,2016,(06):1155.[doi:10.3969/j.issn.1000-4440.2016.05.032]

备注/Memo

备注/Memo:
收稿日期:2015-05-25 基金项目:国家西甜瓜产业技术体系项目(CARS-NO.8); 江苏省农业科技支撑计划项目(BE2012323) 作者简介:胡雪丹(1990-),女,江苏泰州人,硕士研究生,研究方向为葫芦游离小孢子培养。(E-mail) 276518157@qq.com 通讯作者:羊杏平,(E-mail)xingping@jaas.ac.cn
更新日期/Last Update: 2015-12-31