[1]李大命,张彤晴,唐晟凯,等.太湖大银鱼(Protosalanx chinensis)细胞色素b基因序列多态性分析[J].江苏农业学报,2015,(04):840-845.[doi:10.3969/j.issn.1000-4440.2015.04.021]
　LI Da-ming,ZHANG Tong-qing,TANG Sheng-kai,et al.Genetic polymorphism of mitochondrial DNA cytochrome b (cytb) sequences of Protosalanx chinensis in Lake Taihu[J].,2015,(04):840-845.[doi:10.3969/j.issn.1000-4440.2015.04.021]
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太湖大银鱼(Protosalanx chinensis)细胞色素b基因序列多态性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2015年04期
页码:
840-845
栏目:
畜牧兽医·水产养殖
出版日期:
2015-08-31

文章信息/Info

Title:
Genetic polymorphism of mitochondrial DNA cytochrome b (cytb) sequences of Protosalanx chinensis in Lake Taihu
作者:
李大命; 张彤晴; 唐晟凯; 钟立强
（江苏省淡水水产研究所，江苏省内陆水域渔业资源重点实验室,江苏南京210017）
Author(s):
LI Da-ming; ZHANG Tong-qing; TANG Sheng-kai; ZHONG Li-qiang
(Freshwater Fisheries Research Institute of Jiangsu Province/Key Laboratory of Fisheries Resources in Inland Water, Nanjing 210017, China)
关键词:
大银鱼; 细胞色素b基因; 遗传多样性; 太湖
Keywords:
Protosalanx chinensis; cyt b; genetic diversity; Lake Taihu
分类号:
S964.1
DOI:
10.3969/j.issn.1000-4440.2015.04.021
文献标志码:
A
摘要:
为科学保护太湖大银鱼种质资源，制定合理的野生资源保护策略，实现太湖大银鱼资源的可持续发展和利用，以细胞色素b基因(cyt b)作为分子标记，对太湖大银鱼野生群体进行遗传多样性分析。通过PCR扩增与序列测定获得了cyt b基因全长序列(1 141 bp)。在35条同源cyt b基因序列中， A、T、G和C碱基的平均含量分别为21.67%、29.25%、16.71%和32.36%，A＋T的含量(50.92%)略高于G＋C的含量(49.07%)。Cyt b中共检出13个变异位点，总变异率为1.14%，其中9个为简约信息位点，4个为单一信息位点。35条cyt b序列共定义12个单倍型，单倍型之间的平均遗传距离为0.003，NJ法构建的单倍型系统进化树显示所有单倍型聚为一支，单倍型多样性指数和核苷酸多样性指数分别为 0.850±0.045和0.002 96±0.000 17，平均核苷酸差异数为3.378。中性检验分析结果显示Tajima’s D值为0.224 52，且差异不显著(P>0.10)，这表明太湖大银鱼种群稳定，没有经历种群扩张。
Abstract:
To protect Protosalanx chinensis germplasm, and maintain its sustainable develepment in Lake Taihu, the cytochrome b (cyt b) gene marker was selected for the genetic diverisity analysis. The completed mtDNA cyt b gene sequence (1 141 bp) of P.chinensis were obtained through PCR amplification and sequence analysis. The average contents of base A, T, G and C in 35 homological cyt b genes were 21.67%, 29.25%, 16.71% and 32.36%, respectively, with content of A＋T (50.92%) a little higher than that of G＋C (49.07%). Thirteen variation sites were detected, acconuting for 1.14% of the total variations, of which nine were parsimony informative sites and four were singleton informative sites. Twelve haplotypes were identified in 35 individuals of P.chinensis, with the average genetic distance among cyt b haplotypes being 0.003， the haplotype diversity and nucleodide diversity being 0.850±0.045 and 0.002 96±0.000 17, respectively, and the average number of nucleotide differences being 3.378. The phylogenetic tree revealed that twelve cyt b haplotypes were clustered into one clade. The positive Tajima′s D value (0.224 52) of neutrality test suggests that P.chinensis has a stable population without experiencing population expansion.

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备注/Memo

备注/Memo:
收稿日期：2014-11-22 基金项目：江苏省青年科学基金项目(BK2012488)；江苏省内陆省管渔业水域资源与环境监测(江苏省海洋与渔业局, 2013)项目 作者简介：李大命(1981-)，男，河南汝南县人，博士，助理研究员，研究方向为湖泊渔业资源与环境。（E-mail）ldm8212@126.com 通讯作者：张彤晴，（E-mail）zhtq3@126.com
更新日期/Last Update: 2015-08-31