[1]叶幼荣,张璐,孙杰,等.蛋鸡子宫内膜细胞的分离培养与鉴定及性腺激素对其增殖的影响[J].江苏农业学报,2015,(04):834-839.[doi:10.3969/j.issn.1000-4440.2015.04.020]
　YE You-rong,ZHANG Lu,SUN Jie,et al.Culture and identification of endometrial cells of laying hen and effects of sex hormones on the proliferation[J].,2015,(04):834-839.[doi:10.3969/j.issn.1000-4440.2015.04.020]
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蛋鸡子宫内膜细胞的分离培养与鉴定及性腺激素对其增殖的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年04期
页码:
834-839
栏目:
畜牧兽医·水产养殖
出版日期:
2015-08-31

文章信息/Info

Title:
Culture and identification of endometrial cells of laying hen and effects of sex hormones on the proliferation
作者:
叶幼荣; 张璐; 孙杰; 桑永明; 赵宗胜
(石河子大学动物科技学院，新疆石河子832003)
Author(s):
YE You-rong; ZHANG Lu; SUN Jie; SANG Song-ming; ZHAO Zong-sheng
(College of Animal Science and Technology, Shihezi University, Shihezi 832003, China)
关键词:
蛋鸡; 子宫内膜; 上皮细胞; 基质细胞; 性激素; 体外增殖
Keywords:
laying hen; endometrium; epithelial cell; stromal cell; sex hormone; proliferation in vitro
分类号:
Q38
DOI:
10.3969/j.issn.1000-4440.2015.04.020
文献标志码:
A
摘要:
为探讨蛋鸡子宫内膜细胞的形态学特点、生长规律，本试验采用Ⅰ型胶原酶消化的方法获取蛋鸡子宫内膜上皮细胞和基质细胞，采用免疫组织化学法进行细胞鉴定，采用3-(4，5-二甲基噻唑-2)-2，5-二苯基四氮唑溴盐比色（MTT）法测得细胞生长曲线，并研究不同浓度水平的性腺激素对子宫内膜上皮细胞和基质细胞体外增殖的影响。结果显示，该方法培养的上皮细胞和基质细胞分别对角蛋白质ck18单克隆抗体和波形蛋白质抗体呈阳性；蛋鸡子宫内膜上皮细胞和基质细胞在接种后第2 d开始进入对数生长期，第7 d进入平台期；孕酮（100 nmol/L）能显著促进子宫内膜上皮细胞和基质细胞体外增殖；孕酮（100 nmol/L）和10 nmol/L、100 nmol/L雌激素组合对子宫内膜上皮细胞和基质细胞体外增殖均有显著促进作用。表明本研究成功培养了蛋鸡子宫内膜上皮细胞和基质细胞，并证实这2种细胞的细胞周期均为7 d，一定浓度的孕酮和雌激素及其组合对子宫内膜上皮细胞和基质细胞的体外增殖均有促进作用。
Abstract:
Endometrial epithelial and stromal cells were isolated and identified by type I collagenase digestion and immunohistochemistry, respectively. The growth curves were constructed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide （MTT） assay. The effects of different concentrations of sex hormones, estrogen (E2) and progesterone (P4), on the proliferation of the endometrial epithelial and stromal cells in vitro was studied. The epithelial and stromal cells were tested positive to keratin ck18 and vimentin antibody. The growth rates of chicken endometrial epithelial and stromal cells began to increase logarithmically on day 2, reaching the plateau phase on day 7. The endometrial cells and stromal cells treated with 100 nmol/L P4 proliferated at a significantly higher rate than the control. However, the endometrial epithelial cells and stromal cells treated with 100 nmol/L or 10 nmol/L E2 plus 100 nmol/L P4 proliferated at a significantly higher rate than the control.

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备注/Memo

备注/Memo:
收稿日期：2015-01-12 基金项目：国家自然科学基金项目（31060305）；教育部高校博士点基金项目（201065181200003） 作者简介：叶幼荣（1989-），女，河南信阳人，硕士，研究方向为动物遗传育种与繁殖。（Tel）13279932993;(E-mail)648510013@qq.com 通讯作者：孙杰，(E-mail)sunjie_shzu@126.com
更新日期/Last Update: 2015-08-31