[1]王庆莲,吴伟民,赵密珍,等.无核葡萄及其杂交组合胚挽救技术[J].江苏农业学报,2015,(06):1413-1418.[doi:doi:10.3969/j.issn.1000-4440.2015.06.034]
　WANG Qing-lian,WU Wei-min,ZHAO Mi-zhen,et al.Embryo rescue techniques for seedless grapes and reciprocal cross combinations[J].,2015,(06):1413-1418.[doi:doi:10.3969/j.issn.1000-4440.2015.06.034]
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无核葡萄及其杂交组合胚挽救技术()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年06期
页码:
1413-1418
栏目:
园　　艺
出版日期:
2015-12-31

文章信息/Info

Title:
Embryo rescue techniques for seedless grapes and reciprocal cross combinations
作者:
王庆莲; 吴伟民; 赵密珍; 钱亚明; 夏瑾
（江苏省农业科学院园艺研究所/江苏省高效园艺作物遗传改良重点实验室，江苏南京210014）
Author(s):
WANG Qing-lian; WU Wei-min; ZHAO Mi-zhen; QIAN Ya-ming; XIA Jin
(Institute of Horticulture, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014,China)
关键词:
无核葡萄; 胚挽救; 培养基; 激素; 萌发
Keywords:
seedless grape; embryo rescue; medium; hormone; germination
分类号:
S663.1
DOI:
doi:10.3969/j.issn.1000-4440.2015.06.034
文献标志码:
A
摘要:
为研究无核葡萄及其杂交组合胚挽救技术的影响因素，以无核葡萄莫利莎无核、希姆劳特及其正反交杂交组合为试验材料，分析了培养基种类、6-BA浓度、胚珠处理方式和取样时期对无核葡萄胚珠发育和萌发的影响。结果显示，莫利莎无核葡萄胚挽救种胚萌发的最佳培养基及其组分为WPM培养基+6-BA 0.2 mg/L +水解酪蛋白质50.0 mg/L +活性炭3.0 g/L +琼脂6.5 g/L +蔗糖20.0 g/L；不同的胚珠处理均可以提高其萌发率，其中以横切处理为最佳；不同品种及其不同杂交组合胚挽救的最佳取样时期不同；母本对胚珠的萌发起着关键作用。表明在无核葡萄胚挽救技术育种实践中，应当根据不同的亲本及其杂交组合进行不同的优化研究。
Abstract:
To figure out the factors influencing embryo rescue in seedless grapes and their reciprocal cross combinations, the seedless grapes Melissa seedless, Himrod and their reciprocally crossed progenies were used as test materials to study the effects of basal medium varieties, 6-BA concentrations, ovule treating modes and sampling dates on the embryo development and germination. The results showed that the medium favoring the germination of ovules consisted of WPM, 6-BA 0.2 mg/L, LH 50.0 mg/L, activated carbon 3.0 g/L, agar 6.5 g/L, and sucrose 20.0 g/L. All the ovule treating modes improved their germination, among which, crosscut was the best. The best sampling dates for the embryo rescue varied among seedless grapes and their reciprocally crossed progenies. The female parent was the key to embryo germination. In the practice of embryo rescue, culture medium variety, 6-BA concentration, ovule treating mode and sampling date should be combined differently according to the grape parents and their cross combinations.

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备注/Memo

备注/Memo:
收稿日期：2015-06-03 基金项目：江苏省自然科学基金项目(BK20131331)；现代农业产业技术体系专项基金项目（CARS-3O） 作者简介：王庆莲（1979-），女，山东莱芜人，博士，副研究员，主要从事浆果类作物资源与遗传育种研究。（Tel）025-84390219;（E-mail）wang_qinglian@163.com 通讯作者：吴伟民，（E-mail）5wm@163.com
更新日期/Last Update: 2015-12-31